scholarly journals Glucocorticoid counteracts cellular mechanoresponses by LINC01569-dependent glucocorticoid receptor–mediated mRNA decay

2021 ◽  
Vol 7 (9) ◽  
pp. eabd9923
Author(s):  
Huayu Zhu ◽  
Jun Li ◽  
Yize Li ◽  
Zhao Zheng ◽  
Hao Guan ◽  
...  
Keyword(s):  
Glucocorticoid Receptor ◽  
Messenger Rna ◽  
Noncoding Rna ◽  
Growth Response ◽  
Mrna Decay ◽  
Mechanical Stimuli ◽  
Early Growth Response ◽  
Cellular Mechanotransduction ◽  
Potential Strategy ◽  
Bone Morphogenic Protein 7

Mechanical stimuli on cells and mechanotransduction are essential in many biological and pathological processes. Glucocorticoid is an important hormone, roles, and mechanisms of which in cellular mechanotransduction remain unknown. Here, we report that glucocorticoid counteracted cellular mechanoresponses dependently on a novel long noncoding RNA (lncRNA), LINC01569. Further, LINC01569 mediated glucocorticoid effects on mechanotransduction by destabilizing messenger RNA (mRNA) of mechanosensors including early growth response protein 1 (EGR1), Cbp/P300-interacting transactivator 2 (CITED2), and bone morphogenic protein 7 (BMP7) in glucocorticoid receptor–mediated mRNA decay (GMD) manner. Mechanistically, LINC01569 directly bound to the GMD factor Y-box–binding protein 1 (YBX1). Then, the LINC01569-YBX1 complex was guided to the mRNAs of EGR1, CITED2, and BMP7 through specific LINC01569-mRNA interaction, thereby contributing to the successful assembly of GMD complex and triggering GMD. Our results uncovered roles of glucocorticoid in cellular mechanotransduction and novel lncRNA-dependent GMD machinery and provided potential strategy for early intervention in mechanical disorder–associated diseases.

Early Growth Response Protein 1 Mediates Long Noncoding RNA Arid2-IR to Promote Extracellular Matrix Production in Diabetic Kidney Disease

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 504-P ◽  
Author(s):  
YAN-LIN YANG ◽  
FANG HU ◽  
MENG XUE ◽  
YIJIE JIA ◽  
MEINA ZOU ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Kidney Disease ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Growth Response ◽  
Diabetic Kidney Disease ◽  
Early Growth ◽  
Early Growth Response ◽  
Extracellular Matrix Production ◽  
Matrix Production

scholarly journals Early growth response protein-1 upregulates long noncoding RNA Arid2-IR to promote extracellular matrix production in diabetic kidney disease

2019 ◽  
Vol 316 (3) ◽  
pp. C340-C352 ◽  
Author(s):  
Yan-Lin Yang ◽  
Fang Hu ◽  
Meng Xue ◽  
Yi-Jie Jia ◽  
Zong-Ji Zheng ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Kidney Disease ◽  
High Glucose ◽  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Growth Response ◽  
Diabetic Kidney Disease ◽  
Mesangial Cells ◽  
Early Growth ◽  
Early Growth Response

Diabetic kidney disease (DKD) has surpassed chronic glomerulonephritis as the leading cause of end-stage renal disease. Previously, we showed that early growth response protein-1 (Egr1) plays a key role in DKD by enhancing mesangial cell proliferation and extracellular matrix (ECM) production. The long noncoding RNA (lncRNA) AT-rich interactive domain 2-IR (Arid2-IR) has been identified as a mothers against decapentaplegic homolog 3 (Smad3)-associated lncRNA in unilateral ureteral obstructive kidney disease. However, the effect of Egr1 on Arid2-IR in the development of DKD is still unknown. In this study, we found that Arid2-IR was increased in mice with high-fat diet and streptozotocin-induced type 2 diabetes and in mouse mesangial cells cultured with high glucose to mimic diabetes. Knockdown of Arid2-IR in mouse mesangial cells reduced the high expression levels of collagen-α1(I) (Col1a1) and α-smooth muscle actin (α-SMA) induced by high glucose. Furthermore, Arid2-IR expression changed the increased expression of Col1a1 and α-SMA caused by overexpression of Egr1. Overall, these data suggest that increased Arid2-IR likely contributes to ECM production in DKD and that Egr1 promotes ECM production in DKD partly by upregulating Arid2-IR. Thus, Arid2-IR may be a new target in the treatment of DKD.

Role of Early Growth Response-1 in the Development of Alcohol-Induced Steatosis

2017 ◽  
Vol 10 (3) ◽  
pp. 179-185 ◽  
Author(s):  
Paul G. Thomes ◽  
Terrence Donohue
Keyword(s):  
Growth Response ◽  
Early Growth ◽  
Early Growth Response ◽  
Early Growth Response 1

Bone Marrow Mesenchymal Stem Cell mediated Ultrasmall Gold Nanoclusters and hNIS Gene Synergize Radiotherapy in Breast Cancer

2021 ◽  
Author(s):  
Lu Zhang ◽  
Benchao Zheng ◽  
Rui Guo ◽  
Ying Miao ◽  
Biao Li
Keyword(s):  
Breast Cancer ◽  
Bone Marrow ◽  
Stem Cell ◽  
Growth Response ◽  
Sodium Iodide ◽  
Gold Nanoclusters ◽  
Early Growth ◽  
Sodium Iodide Symporter ◽  
Early Growth Response ◽  
131I Treatment

The human sodium iodide symporter (hNIS) can be linked to the downstream of radiation-sensitive early growth response protein1 (Egr1) promoter, and activated by the Egr1 following 131I treatment. However, the...

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