A Randomly Amplified Polymorphic DNA Marker Specific for the Bacillus cereus Group Is Diagnostic forBacillus anthracis
1999 ◽
Vol 65
(3)
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pp. 1298-1303
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ABSTRACT Aiming to develop a DNA marker specific for Bacillus anthracis and able to discriminate this species fromBacillus cereus, Bacillus thuringiensis, andBacillus mycoides, we applied the randomly amplified polymorphic DNA (RAPD) fingerprinting technique to a collection of 101 strains of the genus Bacillus, including 61 strains of theB. cereus group. An 838-bp RAPD marker (SG-850) specific for B. cereus, B. thuringiensis, B. anthracis, and B. mycoides was identified. This fragment included a putative (366-nucleotide) open reading frame highly homologous to the ypuA gene of Bacillus subtilis. The restriction analysis of the SG-850 fragment withAluI distinguished B. anthracis from the other species of the B. cereus group.
2006 ◽
Vol 349
(4)
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pp. 1214-1219
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1988 ◽
Vol 8
(9)
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pp. 3898-3905
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2003 ◽
Vol 69
(8)
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pp. 4502-4510
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1988 ◽
Vol 8
(7)
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pp. 2797-2803
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2009 ◽
Vol 23
(6)
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pp. 291-297
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Keyword(s):
2003 ◽
Vol 69
(6)
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pp. 3658-3662
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Keyword(s):
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