Interleukin-2 receptor beta chain gene: generation of three receptor forms by cloned human alpha and beta chain cDNA's

Science ◽  
1989 ◽  
Vol 244 (4904) ◽  
pp. 551-556 ◽  
Author(s):  
M Hatakeyama ◽  
M Tsudo ◽  
S Minamoto ◽  
T Kono ◽  
T Doi ◽  
...  
Keyword(s):  
Interleukin 2 ◽  
Chain Gene ◽  
Beta Chain ◽  
Interleukin 2 Receptor ◽  
Receptor Beta

scholarly journals Human interleukin 2 receptor beta-chain gene: chromosomal localization and identification of 5' regulatory sequences.

1990 ◽  
Vol 87 (9) ◽  
pp. 3440-3444 ◽  
Author(s):  
J. R. Gnarra ◽  
H. Otani ◽  
M. G. Wang ◽  
O. W. McBride ◽  
M. Sharon ◽  
...  
Keyword(s):  
Chromosomal Localization ◽  
Interleukin 2 ◽  
Chain Gene ◽  
Regulatory Sequences ◽  
Beta Chain ◽  
Interleukin 2 Receptor ◽  
Receptor Beta

scholarly journals Characterization of the human interleukin-2 receptor beta-chain gene promoter: regulation of promoter activity by ets gene products.

1993 ◽  
Vol 13 (10) ◽  
pp. 6201-6210 ◽  
Author(s):  
J X Lin ◽  
N K Bhat ◽  
S John ◽  
W S Queale ◽  
W J Leonard
Keyword(s):  
Binding Site ◽  
Phorbol Myristate Acetate ◽  
Promoter Activity ◽  
Interleukin 2 ◽  
Chain Gene ◽  
Beta Chain ◽  
Myristate Acetate ◽  
Interleukin 2 Receptor ◽  
Mg63 Cells ◽  
Beta Gene

The interleukin-2 receptor (IL-2R) beta chain (IL-2R beta) is an essential signaling component of high- and intermediate-affinity IL-2Rs. Our laboratory previously reported that a DNA fragment containing 857 bp of 5'-flanking sequence of the human IL-2R beta gene exhibited promoter activity. We have now further characterized the promoter and delineated cis-acting regulatory regions. The region downstream of -363 is critical for basal and phorbol myristate acetate-inducible IL-2R beta promoter activity and contains at least three enhancer-like regions. Among them, the -56 to -34 enhancer was the most potent and had high-level activity in two T-cell lines but not in nonlymphoid HeLaS3 and MG63 cells. This enhancer contains a GGAA Ets binding site which bound two Ets family proteins, Ets-1 and GA-binding protein in vitro. Mutation of the Ets motif strongly diminished both promoter and enhancer activities. We conclude that this Ets binding site plays a key role in regulating basal and phorbol myristate acetate-inducible IL-2R beta promoter activity and may also contribute to tissue-specific expression of the IL-2R beta gene.

Abstract 2326: LTX-315, an oncolytic peptide, increases anticancer immunity mediated by CTLA4 blockade in an interleukin-2 receptor beta-chain-dependent manner

2016 ◽  
Author(s):  
Takahiro Yamazaki ◽  
Marie Vetizou ◽  
Camila Flores ◽  
Aurelien Marabelle ◽  
Baldur Sveinbjørnsson ◽  
...  
Keyword(s):  
Interleukin 2 ◽  
Dependent Manner ◽  
Beta Chain ◽  
Interleukin 2 Receptor ◽  
Ctla4 Blockade ◽  
Receptor Beta ◽  
Anticancer Immunity

scholarly journals c-fos gene induction by interleukin 2: identification of the critical cytoplasmic regions within the interleukin 2 receptor beta chain.

1992 ◽  
Vol 89 (6) ◽  
pp. 2022-2026 ◽  
Author(s):  
M. Hatakeyama ◽  
A. Kawahara ◽  
H. Mori ◽  
H. Shibuya ◽  
T. Taniguchi
Keyword(s):  
Interleukin 2 ◽  
Gene Induction ◽  
Beta Chain ◽  
Interleukin 2 Receptor ◽  
Receptor Beta

Characterization of the human interleukin-2 receptor beta-chain gene promoter: regulation of promoter activity by ets gene products

1993 ◽  
Vol 13 (10) ◽  
pp. 6201-6210
Author(s):  
J X Lin ◽  
N K Bhat ◽  
S John ◽  
W S Queale ◽  
W J Leonard
Keyword(s):  
Binding Site ◽  
Phorbol Myristate Acetate ◽  
Promoter Activity ◽  
Interleukin 2 ◽  
Chain Gene ◽  
Beta Chain ◽  
Myristate Acetate ◽  
Interleukin 2 Receptor ◽  
Mg63 Cells ◽  
Beta Gene

The interleukin-2 receptor (IL-2R) beta chain (IL-2R beta) is an essential signaling component of high- and intermediate-affinity IL-2Rs. Our laboratory previously reported that a DNA fragment containing 857 bp of 5'-flanking sequence of the human IL-2R beta gene exhibited promoter activity. We have now further characterized the promoter and delineated cis-acting regulatory regions. The region downstream of -363 is critical for basal and phorbol myristate acetate-inducible IL-2R beta promoter activity and contains at least three enhancer-like regions. Among them, the -56 to -34 enhancer was the most potent and had high-level activity in two T-cell lines but not in nonlymphoid HeLaS3 and MG63 cells. This enhancer contains a GGAA Ets binding site which bound two Ets family proteins, Ets-1 and GA-binding protein in vitro. Mutation of the Ets motif strongly diminished both promoter and enhancer activities. We conclude that this Ets binding site plays a key role in regulating basal and phorbol myristate acetate-inducible IL-2R beta promoter activity and may also contribute to tissue-specific expression of the IL-2R beta gene.

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