scholarly journals Derivation of High Enterotoxin B-Producing Mutants of Staphylococcus aureus from the Parent Strains

1975 ◽  
Vol 30 (2) ◽  
pp. 271-275 ◽  
Author(s):  
Robert A. Altenbern
Keyword(s):  
Staphylococcus Aureus ◽  
Enterotoxin B

Enterotoxin B formation by fermentation mutants of Staphylococcus aureus

1976 ◽  
Vol 22 (2) ◽  
pp. 182-188
Author(s):  
Robert A. Altenbern
Keyword(s):  
Staphylococcus Aureus ◽  
Point Mutations ◽  
Type A ◽  
Enterotoxin B

An appreciable fraction of carbohydrate-negative (car) mutants of Staphylococcus aureus strains ATCC 14458,778, and S-6 exhibit increased enterotoxin B (SEB) production. In addition, some lac and mtl mutants of these strains also display enhanced SEB formation. All such mutants appear to be point mutations. Mutagen-induced reversions of high SEB producing car, mtl, or lac mutants yield varying amounts of SEB and some clones seem to be restored to the characteristics of the parent type. A few sequentially isolated lac, mtl double mutants of strain 778 elaborate much more or much less SEB than either the lac or the mtl single mutants.

scholarly journals Effect of Shaking Speed on the Secretion of Enterotoxin B by Staphylococcus aureus

1972 ◽  
Vol 24 (4) ◽  
pp. 561-566 ◽  
Author(s):  
Glynn G. Dietrich ◽  
Robert J. Watson ◽  
Gerald J. Silverman
Keyword(s):  
Staphylococcus Aureus ◽  
Enterotoxin B

scholarly journals Staphylococcus aureus Enterotoxin B Release (Excretion) Under Controlled Conditions of Fermentation

1973 ◽  
Vol 25 (5) ◽  
pp. 770-773
Author(s):  
Joseph F. Metzger ◽  
Anna D. Johnson ◽  
William S. Collins ◽  
Virginia McGann
Keyword(s):  
Staphylococcus Aureus ◽  
Controlled Conditions ◽  
Enterotoxin B ◽  
Staphylococcus Aureus Enterotoxin B

Membrane mutations and production of enterotoxin B and alpha hemolysin in Staphylococcus aureus

1975 ◽  
Vol 21 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Robert A. Altenbern
Keyword(s):  
Staphylococcus Aureus ◽  
Aureus Strain ◽  
Type B ◽  
Alpha Hemolysin ◽  
Osmotic Stability ◽  
Enterotoxin B

Staphylococcus aureus strain S-6, which produces enterotoxin type B (SEB), and strain 10-275, a high toxin-producing mutant derived from S-6, display pronounced differences in dye sensitivity, osmotic stability, and bacitracin sensitivity. Such characteristics are consistent with the concept that strain 10-275 is a membrane mutant of strain S-6. Some membrane mutants of S. aureus strain 14458 exhibit about two- to three-fold increases in SEB production whereas other membrane mutants show about twofold increases in α-hemolysin production. It is suggested that specific and independent membrane mutations control the secretory processes resulting in the extracellular elaboration of these exoproteins.

Enterotoxin B production by Staphylococcus aureus under controlled fatty acid nutrition induced by cerulenin

1977 ◽  
Vol 23 (9) ◽  
pp. 1145-1150 ◽  
Author(s):  
Robert A. Altenbern
Keyword(s):  
Fatty Acids ◽  
Staphylococcus Aureus ◽  
Fatty Acid ◽  
Membrane Fluidity ◽  
Unsaturated Fatty Acid ◽  
Unsaturated Fatty Acids ◽  
Basal Medium ◽  
Aureus Strain ◽  
Enterotoxin B ◽  
Fatty Acid Supplement

Cells of Staphylococcus aureus, strain S-6, can grow in the presence of 100 μg of cerulenin/ml if the basal medium is supplemented with certain saturated or unsaturated fatty acids. The production of enterotoxin B (SEB) is markedly influenced by both the ratio of saturated to unsaturated fatty acid and by the melting point of the unsaturated fatty acid supplement. The results presented suggest that a certain degree of membrane fluidity promotes maximum SEB production and that greater or lesser degrees of membrane fluidity prohibit substantial SEB formation but fail to affect final growth density.

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