scholarly journals Fusarium subglutinans f. sp.pini Represents a Distinct Mating Population in theGibberella fujikuroi Species Complex

1999 ◽  
Vol 65 (3) ◽  
pp. 1198-1201 ◽  
Author(s):  
H. Britz ◽  
T. A. Coutinho ◽  
M. J. Wingfield ◽  
W. F. O. Marasas ◽  
T. R. Gordon ◽  
...  
Keyword(s):  
South Africa ◽  
Species Complex ◽  
Biological Species ◽  
Gibberella Fujikuroi ◽  
Pitch Canker ◽  
Fusarium Subglutinans ◽  
Mating Population ◽  
Standard Tester ◽  
Distinct Taxon

ABSTRACT Fusarium strains in the Gibberella fujikuroi species complex cause diseases on a variety of economically important plants. One of these diseases, pitch canker of Pinus spp., is caused by strains identified asFusarium subglutinans f. sp. pini. Fertile crosses were detected between F. subglutinans f. sp.pini strains from South Africa, California, and Florida.F. subglutinans f. sp. pini strains were not cross-fertile with the standard tester strains of six of the seven other mating populations of G. fujikuroi. Sporadic perithecia with ascospores were obtained in two crosses with the mating population B tester strains. These perithecia were homothallic, and the ascospores derived from these perithecia were vegetatively compatible with the mating population B tester strain parent. We concluded that fertile F. subglutinans f. sp. pini isolates represent a new mating population (mating population H) of G. fujikuroi and that they belong to a unique biological species in a distinct taxon.

scholarly journals Molecular Standardization of Mating Type Terminology in the Gibberella fujikuroi Species Complex

1999 ◽  
Vol 65 (9) ◽  
pp. 4071-4076 ◽  
Author(s):  
Z. Kerényi ◽  
K. Zeller ◽  
L. Hornok ◽  
J. F. Leslie
Keyword(s):  
Dna Sequence ◽  
Mating Type ◽  
Species Complex ◽  
Pcr Primers ◽  
Biological Species ◽  
Gibberella Fujikuroi ◽  
Gibberella Fujikuroi Species Complex ◽  
Sexual Crosses ◽  
Standard Tester ◽  
Sequence Similarities

ABSTRACT Mating type in the Gibberella fujikuroi species complex is controlled by a single locus with two alleles and is usually identified following sexual crosses with standard, female-fertile tester isolates. The mating type alleles have been arbitrarily designated “+” and “−” within each biological species, and the nomenclature is tied to the standard tester strains. We developed a pair of PCR primers that can be used to amplify a unique fragment of one of the mating type alleles (MAT-2) from at least seven of the biological species in this species complex. Based on the amplification pattern, we propose a replacement for the existing, arbitrary +/− terminology that is presently in use. The new terminology is based on DNA sequence similarities between the mating type allele fragments from the biological species of the G. fujikuroi species complex and the corresponding fragments from other filamentous ascomycetes.

scholarly journals Mating populations of Gibberella fujikuroi (Sawada) S. Ito species complex isolating from maize, sorghum and wheat in Serbia

Genetika ◽  
2013 ◽  
Vol 45 (3) ◽  
pp. 749-760 ◽  
Author(s):  
Tamara Kovacevic ◽  
Jelena Levic ◽  
Slavica Stankovic ◽  
Jelena Vukojevic
Keyword(s):  
Sexual Reproduction ◽  
Mating Type ◽  
Species Complex ◽  
World Wide ◽  
Gibberella Fujikuroi ◽  
Fusarium Spp ◽  
Field Isolates ◽  
Mating Population ◽  
The Status ◽  
First Results

The status of fertility and distribution of mating populations in the G. fujikuroi species complex, isolating from maize, sorghum and wheat cultivated under various agroecological conditions of Serbia, have been studied. A total of 79 field isolates of Fusarium spp. in the section Liseola, which had been reciprocally crossed to standard testers (MAT-1 and MAT-2) from each of the four mating populations of the G. fujikuroi species complex, were selected for these studies. Twenty of 79 isolates belonged to the mating population A (G. moniliformis), 22 to the population D (G. intermedia), 17 to the population E (G. subglutinans) and 20 to the population F (G. thapsina). A mating type MAT-1 was dominant in the populations A (14 MATA-1 :6 MATA-2), D (13 MATA-1 :9 MATA-2) and E (10 MATA-1 :7 MATA-2), while MAT-2 prevailed in the population F (6 MATA-1 : 14 MATA-2). The obtained results indicate that the possibility of sexual reproduction of Fusarium spp., belonging to the A, D, E and F mating populations, is not so frequent phenomenon in Serbia as in other regions world-wide. Consequently, these species will be asexually reproduced under field conditions, particularly species belonging to the F population. These are the first results on the characterisation of three (A, E and F) out of four populations of the G. fujikuroi species complex present in Serbia.

Gibberella fujikuroi: available populations and variable traits

1995 ◽  
Vol 73 (S1) ◽  
pp. 282-291 ◽  
Author(s):  
John F. Leslie
Keyword(s):  
Fusarium Moniliforme ◽  
Antifungal Agents ◽  
Geographic Location ◽  
Biological Species ◽  
Fusarium Proliferatum ◽  
Gibberella Fujikuroi ◽  
Hygromycin B ◽  
Complex Species ◽  
General Ability ◽  
Mating Population

Gibberella fujikuroi is a complex species comprised of at least seven distinct mating populations (biological species) with Fusarium anamorphs. These fungi are common pathogens of maize, rice, and sorghum and cause billions of dollars of losses in these crops worldwide. Mating populations are biological species defined by cross-fertility of members of the same mating population with one another, and sterility whenever members of two different mating populations are crossed. Mating populations also differ in their electrophoretic karyotypes, their general ability to synthesize secondary metabolites, and their sensitivity to antifungal agents such as benomyl and hygromycin B. Within a mating population strains can be distinguished on the basis of their ability to form a heterokaryon with one another. This polygenic trait has been used to study the structure of some populations of these fungi. In some cases, pathogenic strains appear to be clones that are limited to a crop and (or) geographic location. More commonly, however, there is significant genetic variability present and sexual reproduction appears to be playing an important role in assorting that variability to different individuals. Key words: Fusarium moniliforme, Fusarium proliferatum, Fusarium subglutinans, population genetics, maize, sorghum.

scholarly journals Gibberella xylarioides Sensu Lato from Coffea canephora: a New Mating Population in the Gibberella fujikuroi Species Complex

2005 ◽  
Vol 71 (12) ◽  
pp. 8466-8471 ◽  
Author(s):  
Pascale C. E. Lepoint ◽  
Françoise T. J. Munaut ◽  
Henri M. M. Maraite
Keyword(s):  
Mating Type ◽  
Species Complex ◽  
Sequence Data ◽  
Coffea Canephora ◽  
Biological Species ◽  
West African ◽  
Gibberella Fujikuroi ◽  
Translation Elongation ◽  
Gibberella Fujikuroi Species Complex

ABSTRACT Gibberella xylarioides Heim & Saccas (presumed anamorph, Fusarium xylarioides Steyaert) is the causal agent of coffee wilt disease, an economically important tracheomycosis in Africa. In vitro crosses carried out with Congolese, Ugandan, and Tanzanian single-ascospore/conidial isolates originating from diseased Coffea canephora/excelsa demonstrated a heterothallic mating system, controlled by a single locus with two alleles, MAT-1 and MAT-2. Compatible isolates produced fertile perithecia within 2 to 8 weeks after mating. Mating type (MAT) was characterized by PCR with primer pairs previously developed for the Gibberella fujikuroi species complex (GFC) and for Fusarium oxysporum. All strains analyzed were morphologically identical and corresponded to Booth's description of the “female” F. xylarioides strain. Based on crossing results and MAT-2/translation elongation 1-α (tef) sequence data, G. xylarioides, as currently understood, is demonstrated to encompass at least three “groups”: G. xylarioides sensu strictu Ia, defined hitherto by two “historical” West African strains originating from the severe 1930s to 1950s epidemic (CBS 25852 and CBS 74979); G. xylarioides sensu strictu Ib, defined by two “historical” Central African lowland strains (DSMZ 62457 and ATCC 15664); and G. xylarioides sensu lato II, containing Congolese, Ugandan, and Tanzanian C. canephora/excelsa isolates. Infertility of crosses between the coffee wilt pathogen and known GFC mating populations demonstrates that G. xylarioides sensu lato constitutes a new biological species within the G. fujikuroi complex. MUCL 44532/MUCL 43887 and MUCL 35223/MUCL 44549 are proposed as G. xylarioides sensu lato II MAT-1/MAT-2 reference mating type tester strains.

scholarly journals Fusarium ananatum sp. nov. in the Gibberella fujikuroi species complex from pineapples with fruit rot in South Africa

2010 ◽  
Vol 114 (7) ◽  
pp. 515-527 ◽  
Author(s):  
Adriaana Jacobs ◽  
Pieter Schalk Van Wyk ◽  
Walter F.O. Marasas ◽  
Brenda D. Wingfield ◽  
Michael J. Wingfield ◽  
...  
Keyword(s):  
South Africa ◽  
Species Complex ◽  
Fruit Rot ◽  
Gibberella Fujikuroi ◽  
Gibberella Fujikuroi Species Complex

scholarly journals PCR-Based Identification of MAT-1 andMAT-2 in the Gibberella fujikuroi Species Complex

2000 ◽  
Vol 66 (10) ◽  
pp. 4378-4382 ◽  
Author(s):  
Emma T. Steenkamp ◽  
Brenda D. Wingfield ◽  
Teresa A. Coutinho ◽  
Kurt A. Zeller ◽  
Michael J. Wingfield ◽  
...  
Keyword(s):  
Species Complex ◽  
Fusarium Verticillioides ◽  
Fusarium Culmorum ◽  
Biological Species ◽  
Gibberella Fujikuroi ◽  
Additional Species ◽  
Individual Mating ◽  
Sexual Cross ◽  
Phylogenetic Lineages ◽  
Gibberella Fujikuroi Species Complex

ABSTRACT All sexually fertile strains in the Gibberella fujikuroi species complex are heterothallic, with individual mating types conferred by the broadly conserved ascomycete idiomorphsMAT-1 and MAT-2. We sequenced both alleles from all eight mating populations, developed a multiplex PCR technique to distinguish these idiomorphs, and tested it with representative strains from all eight biological species and 22 additional species or phylogenetic lineages from this species complex. In most cases, either an ∼800-bp fragment from MAT-2 or an ∼200-bp fragment from MAT-1 is amplified. The amplified fragments cosegregate with mating type, as defined by sexual cross-fertility, in a cross of Fusarium moniliforme (Fusarium verticillioides). Neither of the primer pairs amplify fragments from Fusarium species such as Fusarium graminearum, Fusarium pseudograminearum, andFusarium culmorum, which have, or are expected to have,Gibberella sexual stages but are thought to be relatively distant from the species in the G. fujikuroi species complex. Our results suggest that MAT allele sequences are useful indicators of phylogenetic relatedness in these and otherFusarium species.

scholarly journals Differentiation of Fusarium subglutinansf. sp. pini by Histone Gene Sequence Data

1999 ◽  
Vol 65 (8) ◽  
pp. 3401-3406 ◽  
Author(s):  
E. T. Steenkamp ◽  
B. D. Wingfield ◽  
T. A. Coutinho ◽  
M. J. Wingfield ◽  
W. F. O. Marasas
Keyword(s):  
Species Complex ◽  
Gene Sequence ◽  
Fragment Length Polymorphism ◽  
Sequence Data ◽  
Histone H3 ◽  
Biological Species ◽  
Gibberella Fujikuroi ◽  
Exon Sequence ◽  
Pathogenicity Tests ◽  
Histone H3 Gene

ABSTRACT Fusarium subglutinans f. sp. pini (=F. circinatum) is a pathogen of pine and is one of eight mating populations (i.e., biological species) in the Gibberella fujikuroi species complex. This species complex includes F. thapsinum, F. moniliforme (= F. verticillioides), F. nygamai, and F. proliferatum, as well as F. subglutinans associated with sugarcane, maize, mango, and pineapple. Differentiating these forms of F. subglutinans usually requires pathogenicity tests, which are often time-consuming and inconclusive. Our objective was to develop a technique to differentiate isolates of F. subglutinans f. sp. pini from other isolates identified as F. subglutinans. We sequenced the histone H3 gene from a representative set of Fusarium isolates. The H3 gene sequence was conserved and contained two introns in all the isolates studied. From both the intron and the exon sequence data, we developed a PCR-restriction fragment length polymorphism technique that reliably distinguishes F. subglutinans f. sp.pini from the other biological species in the G. fujikuroi species complex.

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