Use of adaptive optics to increase nonlinear imaging signal in mouse bone morrow

2008 ◽  
Author(s):  
Yaopeng Zhou ◽  
Thomas Bifano ◽  
Charles Lin
Author(s):  
Karen F. Han

The primary focus in our laboratory is the study of higher order chromatin structure using three dimensional electron microscope tomography. Three dimensional tomography involves the deconstruction of an object by combining multiple projection views of the object at different tilt angles, image intensities are not always accurate representations of the projected object mass density, due to the effects of electron-specimen interactions and microscope lens aberrations. Therefore, an understanding of the mechanism of image formation is important for interpreting the images. The image formation for thick biological specimens has been analyzed by using both energy filtering and Ewald sphere constructions. Surprisingly, there is a significant amount of coherent transfer for our thick specimens. The relative amount of coherent transfer is correlated with the relative proportion of elastically scattered electrons using electron energy loss spectoscopy and imaging techniques.Electron-specimen interactions include single and multiple, elastic and inelastic scattering. Multiple and inelastic scattering events give rise to nonlinear imaging effects which complicates the interpretation of collected images.


2010 ◽  
Vol 34 (8) ◽  
pp. S43-S43
Author(s):  
Wei‑ying Zou ◽  
Bei Yang ◽  
Xiuli Ni ◽  
Da‑lei Zhang ◽  
Lei Wu ◽  
...  

Author(s):  
Carlos Correia ◽  
Henri-Francois Raynaud ◽  
Caroline Kulcsar ◽  
Jean-Marc Conan
Keyword(s):  

2020 ◽  
Vol 10 (10) ◽  
pp. 52-58
Author(s):  
Sergey M. AFONIN ◽  

An electroelastic actuator for nanomechatronics is used in nanotechnology, adaptive optics, microsurgery, microelectronics, and biomedicine to actuate or control mechanisms, systems based on the electroelastic effect, and to convert electrical signals into mechanical displacements and forces. In nanomechatronic systems, a piezoactuator is used in scanning microscopy, laser systems, in astronomy for precision alignment, for compensation of temperature, gravitational deformations and atmospheric turbulence, focusing, and stabilizing the image. In this study, a condition for absolute stability of an electroelastic actuator control system for nanomechatronics under deterministic and random inputs is obtained. A number of equilibrium positions in an electroelastic actuator mechatronic control system are found, the totality of which is represented by a straight line segment. The electroelastic actuator’s deformation control system dead band relative width is determined for the actuator’s symmetric and asymmetric hysteresis characteristics. Under deterministic inputs and with fulfilling the condition for the derivative of the nonlinear hysteresis actuator deformation characteristic, the set of equilibrium positions of the electroelastic actuator control system for nanomechatronics is absolutely stable. Under random inputs, the system absolute stability with respect to the mathematical expectations of the electroelastic actuator mechatronic control system equilibrium positions has been determined subject to fulfilling the condition on the derivative of the actuator hysteresis characteristic.


1989 ◽  
Author(s):  
R. A. Craig ◽  
C. R. Batishko ◽  
J. L. Brimhall ◽  
W. T. Pawlewicz ◽  
K. A. Stahl ◽  
...  

1991 ◽  
Author(s):  
W. J. Gignac ◽  
R. R. Stephens ◽  
A. A. Narayanan ◽  
R. R. Craig ◽  
H. W. Yen
Keyword(s):  

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