Stimulation of Amino Acid Transport in Isolated Diaphragm by Growth Hormone Added in vitro

Science ◽  
1959 ◽  
Vol 130 (3389) ◽  
pp. 1653-1654 ◽  
Author(s):  
J. L. KOSTYO ◽  
J. HOTCHKISS ◽  
E. KNOBIL
Keyword(s):  
Growth Hormone ◽  
Amino Acid ◽  
Amino Acid Transport ◽  
Acid Transport ◽  
Stimulation Of

Interaction between growth hormone and rat muscle in vitro

1961 ◽  
Vol 200 (4) ◽  
pp. 675-678 ◽  
Author(s):  
J. L. Kostyo ◽  
J. E. Schmidt
Keyword(s):  
Growth Hormone ◽  
Amino Acid ◽  
Amino Acid Transport ◽  
Growth Hormones ◽  
Hormone Concentration ◽  
Acid Transport ◽  
Rat Muscle ◽  
Resultant Effect ◽  
Initial Interaction

Hypophysectomized rat diaphragms, which were immersed briefly in dilute solutions of growth hormone and then washed thoroughly, subsequently transported α-aminoisobutyric acid-1-C14 at a greater rate than the controls. Growth hormones of bovine, porcine, simian and human origins were all effective. Increasing either the hormone concentration or the length of time that the diaphragms were immersed in growth hormone solutions increased the effect on amino acid transport. Prolonged washing of the diaphragms following exposure to growth hormone did not reduce the magnitude of the effect on amino acid transport. Moreover, reducing the temperature of the growth hormone solutions did not diminish the resultant effect on amino acid transport. From these results, it was concluded that the initial interaction between growth hormone and rat muscle in vitro occurs rapidly and the modification produced by this interaction is relatively stable.

Stimulation of transport of alpha-aminoisobutyric acid into the testes of immature mice in vivo by follicle-stimulating hormone

1982 ◽  
Vol 100 (1) ◽  
pp. 137-142
Author(s):  
Nila Oza ◽  
Sarah J. Meanock ◽  
A. G. Davies
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Amino Acid Transport ◽  
Specific Activity ◽  
Follicle Stimulating Hormone ◽  
Acid Transport ◽  
Aminoisobutyric Acid ◽  
Old Mice ◽  
Stimulation Of

Abstract. Groups of immature mice were injected sc with radiocarbon-labelled alpha-aminoisobutyric acid (AIB) after being given a single sc injection of hFSH or of 0.9% saline. As an index of the transport of AIB, the specific activity of isotope was measured in homogenates of testis and of liver. FSH treatment caused statistically significant increases in the specific activity of isotope in the testes and in the ratio of testicular to liver specific activity. The effect was greatest in 9-day-old mice injected with FSH 16 h before removal of the testes. Uptake of labelled AIB was not stimulated after administration of hCG or testosterone. Doses of cycloheximide sufficient to reduce the rate of protein synthesis by over 99% did not impair testicular uptake of labelled AIB or the influence of FSH on AIB uptake. These results suggest that FSH stimulates amino acid transport into cells of the immature testis and that this action is independent of the stimulatory effect of FSH on testicular protein synthesis.

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