scholarly journals Comparative activity of (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine and 9-(1,3-dihydroxy-2-propoxymethyl)guanine against rat cytomegalovirus infection in vitro and in vivo.

1991 ◽  
Vol 35 (11) ◽  
pp. 2262-2266 ◽  
Author(s):  
F S Stals ◽  
E de Clercq ◽  
C A Bruggeman
Keyword(s):  
Cytomegalovirus Infection ◽  
Comparative Activity

Comparative activity of anticestode drugs—praziquantel, niclosamide and Compound 77–6, against Hymenolepis nana

1983 ◽  
Vol 57 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Suman Gupta ◽  
J. C. Katiyar
Keyword(s):  
Drug Concentration ◽  
Hymenolepis Nana ◽  
Comparative Activity

AbstractThe activity, in terms of speed of action, of three anticestode drugs against Hymenolepis nana, both in vivo and in vitro, was investigated. Praziquantel was most effective in vivo, but had little action on adult worms and cysticercoids in vitro. Niclosamide, the least effective in vivo, was highly toxic in vitro. Compound 77–6 killed adult worms and cysticercoids in vitro in 10 min and 15 min respectively at 1000 μg/ml of drug concentration, but its in viro effect was intermediate between that of praziquantel and niclosamide.

Replication of murine cytomegalovirus in lung macrophages: effect of phagocytosis of bacteria

1980 ◽  
Vol 29 (3) ◽  
pp. 1152-1159
Author(s):  
J D Shanley ◽  
E L Pesanti
Keyword(s):  
Cytomegalovirus Infection ◽  
Peritoneal Macrophages ◽  
Murine Cytomegalovirus ◽  
Infectious Agents ◽  
Direct Inhibition ◽  
Viral Antigens ◽  
Indirect Immunofluorescence Microscopy ◽  
Infected Cells

Murine cytomegalovirus was found to replicate in lung and peritoneal macrophages of both CF-1 and BALB/c mice in vitro. Cytopathic changes typical of cytomegalovirus infection, including intranuclear inclusions, developed within the infected cells and eventually resulted in death of infected macrophages. Viral antigens were demonstrable by indirect immunofluorescence microscopy, and morphologically typical herpesvirus particles were observed in both nuclei and cytoplasm of murine cytomegalovirus-infected macrophages. Within 24 h after infection, at which time there was expression of viral antigens but no marcophage death, murine cytomegalovirus-infected macrophages demonstrated marked inhibition of phagocytosis of Staphylococcus aureus. Direct inhibition of macrophage function by cytomegalovirus infection in vivo could impair pulmonary defenses and may account in part for the frequent association of cytomegalovirus infection with other infectious agents.

scholarly journals Antiviral effect of oxetanocin G against guinea pig cytomegalovirus infection in vitro and in vivo.

1990 ◽  
Vol 43 (12) ◽  
pp. 1573-1578 ◽  
Author(s):  
NAOHIKO YAMAMOTO ◽  
YOSHINARI YAMADA ◽  
TOHRU DAIKOKU ◽  
YUKIHIRO NISHIYAMA ◽  
YOSHIHIRO TSUTSUI ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Cytomegalovirus Infection ◽  
Antiviral Effect ◽  
Guinea Pig Cytomegalovirus

Biochemical Activities of Propolis-Extracts III. Inhibition of Dihydrofolate Reductase

1994 ◽  
Vol 49 (1-2) ◽  
pp. 39-43 ◽  
Author(s):  
Elke Strehl ◽  
Regina Volpert ◽  
Erich F. Elstner
Keyword(s):  
Dihydrofolate Reductase ◽  
Antiinflammatory Drugs ◽  
Propolis Extract ◽  
Biochemical Model ◽  
Biochemical Mechanisms ◽  
Comparative Activity ◽  
Model Reactions

Ethanolic and aqueous extracts of the natural compound PROPOLIS indicate substantial antiinflammatory functions as well as antibiotic activities in vitro and in vivo. The exact mode of physiological or biochemical mechanisms responsible for the medical effects, however, is all but clear. The standardization on the basis of quantitative determination of prominent components of these extracts have been substituted recently by simple biochemical model reactions including photodynamic properties. In this communication we report on the inhibitory activity of an aqueous extract of propolis on the enzyme dihydrofolate reductase. This activity may at least partially be due to the content of caffeic acid, as revealed by HPLC chromatography and comparative activity tests of representative ingredients of the propolis extract. This result may explain some of the protective functions of propolis, similar to those shown for several “non-steroidal antiinflammatory drugs”, NSAIDs.

Immediate Loss of Cell-Mediated Immunity to Murine Cytomegalovirus upon Treatment with Immunosuppresive Agents

1980 ◽  
Vol 30 (3) ◽  
pp. 700-708
Author(s):  
Donald M. Mattsson ◽  
Richard J. Howard ◽  
Henry H. Balfour
Keyword(s):  
Cytomegalovirus Infection ◽  
Proliferative Response ◽  
Murine Cytomegalovirus ◽  
Cell Mediated Immunity ◽  
Blast Transformation ◽  
Specific Cell ◽  
Antilymphocyte Globulin ◽  
Prednisolone Treatment

Splenic lymphocytes from cytomegalovirus-infected mice lost their in vitro proliferative responses to cytomegalovirus antigen within 3 h after in vivo treatment with antilymphocyte globulin and prednisolone. The response was inhibited when the agents were administered separately or together, and inhibition persisted through a 2-week course of immunosuppression. Circulating specific antibodies were depressed by multiple injections of antilymphocyte globulin alone or with prednisolone, but not by prednisolone alone. Mitogen-induced blast transformation was immediately depressed by immunosuppression with both agents. Although the response to lipopolysaccharide returned briefly, it declined with continuing treatment. Cytomegalovirus infection augmented the depressive effect of immunosuppression on the lipopolysaccharide proliferative response. Prednisolone treatment of infected animals did not affect the concanavalin A response, and lipopolysaccharide stimulation decreased more slowly and to a lesser extent than it did in mice treated with antilymphocyte globulin or both agents. Loss of specific cell-mediated immunity and simultaneous depression of humoral immunity indicated that immunosuppression immediately created an inability to respond to an active cytomegalovirus infection.

scholarly journals The D-form of a novel heparan binding peptide decreases cytomegalovirus infection in vivo and in vitro

2016 ◽  
Vol 135 ◽  
pp. 15-23 ◽  
Author(s):  
Elisabeth A. Pitt ◽  
Pranay Dogra ◽  
Ravi S. Patel ◽  
Angela Williams ◽  
Jonathan S. Wall ◽  
...  
Keyword(s):  
Cytomegalovirus Infection ◽  
Binding Peptide

Inhibition of cytomegalovirus infection by lactoferrin in vitro and in vivo

2004 ◽  
Vol 63 (3) ◽  
pp. 197-208 ◽  
Author(s):  
L BELJAARS ◽  
B VANDERSTRATE ◽  
H BAKKER ◽  
C REKERSMIT ◽  
A VANLOENENWEEMAES ◽  
...  
Keyword(s):  
Cytomegalovirus Infection

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

1982 ◽  
Vol 40 ◽  
pp. 210-211
Author(s):  
M.J. Murphy ◽  
R.R. Price ◽  
J.C. Sloman
Keyword(s):  
Cultured Cells ◽  
Human Tumor ◽  
Cell Type ◽  
Tumor Mass ◽  
Initial Cell ◽  
Cloning Assay ◽  
Human Tumor Cloning ◽  
The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

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