A Medium for the Study of the Bacterial Oxidation of Ferrous Iron

Ferrous iron oxidation studies in the presence of activated carbon were conducted at 30 °C in basal medium at pH 1.6 with a pure strain of Acidithiobacillus ferrooxidans. Two-chamber modified shake flasks were used in these experiments, which prevented direct contact between the microorganisms and the carbon contained in the flasks. This design permitted an accurate determination of bacterial population during the experiment and enabled the involvement of ferric iron reduction with carbon to be evidenced. Notably, iron was initially added as ferric iron in a concentration of 3 g/L. It could be observed that bacteria could grow in this condition evidencing that bacteria was in fact oxidizing ferrous ion produced from reduction of ferric by carbon. From complementary experiments in which activated carbon was contacted with abiotic solutions containing ferric ion in the concentration range 0.1 – 1.2 g/l, the chemical reductive action of carbon of ferric iron was confirmed and a kinetic expression for this reaction was determined. A mathematical model was developed which incorporated expressions for the kinetic of bacterial oxidation of ferrous ion and the chemical reduction of ferric ion. This model enabled the prediction of the rate of bacterial growth and ferrous ion oxidation in a bioreactor as a function of the initial concentrations of iron, activated carbon and bacterial population. Results in this work imply that the observed variations in activity observed by other authors during bacterial oxidation of ferrous iron with A. ferrooxidans adsorbed on carbon can be in fact related to bacterial utilization of supplementary ferrous iron produced by the chemical action of carbon, phenomenon which is not explicitly accounted for.

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Bacterial oxidation of ferrous iron at low temperatures

Biotechnology and Bioengineering ◽

10.1002/bit.21371 ◽

2007 ◽

Vol 97 (6) ◽

pp. 1470-1478 ◽

Cited By ~ 44

Author(s):

Daniel Kupka ◽

Olena I. Rzhepishevska ◽

Mark Dopson ◽

E. Börje Lindström ◽

Olia V. Karnachuk ◽

...

Keyword(s):

Low Temperatures ◽

Ferrous Iron ◽

Bacterial Oxidation

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Bacterial oxidation of ferrous iron by Acidithiobacillus ferrooxidans in the pH range 2.5–7.0

Hydrometallurgy ◽

10.1016/s0304-386x(03)00151-8 ◽

2003 ◽

Vol 71 (1-2) ◽

pp. 149-158 ◽

Cited By ~ 57

Author(s):

Gabriel Meruane ◽

Tomás Vargas

Keyword(s):

Acidithiobacillus Ferrooxidans ◽

Ferrous Iron ◽

Bacterial Oxidation ◽

Ph Range

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PENDEKATAN EKSPLORASI RESERVOIR ONSHORE MELALUI PEMETAAN REMBESAN MIKRO HIDROKARBON DENGAN CITRA HIPERSPEKTRAL

Seminar Nasional Geomatika ◽

10.24895/sng.2018.3-0.1042 ◽

2019 ◽

Vol 3 ◽

pp. 1181

Author(s):

Muhamad Iqbal Januadi Putra

Keyword(s):

Ferrous Iron

Kapabilitas citra satelit hiperspektral yang memiliki keunggulan spektral sangat berpotensi untuk dimanfaatkan dalam eksplorasi keberadaan reservoir onshore. Kemampuan ini dilakukan melalui perekaman spektral fenomena rembesan mikro hidrokarbon yang menimbulkan ekspresi anomali spektral tanah dan menjadi kunci keberadaan sumber hidrokarbon. Dalam penelitian ini, Sub-Cekungan Jatibarang dipilih sebagai wilayah penelitian. Berdasarkan teori spektral rembesan mikro hidrokarbon, citra Hyperion dapat digunakan sebagai alat untuk mendeteksi keberadaan fenomena rembesan mikro hidrokarbon sebagai penduga keberadaan reservoir onshore karena memiliki spektrum panjang gelombang yang peka terhadap objek anomali hidrokarbon. Sehingga, penelitian ini bertujuan untuk mendeteksi area rembesan mikro hidrokarbon dengan citra Hyperion di Cekungan Jatibarang. Hydrocarbon Index (HI) yang memanfaatkan saluran 155 (1.699 nm), 158 (1.729 nm), dan 160 (1.749 nm) digunakan sebagai algoritma untuk medeteksi area rembesan mikro hidrokarbon di Sub-Cekungan Jatibarang. Gejala anomali alterasi kaolinite, ferrous iron dan gejala anomali vegetasi dengan indeks NDVI digunakan sebagai pembanding akurasi HI. Hasil penelitian ini menunjukkan adanya sebaran rembesan mikro hidrokarbon di wilayah Kecamatan Tukdana (Indramayu), Kecamatan Pasekan (Indramayu), dan Kecamatan Ligung (Majalengka). Area sebaran rembesan mikro hidrokarbon teraglomerasi pada wilayah pesisir utara Kecamatan Pasekan. Secara umum, keberadaan rembesan mikro hidrokarbon berkorelasi dengan tingkat anomali alterasi kaolinite, ferrous iron, dan anomali vegetasi.

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Synthesis and Breakdown of the Universal Precursors to Biological Metabolism Promoted by Ferrous Iron

10.26434/chemrxiv.7178930.v1 ◽

2018 ◽

Cited By ~ 1

Author(s):

Kamila B. Muchowska ◽

Sreejith Jayasree VARMA ◽

Joseph Moran

Keyword(s):

Amino Acids ◽

Chemical Reaction ◽

Metabolic Pathways ◽

Ferrous Iron ◽

Reaction Network ◽

Tca Cycle ◽

Chemical Reaction Network ◽

Metabolic Precursors ◽

Tca Cycle Intermediates

How core biological metabolism initiated and why it uses the intermediates, reactions and pathways that it does remains unclear. Life builds its molecules from CO2 and breaks them down to CO2 again through the intermediacy of just five metabolites that act as the hubs of biochemistry. Here, we describe a purely chemical reaction network promoted by Fe2+ in which aqueous pyruvate and glyoxylate, two products of abiotic CO2 reduction, build up nine of the eleven TCA cycle intermediates, including all five universal metabolic precursors. The intermediates simultaneously break down to CO2 in a life-like regime resembling biological anabolism and catabolism. Introduction of hydroxylamine and Fe0 produces four biological amino acids. The network significantly overlaps the TCA/rTCA and glyoxylate cycles and may represent a prebiotic precursor to these core metabolic pathways.

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