scholarly journals Identification of NDM-1 in a Putatively Novel Acinetobacter Species (“NB14”) Closely Related to Acinetobacter pittii

2015 ◽  
Vol 59 (10) ◽  
pp. 6657-6660 ◽  
Author(s):  
Paula Espinal ◽  
Noraida Mosqueda ◽  
Murat Telli ◽  
Tanny van der Reijden ◽  
Dora Rolo ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Restriction Analysis ◽  
Conjugative Plasmid ◽  
Acinetobacter Species ◽  
Content Type ◽  
16S Ribosomal Dna ◽  
Acinetobacter Pittii ◽  
Dna Restriction ◽  
Molecular Sequencing

ABSTRACTIn this study, we describe the molecular characterization of a plasmid-locatedblaNDM-1harbored by anAcinetobacterclinical isolate recovered from a patient in Turkey that putatively constitutes a novelAcinetobacterspecies, as shown by its distinct ARDRA (amplified 16S ribosomal DNA restriction analysis) profile and molecular sequencing techniques.blaNDM-1was carried by a conjugative plasmid widespread among non-baumannii Acinetobacterisolates, suggesting its potential for dissemination before reaching more clinically relevantAcinetobacterspecies.

Photosynthetic Bradyrhizobia from Aeschynomene spp. Are Specific to Stem-Nodulated Species and Form a Separate 16S Ribosomal DNA Restriction Fragment Length Polymorphism Group

1999 ◽  
Vol 65 (7) ◽  
pp. 3084-3094 ◽  
Author(s):  
Flore Molouba ◽  
Jean Lorquin ◽  
Anne Willems ◽  
Bart Hoste ◽  
Eric Giraud ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Rhodobacter Capsulatus ◽  
Restriction Analysis ◽  
Dna Amplification ◽  
Rrna Gene ◽  
Gene Probe ◽  
16S Ribosomal Dna ◽  
Photosynthetic Genes ◽  
Dna Restriction ◽  
Layer Chromatography

ABSTRACT We obtained nine bacterial isolates from root or collar nodules of the non-stem-nodulated Aeschynomene species A. elaphroxylon, A. uniflora, or A. schimperi and 69 root or stem nodule isolates from the stem-nodulated Aeschynomene species A. afraspera, A. ciliata, A. indica,A. nilotica, A. sensitiva, and A. tambacoundensis from various places in Senegal. These isolates, together with 45 previous isolates from variousAeschynomene species, were studied for host-specific nodulation within the genus Aeschynomene, also revisiting cross-inoculation groups described previously by D. Alazard (Appl. Environ. Microbiol. 50:732–734, 1985). The whole collection ofAeschynomene nodule isolates was screened for synthesis of photosynthetic pigments by spectrometry, high-pressure liquid chromatography, and thin-layer chromatography analyses. The presence ofpuf genes in photosyntheticAeschynomene isolates was evidenced both by Southern hybridization with a Rhodobacter capsulatus photosynthetic gene probe and by DNA amplification with primers defined from photosynthetic genes. In addition, amplified 16S ribosomal DNA restriction analysis was performed on 45 Aeschynomeneisolates, including strain BTAi1, and 19 reference strains fromBradyrhizobium japonicum, Bradyrhizobium elkanii, and other Bradyrhizobium sp. strains of uncertain taxonomic positions. The 16S rRNA gene sequence of the photosynthetic strain ORS278 (LMG 12187) was determined and compared to sequences from databases. Our main conclusion is that photosynthetic Aeschynomene nodule isolates share the ability to nodulate particular stem-nodulated species and form a separate subbranch on the Bradyrhizobium rRNA lineage, distinct from B. japonicum and B. elkanii.

Amplified 16S Ribosomal DNA Restriction Analysis for Identification of Avibacterium paragallinarum

2008 ◽  
Vol 52 (1) ◽  
pp. 54-58 ◽  
Author(s):  
Alfredo Mendoza-Espinoza ◽  
Ysabel Koga ◽  
Amparo I. Zavaleta
Keyword(s):  
Ribosomal Dna ◽  
Restriction Analysis ◽  
16S Ribosomal Dna ◽  
Avibacterium Paragallinarum ◽  
Dna Restriction

scholarly journals 16S Ribosomal DNA Characterization of Nitrogen-Fixing Bacteria Isolated from Banana (Musa spp.) and Pineapple (Ananas comosus (L.) Merril)

2001 ◽  
Vol 67 (5) ◽  
pp. 2375-2379 ◽  
Author(s):  
Leonardo Magalhães Cruz ◽  
Emanuel Maltempi de Souza ◽  
Olmar Baler Weber ◽  
José Ivo Baldani ◽  
Johanna Döbereiner ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Restriction Analysis ◽  
Ananas Comosus ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
16S Rrna Sequence ◽  
16S Ribosomal Dna ◽  
Close Proximity ◽  
16S Rrna Sequence Analysis ◽  
Dna Restriction

ABSTRACT Nitrogen-fixing bacteria isolated from banana (Musaspp.) and pineapple (Ananas comosus (L.) Merril) were characterized by amplified 16S ribosomal DNA restriction analysis and 16S rRNA sequence analysis. Herbaspirillum seropedicae, Herbaspirillum rubrisubalbicans,Burkholderia brasilensis, and Burkholderia tropicalis were identified. Eight other types were placed in close proximity to these genera and other alpha and betaProteobacteria.

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