scholarly journals Characterization of the human interleukin-2 receptor beta-chain gene promoter: regulation of promoter activity by ets gene products.

1993 ◽  
Vol 13 (10) ◽  
pp. 6201-6210 ◽  
Author(s):  
J X Lin ◽  
N K Bhat ◽  
S John ◽  
W S Queale ◽  
W J Leonard
Keyword(s):  
Binding Site ◽  
Phorbol Myristate Acetate ◽  
Promoter Activity ◽  
Interleukin 2 ◽  
Chain Gene ◽  
Beta Chain ◽  
Myristate Acetate ◽  
Interleukin 2 Receptor ◽  
Mg63 Cells ◽  
Beta Gene

The interleukin-2 receptor (IL-2R) beta chain (IL-2R beta) is an essential signaling component of high- and intermediate-affinity IL-2Rs. Our laboratory previously reported that a DNA fragment containing 857 bp of 5'-flanking sequence of the human IL-2R beta gene exhibited promoter activity. We have now further characterized the promoter and delineated cis-acting regulatory regions. The region downstream of -363 is critical for basal and phorbol myristate acetate-inducible IL-2R beta promoter activity and contains at least three enhancer-like regions. Among them, the -56 to -34 enhancer was the most potent and had high-level activity in two T-cell lines but not in nonlymphoid HeLaS3 and MG63 cells. This enhancer contains a GGAA Ets binding site which bound two Ets family proteins, Ets-1 and GA-binding protein in vitro. Mutation of the Ets motif strongly diminished both promoter and enhancer activities. We conclude that this Ets binding site plays a key role in regulating basal and phorbol myristate acetate-inducible IL-2R beta promoter activity and may also contribute to tissue-specific expression of the IL-2R beta gene.

Characterization of the human interleukin-2 receptor beta-chain gene promoter: regulation of promoter activity by ets gene products

1993 ◽  
Vol 13 (10) ◽  
pp. 6201-6210
Author(s):  
J X Lin ◽  
N K Bhat ◽  
S John ◽  
W S Queale ◽  
W J Leonard
Keyword(s):  
Binding Site ◽  
Phorbol Myristate Acetate ◽  
Promoter Activity ◽  
Interleukin 2 ◽  
Chain Gene ◽  
Beta Chain ◽  
Myristate Acetate ◽  
Interleukin 2 Receptor ◽  
Mg63 Cells ◽  
Beta Gene

The interleukin-2 receptor (IL-2R) beta chain (IL-2R beta) is an essential signaling component of high- and intermediate-affinity IL-2Rs. Our laboratory previously reported that a DNA fragment containing 857 bp of 5'-flanking sequence of the human IL-2R beta gene exhibited promoter activity. We have now further characterized the promoter and delineated cis-acting regulatory regions. The region downstream of -363 is critical for basal and phorbol myristate acetate-inducible IL-2R beta promoter activity and contains at least three enhancer-like regions. Among them, the -56 to -34 enhancer was the most potent and had high-level activity in two T-cell lines but not in nonlymphoid HeLaS3 and MG63 cells. This enhancer contains a GGAA Ets binding site which bound two Ets family proteins, Ets-1 and GA-binding protein in vitro. Mutation of the Ets motif strongly diminished both promoter and enhancer activities. We conclude that this Ets binding site plays a key role in regulating basal and phorbol myristate acetate-inducible IL-2R beta promoter activity and may also contribute to tissue-specific expression of the IL-2R beta gene.

Interleukin-2 receptor beta chain gene: generation of three receptor forms by cloned human alpha and beta chain cDNA's

Science ◽  
1989 ◽  
Vol 244 (4904) ◽  
pp. 551-556 ◽  
Author(s):  
M Hatakeyama ◽  
M Tsudo ◽  
S Minamoto ◽  
T Kono ◽  
T Doi ◽  
...  
Keyword(s):  
Interleukin 2 ◽  
Chain Gene ◽  
Beta Chain ◽  
Interleukin 2 Receptor ◽  
Receptor Beta

scholarly journals Human interleukin 2 receptor beta-chain gene: chromosomal localization and identification of 5' regulatory sequences.

1990 ◽  
Vol 87 (9) ◽  
pp. 3440-3444 ◽  
Author(s):  
J. R. Gnarra ◽  
H. Otani ◽  
M. G. Wang ◽  
O. W. McBride ◽  
M. Sharon ◽  
...  
Keyword(s):  
Chromosomal Localization ◽  
Interleukin 2 ◽  
Chain Gene ◽  
Regulatory Sequences ◽  
Beta Chain ◽  
Interleukin 2 Receptor ◽  
Receptor Beta

scholarly journals The immediate-early gene product Egr-1 regulates the human interleukin-2 receptor beta-chain promoter through noncanonical Egr and Sp1 binding sites.

1997 ◽  
Vol 17 (7) ◽  
pp. 3714-3722 ◽  
Author(s):  
J X Lin ◽  
W J Leonard
Keyword(s):  
Dna Binding ◽  
Binding Site ◽  
Promoter Activity ◽  
Interleukin 2 ◽  
Jurkat Cells ◽  
Early Gene ◽  
Binding Motif ◽  
Beta Chain ◽  
Cell Leukemia Virus Type ◽  
Receptor Beta

The interleukin-2 IL-2 receptor beta-chain (IL-2Rbeta) is an essential component of the receptors for IL-2 and IL-15. Although IL-2Rbeta is constitutively expressed by lymphocytes, its expression can be further induced by a number of stimuli, including phorbol 12-myristate 13-acetate (PMA). We have now characterized factors that bind to an enhancer region located between nucleotides -170 and -139 of the human IL-2Rbeta promoter. Both Sp1 and Sp3 bound to the 5' portion of this region, whereas a PMA-inducible factor (PIF) mainly bound to its 3' portion and bound to the Sp binding motifs as well. In Jurkat T cells, induction of PIF DNA binding activity was rapidly induced, required de novo protein synthesis, and was sustained at a high level for at least 23 h. Interestingly, PIF was constitutively activated in human T-cell leukemia virus type 1-transformed MT-2 cells. In this paper, we demonstrate that PIF is Egr-1 based on its recognition by anti-Egr-1 antisera in gel mobility shift assays, even though the IL-2Rbeta DNA binding motif differed substantially from the canonical Egr-1 binding site. In addition, Egr-1 bound to the Sp binding site. In Jurkat cells, both sites were required for maximal IL-2Rbeta promoter activity, and in HeLaS3 cells, transfection of Egr-1 could drive activity of a reporter construct containing both sites. Moreover, Sp1 and Egr-1 could form a complex with kinetics that correlated with the production of Egr-1 in Jurkat cells upon PMA stimulation. Thus, Sp1 and Egr-1 physically and functionally cooperate to mediate maximal IL-2Rbeta promoter activity.

Abstract 2326: LTX-315, an oncolytic peptide, increases anticancer immunity mediated by CTLA4 blockade in an interleukin-2 receptor beta-chain-dependent manner

2016 ◽  
Author(s):  
Takahiro Yamazaki ◽  
Marie Vetizou ◽  
Camila Flores ◽  
Aurelien Marabelle ◽  
Baldur Sveinbjørnsson ◽  
...  
Keyword(s):  
Interleukin 2 ◽  
Dependent Manner ◽  
Beta Chain ◽  
Interleukin 2 Receptor ◽  
Ctla4 Blockade ◽  
Receptor Beta ◽  
Anticancer Immunity

scholarly journals T-cell receptor beta-chain gene rearrangement and expression during human thymic ontogenesis

Blood ◽  
1992 ◽  
Vol 79 (6) ◽  
pp. 1472-1483 ◽  
Author(s):  
A Bonati ◽  
P Zanelli ◽  
S Ferrari ◽  
A Plebani ◽  
B Starcich ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Chain Gene ◽  
Gene Transcript ◽  
Beta Chain ◽  
Early Stages ◽  
Human Thymus ◽  
Nucleotide Insertions ◽  
Beta Gene

Abstract T-cell receptor (TCR) beta-chain proteins appear early (approximately 15th week of gestation) during human thymic ontogenesis. These beta- chain proteins, which appear before terminal deoxynucleotidyl transferase (TdT), could be an expression of a fully rearranged (V-D- J), incompletely rearranged (D-J), or germline TCR beta-chain gene. The aims of this study, performed from the 15th week onward, were the following: (1) to investigate whether or not TCR beta gene rearranges at an early stage during human thymic ontogenesis; (2) to investigate whether complete presumptive functional (1.3 kb) TCR beta gene transcript is present at these early stages of development, or if incomplete (1 kb) or germ-line (1.1 kb) transcripts are expressed; (3) to examine the phenotype of TCR beta-chain+ cells with two-color fluorescence using monoclonal antibody (MoAb) beta F1 and MoAbs that recognize CD1, CD2, CD3, CD4, CD8, CD5, and CD7 antigens (rabbit anti- calf TdT antiserum was used to detect TdT); and (4) to demonstrate whether or not beta gene N-diversity regions are detectable as early as the 15th week and whether or not N-nucleotide insertions correlate to TdT expression. Fifteen- to 22-week fetal thymuses and pediatric thymuses were investigated. We demonstrated that TCR beta-chain gene rearranged as early as the 15th week in human thymus and that a complete functional TCR beta gene transcript was expressed at these early stages of human development. No other analyses to date have investigated TCR beta gene expression in early human thymus using molecular biology techniques. No significant differences were detectable between phenotypic analysis of fetal and pediatric samples, except for TdT expression, which appeared after the 20th week. Essentially all mCD3+ (OKT3+) cells were beta-chain+ at the different weeks investigated. A significant percentage of CD1+ cells were beta- chain+, and the percentage increased along with the age of development. After the 20th week, we identified three main populations: TdT+, cCD3+, beta F-(early thymic precursors); TdT+, CD1+, beta F1+ (intermediate maturity cortical thymocytes); and TdT-, mCD3+, beta F1++ (mature medullary thymocytes). Given these values, we may consider beta-chain expression an ordered process. beta gene N-nucleotide insertions were correlated to TdT expression, since N-regions increased considerably after the 20th week. A further increase of N-nucleotide insertions was detected from the 22nd week to the 32nd week.

Analysis and Characterization of the Interleukin 2 Receptor alpha and beta Chain Expression inCD4-CD8- Cells

1990 ◽  
Vol 31 (4) ◽  
pp. 485-491
Author(s):  
J. C. GUTIERREZ-RAMOS ◽  
L. PEZZI ◽  
E. LEONARDO ◽  
C. MARTIN ◽  
C. MARTINEZ-A
Keyword(s):  
Interleukin 2 ◽  
Beta Chain ◽  
Cd8 Cells ◽  
Receptor Alpha ◽  
Interleukin 2 Receptor
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