scholarly journals High-throughput screening of tyrosine kinase inhibitor cardiotoxicity with human induced pluripotent stem cells

2017 ◽  
Vol 9 (377) ◽  
pp. eaaf2584 ◽  
Author(s):  
Arun Sharma ◽  
Paul W. Burridge ◽  
Wesley L. McKeithan ◽  
Ricardo Serrano ◽  
Praveen Shukla ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tyrosine Kinase ◽  
Tyrosine Kinase Inhibitor ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
High Throughput Screening ◽  
Kinase Inhibitor ◽  
Induced Pluripotent

Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells

2015 ◽  
Vol 12 (9) ◽  
pp. 885-892 ◽  
Author(s):  
Daniel Paull ◽  
Ana Sevilla ◽  
Hongyan Zhou ◽  
Aana Kim Hahn ◽  
Hesed Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals Multiplex High-Throughput Targeted Proteomic Assay To Identify Induced Pluripotent Stem Cells

2017 ◽  
Vol 89 (4) ◽  
pp. 2440-2448 ◽  
Author(s):  
Anna Baud ◽  
Frank Wessely ◽  
Francesca Mazzacuva ◽  
James McCormick ◽  
Stephane Camuzeaux ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals High-throughput propagation of human prostate tissue from induced-pluripotent stem cells

2019 ◽  
Author(s):  
AC Hepburn ◽  
EL Curry ◽  
M Moad ◽  
RE Steele ◽  
OE Franco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
Neuroendocrine Cells ◽  
Prostate Tissue ◽  
Urogenital Sinus ◽  
Human Prostate ◽  
Human Prostate Tissue ◽  
Induced Pluripotent

AbstractPrimary culture of human prostate organoids is slow, inefficient and laborious. To overcome this, we demonstrate a new high-throughput model where rapidly proliferating and easily handled induced pluripotent stem cells, for the first time, enable generation of human prostate tissue in vivo and in vitro. Using a co-culture technique with urogenital sinus mesenchyme, we recapitulated the in situ prostate histology, including the stromal compartment and the full spectrum of epithelial differentiation. This approach overcomes major limitations in primary cultures of human prostate stem, luminal and neuroendocrine cells, as well as the stromal microenvironment. These models provide new opportunities to study prostate development, homeostasis and disease.

scholarly journals Rapid and efficient generation of oligodendrocytes from human induced pluripotent stem cells using transcription factors

2017 ◽  
Vol 114 (11) ◽  
pp. E2243-E2252 ◽  
Author(s):  
Marc Ehrlich ◽  
Sabah Mozafari ◽  
Michael Glatza ◽  
Laura Starost ◽  
Sergiy Velychko ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
High Throughput Screening ◽  
Neural Progenitor Cells ◽  
Disease Modeling ◽  
Global Gene Expression ◽  
Induced Pluripotent

Rapid and efficient protocols to generate oligodendrocytes (OL) from human induced pluripotent stem cells (iPSC) are currently lacking, but may be a key technology to understand the biology of myelin diseases and to develop treatments for such disorders. Here, we demonstrate that the induction of three transcription factors (SOX10, OLIG2, NKX6.2) in iPSC-derived neural progenitor cells is sufficient to rapidly generate O4+ OL with an efficiency of up to 70% in 28 d and a global gene-expression profile comparable to primary human OL. We further demonstrate that iPSC-derived OL disperse and myelinate the CNS of Mbpshi/shiRag−/− mice during development and after demyelination, are suitable for in vitro myelination assays, disease modeling, and screening of pharmacological compounds potentially promoting oligodendroglial differentiation. Thus, the strategy presented here to generate OL from iPSC may facilitate the studying of human myelin diseases and the development of high-throughput screening platforms for drug discovery.

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