scholarly journals Identification of a Novel 6′-N-Aminoglycoside Acetyltransferase, AAC(6′)-Iak, from a Multidrug-Resistant Clinical Isolate of Stenotrophomonas maltophilia

2014 ◽  
Vol 58 (10) ◽  
pp. 6324-6327 ◽  
Author(s):  
Tatsuya Tada ◽  
Tohru Miyoshi-Akiyama ◽  
Rajan K. Dahal ◽  
Shyam K. Mishra ◽  
Kayo Shimada ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Clinical Isolate ◽  
Stenotrophomonas Maltophilia ◽  
Multidrug Resistant ◽  
Content Type ◽  
Encoding Gene ◽  
Layer Chromatography

ABSTRACTStenotrophomonas maltophiliaIOMTU250 has a novel 6′-N-aminoglycoside acetyltransferase-encoding gene,aac(6′)-Iak. The encoded protein, AAC(6′)-Iak, consists of 153 amino acids and has 86.3% identity to AAC(6′)-Iz.Escherichia colitransformed with a plasmid containingaac(6′)-Iakexhibited decreased susceptibility to arbekacin, dibekacin, neomycin, netilmicin, sisomicin, and tobramycin. Thin-layer chromatography showed that AAC(6′)-Iak acetylated amikacin, arbekacin, dibekacin, isepamicin, kanamycin, neomycin, netilmicin, sisomicin, and tobramycin but not apramycin, gentamicin, or lividomycin.

THIN-LAYER CHROMATOGRAPHY OF AMINO ACIDS: A REVIEW

2012 ◽  
Vol 35 (11) ◽  
pp. 1497-1516 ◽  
Author(s):  
S. A. Bhawani ◽  
M. N. Mohamad Ibrahim ◽  
O. Sulaiman ◽  
R. Hashim ◽  
A. Mohammad ◽  
...  
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

Derivative Spectroscopy in Conjunction with Thin Layer Chromatography Applied for Determination of Mixtures of Amino Acids in Baby Food.

1997 ◽  
Vol 30 (4) ◽  
pp. 649-660 ◽  
Author(s):  
A. Makriyannis ◽  
A. A. El. Kheir ◽  
M. M. Ayad ◽  
W. S. Hassan ◽  
M. M.F. Metwally
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Baby Food ◽  
Derivative Spectroscopy ◽  
Layer Chromatography

Identification and Quantitation of Panthenol and Pantothenic Acid in Pharmaceutical Preparations by Thin-Layer Chromatography and Densitometry

1992 ◽  
Vol 75 (5) ◽  
pp. 898-901 ◽  
Author(s):  
Saurendra S Nag ◽  
Saroj K Das
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Benzyl Alcohol ◽  
Silica Gel ◽  
Pantothenic Acid ◽  
Pharmaceutical Preparations ◽  
Ninhydrin Reaction ◽  
Layer Chromatography

Abstract A rapid, simple, and specific thin-layer chromatographic (TLC) method has been developed for the estimation of panthenol and pantothenic acid in pharmaceutical preparations containing other vitamins, amino acids, syrups, enzymes, etc. The vitamin is extracted with ethanol (tablets and capsules) or benzyl alcohol (liquid oral preparations) and isolated from other ingredients by TLC on silica gel with isopropanol-water (85 + 15, v/v) as developing solvent. β-Alanine (pantothenate) or β-alanol (panthenol) is liberated by heating for 20 min at 160°C. The liberated amines are visualized by ninhydrin reaction and estimated by spectrodensitometry at 490 nm. Recoveries for panthenol and pantothenic acid were 99.8 ± 2.25% and 100.2 ± 1.7%, respectively.

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