Bone Marrow Mesenchymal Stem Cell mediated Ultrasmall Gold Nanoclusters and hNIS Gene Synergize Radiotherapy in Breast Cancer

2021 ◽  
Author(s):  
Lu Zhang ◽  
Benchao Zheng ◽  
Rui Guo ◽  
Ying Miao ◽  
Biao Li
Keyword(s):  
Breast Cancer ◽  
Bone Marrow ◽  
Stem Cell ◽  
Growth Response ◽  
Sodium Iodide ◽  
Gold Nanoclusters ◽  
Early Growth ◽  
Sodium Iodide Symporter ◽  
Early Growth Response ◽  
131I Treatment

The human sodium iodide symporter (hNIS) can be linked to the downstream of radiation-sensitive early growth response protein1 (Egr1) promoter, and activated by the Egr1 following 131I treatment. However, the...

scholarly journals Mesenchymal Stem Cell-Mediated Delivery of the Sodium Iodide Symporter Supports Radionuclide Imaging and Treatment of Breast Cancer

Stem Cells ◽  
2011 ◽  
Vol 29 (7) ◽  
pp. 1149-1157 ◽  
Author(s):  
Roisin M. Dwyer ◽  
James Ryan ◽  
Ronan J. Havelin ◽  
John C. Morris ◽  
Brian W. Miller ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Radionuclide Imaging ◽  
Sodium Iodide ◽  
Sodium Iodide Symporter

The Role of Early Growth Response 1 in the Prognostic Value and Cell Migration of Breast Cancer

2021 ◽  
Author(s):  
Leiyu Hao ◽  
Fengru Huang ◽  
Xinqian Yu ◽  
Bujie Xu ◽  
Yan Liu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Protein Expression ◽  
Prognostic Value ◽  
Growth Response ◽  
Expression Patterns ◽  
Early Growth ◽  
The Cancer Genome Atlas ◽  
Early Growth Response

Abstract Background: Early growth response family members (EGRs), EGR1-4, have increasingly attracted attention in multiple cancers. However, the exact expression patterns and prognostic values of EGRs in the progress of breast cancer (BRCA) remain largely unknown. Methods: The mRNA expression and prognostic characteristics of EGRs were examined by the Cancer Genome Atlas (TCGA), Oncomine and Kaplan-Meier plotter. Enrichment analyses were conducted based on protein-protein interaction (PPI) network. The Tumor Immune Estimation Resource (TIMER) database and MethSurv were further explored. The protein expression level of EGR1 and cell migration were measured by Western blotting, immunohistochemistry, wound-healing assay and Boyden chamber assay in BRCA. Results: The transcriptional levels of EGR1/2/3 displayed significantly low expression in BRCA compared to that in normal tissues, while EGR4 was shown adverse expression pattern. Survival analysis revealed up-regulated EGR1-4 were remarkably associated with favorable relapse-free survival (RFS). A close correlation with specific tumor-infiltrating immune cells (TIICs) and several CpG sites of EGRs were exhibited. Immunohistochemistry assays showed that the protein expression of EGR1 was remarkably downregulated in BRCA compared to that in paracancerous tissues. Cell migration of MCF10A cells was increased after the silence of EGR1 by siRNA transfection.Conclusions: This study provides a novel insight to the role of EGR1 in the prognostic value and cell migration of BRCA.

scholarly journals Early growth response 1 regulates hematopoietic support and proliferation in human primary bone marrow stromal cells

Haematologica ◽  
2019 ◽  
Vol 105 (5) ◽  
pp. 1206-1215 ◽  
Author(s):  
Hongzhe Li ◽  
Hooi-Ching Lim ◽  
Dimitra Zacharaki ◽  
Xiaojie Xian ◽  
Keane J.G. Kenswil ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Growth Response ◽  
Early Growth ◽  
Marrow Stromal Cells ◽  
Early Growth Response ◽  
Primary Bone ◽  
Early Growth Response 1 ◽  
Primary Bone Marrow

scholarly journals A Risk Prediction Model for Breast Cancer Based on Immune Genes Related to Early Growth Response Proteins Family

2021 ◽  
Vol 7 ◽  
Author(s):  
Xin Zhou ◽  
Fang-yuan Zhang ◽  
Yan Liu ◽  
Dong-xin Wei
Keyword(s):  
Breast Cancer ◽  
Prediction Model ◽  
Risk Prediction ◽  
Growth Response ◽  
Methylation Status ◽  
Genetic Alterations ◽  
Early Growth ◽  
Risk Prediction Model ◽  
Early Growth Response ◽  
Immune Signature

Early growth response proteins (EGRs), a transcriptional regulatory family comprised of EGR1, EGR2, EGR3, and EGR 4, are reportedly involved in a vast array of functions. However, EGRs, as a whole, are rarely studied in breast cancer cases. This research was performed based on public datasets. The results demonstrated that, except EGR4, the other EGRs were differentially expressed genes in breast cancer. Subsequently, this study determined the prognosis significance of the EGR family, higher expression levels of EGRs indicating better overall survival (OS) and disease-free survival (DFS), except EGR4. So we attempted to explore the potential mechanism behind the prognostic value of EGRs. At the DNA level, however, neither DNA methylation status nor genetic alterations of EGRs contributed to the prognosis significance. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that EGRs were involved in several immune-related functions. Afterward, we assessed the correlation between EGRs and the immune system before establishing a risk prediction model with a 14-gene immune signature associated with EGRs, a prognostic nomogram predicting individuals’ 1-, 3-, and 5-year survival probabilities. The risk score was an independent prognosis predictor in the breast cancer cohorts. This study evidenced EGRs’ significance for tumor immunity, demonstrating that the EGR family may be a potential immunotherapeutic target for breast cancer. The 14-gene immune signature is a promising prognostic biomarker in breast cancer.

scholarly journals The Transcription Factor Early Growth Response 1 (Egr-1) Advances Differentiation of Pre-B and Immature B Cells

1998 ◽  
Vol 188 (12) ◽  
pp. 2215-2224 ◽  
Author(s):  
Adelheid Dinkel ◽  
Klaus Warnatz ◽  
Birgit Ledermann ◽  
Antonius Rolink ◽  
Peter F. Zipfel ◽  
...  
Keyword(s):  
Bone Marrow ◽  
B Cells ◽  
B Cell ◽  
B Lymphocytes ◽  
Growth Response ◽  
Early Growth ◽  
Early Growth Response ◽  
Immature B Cells ◽  
Early Growth Response 1 ◽  
I Cells

In mature B lymphocytes, the zinc finger transcription factor early growth response 1 (Egr-1) is one of the many immediate-early genes induced upon B cell antigen receptor engagement. However, its role during earlier stages of lymphopoiesis has remained unclear. By examining bone marrow B cell subsets, we found Egr-1 transcripts in pro/pre-B and immature B lymphocytes, and Egr-1 protein in pro/pre-B–I cells cultivated on stroma cells in the presence of interleukin (IL)-7. In recombinase-activating gene (RAG)-2–deficient mice overexpressing an Egr-1 transgene in the B lymphocyte lineage, pro/pre-B–I cells could differentiate past a developmental block at the B220low BP-1− stage to the stage of B220low BP-1+ pre-B–I cells, but not further to the B220low BP-1+ CD25+ stage of pre-B–II cells. Therefore, during early B lymphopoiesis progression from the B220low BP-1− IL-2R− pro/pre-B–I stage to the B220low BP-1+ IL-2R+ pre-B–II stage seems to occur in at least two distinct steps, and the first step to the stage of B220low BP-1+ pre-B–I cells can be promoted by the overexpression of Egr-1 alone. Wild-type mice expressing an Egr-1 transgene had increased proportions of mature immunoglobulin (Ig)M+ B220high and decreased proportions of immature IgM+ B220low bone marrow B cells. Since transgenic and control precursor B cells show comparable proliferation patterns, overexpression of Egr-1 seems also to promote entry into the mature B cell stage. Analysis of changes in the expression pattern of potential Egr-1 target genes revealed that Egr-1 enhances the expression of the aminopeptidase BP-1/6C3 in pre-B and immature B cells and upregulates expression of the orphan nuclear receptor nur77 in IgM+ B cells.

scholarly journals Radiation Induced Apoptosis of Murine Bone Marrow Cells Is Independent of Early Growth Response 1 (EGR1)

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0169767 ◽  
Author(s):  
Karine Z. Oben ◽  
Beth W. Gachuki ◽  
Sara S. Alhakeem ◽  
Mary K. McKenna ◽  
Ying Liang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Response ◽  
Bone Marrow Cells ◽  
Early Growth ◽  
Murine Bone Marrow ◽  
Early Growth Response ◽  
Radiation Induced ◽  
Induced Apoptosis ◽  
Early Growth Response 1 ◽  
Marrow Cells

scholarly journals The Early Growth Response Factor-1 Contributes to Interleukin-13 Production by Mast Cells in Response to Stem Cell Factor Stimulation

2008 ◽  
Vol 5 (2) ◽  
pp. 163-171 ◽  
Author(s):  
Bo Li ◽  
Jason Berman ◽  
Ping Wu ◽  
Fang Liu ◽  
Jin-Tian Tang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mast Cells ◽  
Stem Cell Factor ◽  
Growth Response ◽  
Early Growth ◽  
Response Factor ◽  
Early Growth Response ◽  
Interleukin 13
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