scholarly journals Fully automatic determination of soil bacterium numbers, cell volumes, and frequencies of dividing cells by confocal laser scanning microscopy and image analysis.

1995 ◽  
Vol 61 (3) ◽  
pp. 926-936 ◽  
Author(s):  
J Bloem ◽  
M Veninga ◽  
J Shepherd
Keyword(s):  
Image Analysis ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Automatic Determination ◽  
Dividing Cells ◽  
Fully Automatic ◽  
Cell Volumes

scholarly journals Autofluorescence as a Tool for Structural Analysis of Biofilms Formed by Nonpigmented Rapidly Growing Mycobacteria

2012 ◽  
Vol 79 (3) ◽  
pp. 1065-1067 ◽  
Author(s):  
María-Carmen Muñoz-Egea ◽  
María García-Pedrazuela ◽  
Ignacio Mahillo ◽  
María Jesús García ◽  
Jaime Esteban
Keyword(s):  
Image Analysis ◽  
Structural Analysis ◽  
Cell Viability ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Rapidly Growing Mycobacteria

ABSTRACTThe structure of biofilms formed by seven nonpigmented rapidly growing mycobacteria, including saprophytes and opportunistic species, was analyzed. Analysis included amount of covered surface, thickness, cell viability, and presence of intrinsic autofluorescence at different times using confocal laser scanning microscopy and image analysis. Autofluorescence was detected inside and outside cells of all mycobacteria.

A Simple and Rapid Staining Technique for Sex Determination of Trichinella Larvae Parasites by Confocal Laser Scanning Microscopy

2019 ◽  
Vol 25 (6) ◽  
pp. 1491-1497 ◽  
Author(s):  
Inese Gavarane ◽  
Elena Kirilova ◽  
Ilze Rubeniņa ◽  
Ligita Mežaraupe ◽  
Sergejs Osipovs ◽  
...  
Keyword(s):  
Sex Determination ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Staining Technique ◽  
Life Cycle Stage ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser

AbstractThe roundworms of Trichinella genus are worldwide distributed and their prevalence in nature is high. Trichinella genus parasites are the causative agents of foodborne zoonosis trichinellosis. The main prevention and control of the infection are meat inspection by the magnetic stirrer method for the detection of Trichinella larvae in muscle samples. The treatment can be effective if the parasite is discovered early in the intestinal phase. Once the Trichinella larva has reached the muscle tissue, the parasite remains therein and there is no treatment for this life cycle stage. The Trichinella species is dioecious with separate male and female individuals. The developed staining technique that uses confocal laser scanning microscopy (CLSM) displays sufficient results for Trichinella larvae examination and this protocol is applicable to study the internal and external structures and for the sex determination of T. britovi and T. spiralis larvae samples. In the present study, a luminescent derivative was synthesized and used for staining of T. spiralis and T. britovi larvae samples for the examination by CLSM. Various fixatives, such as AFA, 70% ethanol, and Bouin's and Carnoy's solutions were tested for sample preparation. The synthesized luminescent compound demonstrates best visualization results for samples fixed in Bouin's fixative.

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