Inability of Pseudomonas stutzeri Denitrification Mutants with the Phenotype of Pseudomonas aeruginosa to Grow in Nitrous Oxide

1986 ◽  
Vol 51 (3) ◽  
pp. 671-671
1991 ◽  
Vol 43 (2-3) ◽  
pp. 181
Author(s):  
Jacqui A. Farrar ◽  
Andrew J. Thomson ◽  
Myles R. Cheesman ◽  
David M. Dooley ◽  
Walter G. Zumft

Author(s):  
Cristiane Güths da Silva De Freitas ◽  
Keli Jaqueline Staudt ◽  
Kelly Helena Khün ◽  
Izabel Almeida Alves ◽  
Maria Cristina Meneghete

<p><strong>Introdução:</strong> Os hospitais constituem importante fonte de infecções, pois, abrigam uma vasta gama de microrganismos, principalmente bactérias. A limpeza e desinfecção de superfícies em ambientes hospitalares são subsídios elementares e eficazes como medidas de controle para romper a cadeia epidemiológica das infecções. <strong>Objetivo</strong>: Verificar a prevalência de microrganismos em bandejas utilizadas pela enfermagem para a administração de medicamentos em ambiente hospitalar, através do crescimento dos mesmos por técnicas microbiológicas. <strong>Métodos:</strong> Trata-se de uma pesquisa do tipo transversal, de prevalência, com abordagem quantitativa realizada em um hospital de médio porte da região Noroeste do Estado do Rio Grande do Sul, no segundo semestre de 2015. A coleta das amostras se deu por meio da técnica de swab, que consistiu em deslizar um swab na superfície das bandejas utilizadas para a administração dos medicamentos. Para a análise dos dados foi realizada a pesquisa microbiológica e utilizada a estatística descritiva mediante a distribuição da frequência. <strong>Resultados:</strong> Os microrganismos isolados foram: <em>Staphylococcus </em>coagulase negativa, <em>Acinetobacter baumanni</em>,  <em>Enterobacter agglomerans</em>, <em>Klebsiella oxytoca, Klebsiella ozaenae</em>, <em>Staphylococcus aureus</em>, <em>Acinetobacter lwoffii, Pseudomonas stutzeri, </em><em>Pseudomonas aeruginosa</em>. <strong>Conclusão:</strong> Demonstrando a importância de se realizar os processos de higienização correta das mãos, dos materiais, dos utensílios, dentre outras, como forma possível de reduzir a transferência de patógenos entre profissionais, pacientes e ambiente.</p>


Diversity ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 289
Author(s):  
Marios Nikolaidis ◽  
Dimitris Mossialos ◽  
Stephen G. Oliver ◽  
Grigorios D. Amoutzias

The Pseudomonas genus includes many species living in diverse environments and hosts. It is important to understand which are the major evolutionary groups and what are the genomic/proteomic components they have in common or are unique. Towards this goal, we analyzed 494 complete Pseudomonas proteomes and identified 297 core-orthologues. The subsequent phylogenomic analysis revealed two well-defined species (Pseudomonas aeruginosa and Pseudomonas chlororaphis) and four wider phylogenetic groups (Pseudomonas fluorescens, Pseudomonas stutzeri, Pseudomonas syringae, Pseudomonas putida) with a sufficient number of proteomes. As expected, the genus-level core proteome was highly enriched for proteins involved in metabolism, translation, and transcription. In addition, between 39–70% of the core proteins in each group had a significant presence in each of all the other groups. Group-specific core proteins were also identified, with P. aeruginosa having the highest number of these and P. fluorescens having none. We identified several P. aeruginosa-specific core proteins (such as CntL, CntM, PlcB, Acp1, MucE, SrfA, Tse1, Tsi2, Tse3, and EsrC) that are known to play an important role in its pathogenicity. Finally, a holin family bacteriocin and a mitomycin-like biosynthetic protein were found to be core-specific for P. cholororaphis and we hypothesize that these proteins may confer a competitive advantage against other root-colonizers.


1994 ◽  
Vol 176 (9) ◽  
pp. 2532-2542 ◽  
Author(s):  
D H Kwon ◽  
C D Lu ◽  
D A Walthall ◽  
T M Brown ◽  
J E Houghton ◽  
...  

1988 ◽  
Vol 252 (2) ◽  
pp. 427-435 ◽  
Author(s):  
G W Pettigrew ◽  
K R Brown

Cytochrome c4 was isolated from cells of Pseudomonas aeruginosa, Pseudomonas stutzeri and Azotobacter vinelandii. The dihaem nature, Mr of approx. 20,000 and ferrohaem spectra in the region of the alpha- and beta-peaks define this family of cytochromes c. The behaviour of the holocytochromes in SDS was atypical, but removal of the haem groups resulted in a normal migration. In all three organisms most of the cytochrome c4 was tightly bound to the membrane, but some free cytochrome was detected. The membrane-attached cytochrome could be extracted with butanol, and this solubilized form was then indistinguishable in properties from the free form. Denitrifying rather than aerobic growth conditions hardly affected the total cytochrome c4 in the two pseudomonads, but there was slightly more free form and less membrane-attached form in denitrifying growth. The nature of the attachment of cytochrome c4 to the membrane is discussed and a model is proposed for the process of solubilization.


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