Chromosome- and Plasmid-Encoded β-Lactamases in Capnocytophaga spp

ABSTRACT Chromosome- and plasmid-encoded CfxA2 and CfxA3 β-lactamases were detected in Capnocytophaga spp. from oral sources in France, Norway, and the United States. Unidentified chromosome-encoded β-lactamases were present in Capnocytophaga sputigena. Nucleotide sequence analysis of the CfxA3-encoding plasmid from C. ochracea revealed an unreported insertion sequence (ISCoc1) upstream of the cfxA gene.

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Nucleotide sequence analysis of the precore region in patients with fulminant hepatitis B in the United States

Gastroenterology ◽

10.1016/0016-5085(93)90964-e ◽

1993 ◽

Vol 105 (4) ◽

pp. 1173-1178 ◽

Cited By ~ 66

Author(s):

Tomasz Laskus ◽

David H. Persing ◽

Marek J. Nowicki ◽

James W. Mosley ◽

Jorge Rakela

Keyword(s):

United States ◽

Sequence Analysis ◽

Nucleotide Sequence ◽

Hepatitis B ◽

Fulminant Hepatitis ◽

The United States ◽

Nucleotide Sequence Analysis ◽

Precore Region

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Human papillomavirus type 16 variant lineages in United States populations characterized by nucleotide sequence analysis of the E6, L2, and L1 coding segments.

Journal of Virology ◽

10.1128/jvi.69.12.7743-7753.1995 ◽

1995 ◽

Vol 69 (12) ◽

pp. 7743-7753 ◽

Cited By ~ 148

Author(s):

T Yamada ◽

C M Wheeler ◽

A L Halpern ◽

A C Stewart ◽

A Hildesheim ◽

...

Keyword(s):

United States ◽

Human Papillomavirus ◽

Sequence Analysis ◽

Nucleotide Sequence ◽

Nucleotide Sequence Analysis ◽

Human Papillomavirus Type 16

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First Report of Alternanthera mosaic virus Infection in Angelonia in the United States

Plant Disease ◽

10.1094/pdis-92-10-1473b ◽

2008 ◽

Vol 92 (10) ◽

pp. 1473-1473 ◽

Cited By ~ 7

Author(s):

B. E. Lockhart ◽

M. L. Daughtrey

Keyword(s):

United States ◽

Nucleotide Sequence ◽

Mosaic Virus ◽

Genomic Sequence ◽

Nutrient Deficiency ◽

Leaf Tissue ◽

The United States ◽

Nucleotide Sequence Analysis ◽

Degenerate Primers ◽

First Report

Stunting, chlorosis, and light yellow mottling resembling symptoms of nutrient deficiency were observed in angelonia (Angelonia angustifolia) in commercial production in New York. Numerous, filamentous particles 520 to 540 nm long and spherical virus particles 30 nm in diameter were observed by transmission electron microscopy (TEM) in negatively stained partially purified extracts of symptomatic Angelonia leaf tissue. Two viruses, the filamentous potexvirus Alternanthera mosaic virus (AltMV) and the spherical carmovirus Angelonia flower break virus (AnFBV) were subsequently identified on the basis of nucleotide sequence analysis of amplicons generated by reverse transcription (RT)-PCR using total RNA isolated from infected leaf tissue. A 584-bp portion of the replicase-encoding region of the AltMV genome was obtained with the degenerate primers Potex 2RC (5′-AGC ATR GNN SCR TCY TG-3′) and Potex 5 (5′-CAY CAR CAR GCM AAR GAT GA-3′) (3). Forward (AnFBV CP 1F-5′-AGC CTG GCA ATC TGC GTA CTG ATA-3′) and reverse (AnFBV CP 1R-5′-AAT ACC GCC CTC CTG TTT GGA AGT-3′) primers based on the published AnFBV genomic sequence (GenBank Accession No. NC_007733) were used to amplify a portion of the viral coat protein (CP) gene. The nucleotide sequence of the amplicon generated using the potexvirus-specific primers (GenBank Accession No. EU679362) was 99% identical to the published AltMV (GenBank Accession No. NC_007731) sequence and the nucleotide sequence of the amplicon obtained using the AnFBV CP primers was 99% identical to the published AnFBV genomic sequence (GenBank Accession No. EU679363). AnFBV occurs widely in angelonia (1) and AltMV has been identified in phlox (2). These data confirm the presence of AltMV and AnFBV in diseased angelonia plants showing stunting and nutrient deficiency-like symptoms and substantiates, to our knowledge, this first report of AltMV in angelonia in the United States. References: (1) S. Adkins et al. Phytopathology 96:460, 2006. (2) J. Hammond et al. Arch. Virol. 151:477, 2006. (3) R. A. A. van der Vlugt and M. Berendeson. Eur. J. Plant Pathol. 108:367, 2002.

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Nucleotide sequence analysis of two 5-nitroimidazole resistance determinants from Bacteroides strains and of a new insertion sequence upstream of the two genes.

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.38.5.1047 ◽

1994 ◽

Vol 38 (5) ◽

pp. 1047-1051 ◽

Cited By ~ 49

Author(s):

A Haggoud ◽

G Reysset ◽

H Azeddoug ◽

M Sebald

Keyword(s):

Sequence Analysis ◽

Nucleotide Sequence ◽

Insertion Sequence ◽

Nucleotide Sequence Analysis ◽

Resistance Determinants

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Characterization of Mycobacterium avium clinical isolates in Japan using subspecies-specific insertion sequences, and identification of a new insertion sequence, ISMav6

Journal of Medical Microbiology ◽

10.1099/jmm.0.008623-0 ◽

2009 ◽

Vol 58 (7) ◽

pp. 945-950 ◽

Cited By ~ 25

Author(s):

Kazuya Ichikawa ◽

Tetsuya Yagi ◽

Makoto Moriyama ◽

Takayuki Inagaki ◽

Taku Nakagawa ◽

...

Keyword(s):

Sequence Analysis ◽

Nucleotide Sequence ◽

Mycobacterium Avium ◽

Insertion Sequence ◽

Point Mutations ◽

Clinical Isolates ◽

Nucleotide Sequence Analysis ◽

Nucleotide Sequencing ◽

Insertion Sequences ◽

Pcr Products

Clinical isolates of Mycobacterium avium (n=81) from patients with pulmonary infections who were HIV-negative and isolates (n=33) from HIV-positive patients were subjected to genetic analysis by PCR detection of three M. avium-specific insertion sequences (IS901, IS1245 and IS1311), and nucleotide sequencing of the heat-shock protein 65 (hsp65) gene. All clinical isolates were identified as ‘M. avium subspecies hominissuis’ by sequence analysis of hsp65. Compared with clinical isolates of M. avium reported elsewhere, IS1245 was found less frequently in Japanese isolates (96/114 isolates, 84 %) and IS901 was detected more frequently (76/114 isolates, 67 %). One isolate was found to lack IS1311, which has not been reported previously for ‘M. avium subsp. hominissuis’. Nucleotide sequence analysis of the PCR products for IS901 revealed that all clinical isolates had the same new insertion sequence, designated ISMav6, which had 60 point mutations compared with the nucleotide sequence of the original IS901. These results suggest that ‘M. avium subsp. hominissuis’ with ISMav6 is prevalent in Japan. ISMav6 may have implications for the virulence of M. avium and contribute to an increase of M. avium infections in this country.

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