scholarly journals Random Mutagenesis of Clostridium cellulolyticum by Using a Tn1545 Derivative

2010 ◽  
Vol 76 (13) ◽  
pp. 4546-4549 ◽  
Author(s):  
Jean-Charles Blouzard ◽  
Odile Valette ◽  
Chantal Tardif ◽  
Pascale de Philip

ABSTRACT Further understanding of the plant cell wall degradation system of Clostridium cellulolyticum and the possibility of metabolic engineering in this species highlight the need for a means of random mutagenesis. Here, we report the construction of a Tn1545-derived delivery tool which allows monocopy random insertion within the genome.

2014 ◽  
Vol 111 (17) ◽  
pp. 6287-6292 ◽  
Author(s):  
J. W. Agger ◽  
T. Isaksen ◽  
A. Varnai ◽  
S. Vidal-Melgosa ◽  
W. G. T. Willats ◽  
...  

1990 ◽  
Vol 33 (3) ◽  
pp. 345-351 ◽  
Author(s):  
William S. Borneman ◽  
Roy D. Hartley ◽  
W. Herbert Morrison ◽  
Danny E. Akin ◽  
Lars G. Ljungdahl

2009 ◽  
Vol 20 (3) ◽  
pp. 330-338 ◽  
Author(s):  
Hui Wei ◽  
Qi Xu ◽  
Larry E Taylor ◽  
John O Baker ◽  
Melvin P Tucker ◽  
...  

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