STUDI PERAKITAN KELAPA HIBRIDA GSK x DMT BERDASARKAN PENANDA RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA)

EUGENIA ◽  
2008 ◽  
Vol 14 (1) ◽  
Author(s):  
Semuel D. Runtunuwu ◽  
Hengky Novarianto ◽  
Heldering Tampake ◽  
Edy F. Lengkong
Keyword(s):  
Dna Marker ◽  
Rapd Marker ◽  
High Yield ◽  
Randomly Amplified Polymorphic Dna ◽  
Germination Time ◽  
Flower Production ◽  
Short Time

ABSTRACT   Runtunuwu, S.D. et al. 2008. Assembling Hybrid Coconut of GSK x DMT Based on RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA) Marker. Eugenia 14 (1) : 134-152.   The aimed of this research was : 1. assembling hybrid coconut GSK x DMT (Genjah Salak x Dalam Mapanget) that seeds growth was relatifly homogeneous based on RAPD (Randomly Amplified Polymorphic DNA) marker and 2. to found the assembling method of hybrid coconut that will produce massive seeds relatifely short time will homogeneous plant. It was 65 individu trees observe for the average of famale flower per bunch. The result was 25 individu of coconut GSK has the average flower production > 40 per bunch was analyze the homogeneous genetic with the RAPD marker. Based on the analyze RAPD that were 25 individu of GSK coconut trees have the same genetic average 88 % and 14 individu among that was 100 % have same genetic. Further more that 14 individu of GSK was crossing with the 3 individu of DMT that have high yield per year its was DMT 1188, 1172 and 781. Based on the evaluation for the color of buds, high of buds, the steam circle, the petiole color and the germination time of hybrid coconut seeds from the crossing of GSK x DMT 1188 produce more than    70 % seeds that have same genetic, also for crossing of GSK x DMT 1172 have 9 combination and have more than 70 % that same genetic, 10 combination from crossing GSK x DMT 781 have more than 80 % same seeds growth. Therefore, using the RAPD marker were successfully produced 28 crossing of the hybrid coconut GSK x DMT that have relatifly homogeneous seeds growth.   Keywords : assembling, hybrid coconut GSK x DMT, RAPD.

scholarly journals A Randomly Amplified Polymorphic DNA Marker Specific for the Bacillus cereus Group Is Diagnostic forBacillus anthracis

1999 ◽  
Vol 65 (3) ◽  
pp. 1298-1303 ◽  
Author(s):  
Daniele Daffonchio ◽  
Sara Borin ◽  
Giuseppe Frova ◽  
Romina Gallo ◽  
Elena Mori ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Dna Marker ◽  
Restriction Analysis ◽  
Rapd Marker ◽  
Open Reading Frame ◽  
The Other ◽  
Randomly Amplified Polymorphic Dna ◽  
Bacillus Mycoides ◽  
Bacillus Cereus Group ◽  
Reading Frame

ABSTRACT Aiming to develop a DNA marker specific for Bacillus anthracis and able to discriminate this species fromBacillus cereus, Bacillus thuringiensis, andBacillus mycoides, we applied the randomly amplified polymorphic DNA (RAPD) fingerprinting technique to a collection of 101 strains of the genus Bacillus, including 61 strains of theB. cereus group. An 838-bp RAPD marker (SG-850) specific for B. cereus, B. thuringiensis, B. anthracis, and B. mycoides was identified. This fragment included a putative (366-nucleotide) open reading frame highly homologous to the ypuA gene of Bacillus subtilis. The restriction analysis of the SG-850 fragment withAluI distinguished B. anthracis from the other species of the B. cereus group.

Assessment of genome origins and genetic diversity in the genus Eleusine with DNA markers

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 757-763 ◽  
Author(s):  
Shanmukhaswami S. Salimath ◽  
Antonio C. de Oliveira ◽  
Jeffrey L. Bennetzen ◽  
Ian D. Godwin
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Finger Millet ◽  
Dna Marker ◽  
Fragment Length Polymorphism ◽  
Eleusine Coracana ◽  
Randomly Amplified Polymorphic Dna ◽  
Simple Sequence ◽  
Restriction Fragment Length

Finger millet (Eleusine coracana), an allotetraploid cereal, is widely cultivated in the arid and semiarid regions of the world. Three DNA marker techniques, restriction fragment length polymorphism (RFLP), randomly amplified polymorphic DNA (RAPD), and inter simple sequence repeat amplification (ISSR), were employed to analyze 22 accessions belonging to 5 species of Eleusine. An 8 probe – 3 enzyme RFLP combination, 18 RAPD primers, and 6 ISSR primers, respectively, revealed 14, 10, and 26% polymorphism in 17 accessions of E. coracana from Africa and Asia. These results indicated a very low level of DNA sequence variability in the finger millets but did allow each line to be distinguished. The different Eleusine species could be easily identified by DNA marker technology and the 16% intraspecific polymorphism exhibited by the two analyzed accessions of E. floccifolia suggested a much higher level of diversity in this species than in E. coracana. Between species, E. coracana and E. indica shared the most markers, while E. indica and E. tristachya shared a considerable number of markers, indicating that these three species form a close genetic assemblage within the Eleusine. Eleusine floccifolia and E. compressa were found to be the most divergent among the species examined. Comparison of RFLP, RAPD, and ISSR technologies, in terms of the quantity and quality of data output, indicated that ISSRs are particularly promising for the analysis of plant genome diversity.Key words: Eleusine coracana, finger millet, genome analysis, microsatellites, randomly amplified polymorphic DNA, restriction fragment length polymorphism, simple sequence repeats.

Randomly amplified polymorphic DNA linkage relationships in different Norway spruce populations

2001 ◽  
Vol 31 (8) ◽  
pp. 1456-1461
Author(s):  
M Troggio ◽  
T L Kubisiak ◽  
G Bucci ◽  
P Menozzi
Keyword(s):  
Rapd Markers ◽  
Rapd Marker ◽  
Population Based ◽  
Italian Population ◽  
Randomly Amplified Polymorphic Dna ◽  
Linkage Groups ◽  
Consensus Map ◽  
Norwegian Population ◽  
Marker Loci ◽  
Linkage Relationships

We tested the constancy of linkage relationships of randomly amplified polymorphic DNA (RAPD) marker loci used to construct a population-based consensus map in material from an Italian stand of Picea abies (L.) Karst. in 29 individuals from three Norwegian populations. Thirteen marker loci linked in the Italian stand did show a consistent locus ordering in the Norwegian population. The remaining 16 unlinked marker loci were spread over different linkage groups and (or) too far apart both in the population map and in this study. The limited validity of RAPD markers as genomic "hallmarks" resilient across populations is discussed. We also investigated the reliability of RAPD markers; only 58% of the RAPD markers previously used to construct the consensus map in the Italian population were repeatable in the same material. Of the repeatable ones 76.3% were amplified and found polymorphic in 29 megagametophyte sibships from three Norwegian populations.

Microwave synthesis, photophysical properties of novel fluorescent iminocoumarins and their application in textile printing

2015 ◽  
Vol 44 (2) ◽  
pp. 87-93 ◽  
Author(s):  
G.H. Elgemeie ◽  
K.A. Ahmed ◽  
E.A. ahmed ◽  
M.H. helal ◽  
D.M. Masoud
Keyword(s):  
Blue Light ◽  
Microwave Synthesis ◽  
Photophysical Properties ◽  
Ethyl Cyanoacetate ◽  
High Yield ◽  
The Novel ◽  
Dioxane Solution ◽  
Content Type ◽  
Textile Printing ◽  
Short Time

Purpose – This paper aims to synthesise coumarine flourescent dyes from a cheap material in a very short time with a very high yield, and by using a clean green chemistry. Design/methodology/approach – Efficient microwave synthesis for some novel iminocoumarins starts from the reaction of p-phenyl-enediamine and ethyl cyanoacetate followed by cyclocondensation with salicylaldehyde derivatives. Findings – The synthesized iminocoumarine compounds were characterized by spectroscopic methods. Absorption and fluorescence spectra of the compounds were also recorded. All compounds were fluorescent in 1,4-dioxane solution, they all emitted blue light (440-460 nm). The printing properties were studied, and their applications on printing polyester and polyamide fabrics were studied by silk screen printing. Originality/value – The authors designed efficient microwavel synthesis for some novel iminocoumarine derivatives; The novel procedure features short-reaction time, moderate yields and simple workup; All compounds were fluorescent in 1,4-dioxane solution, and they all emitted blue light; The authors studied their application in printing polyester and polyamide fabrics.

Studies on 203Hg-Chlormerodrin Rapid Synthesis and Measurement of 203Hg-Chlormerodrin and Determination of its Fate in Rats

1970 ◽  
Vol 09 (03) ◽  
pp. 259-267
Author(s):  
Akira Hasegawa ◽  
Goro Urakubo ◽  
Akira Tanaka
Keyword(s):  
Isotopic Exchange ◽  
Practical Method ◽  
Renal Tissue ◽  
Whole Body ◽  
High Yield ◽  
Rapid Synthesis ◽  
Isotopic Exchange Reaction ◽  
Preferential Binding ◽  
Short Time

SummaryThe rapid synthesis of 203Hg-chlormerodrin by an isotopic exchange reaction in organic solvents was investigated using a mixture of inactive chlormerodrin and 203Hg-mercuric chloride. This reaction is promising as a practical method since 203Hg-CM can be so prepared in a short time. In methanol, 3-acetoxy-mercuri-2 methoxypropylurea exchanged at room temperature with 203Hg-mercuric acetate to a relatively high yield. The most suitable solvent for paper chromatography of CM was found to be a mixture of dimethylformamide, water and methanol (5 : 1 : 14 v/v). The fate and distribution of 203Hg-CM in rats were studied after a single oral dose and the maximum of activity was observed in the kidneys during the first 6 hours. A preferential binding of Hg by renal tissue was found. The biological half-life in the whole body was about 18.5 days. This compound was excreted mainly via the faeces but small amounts were excreted in the urine.

scholarly journals Strain-Specific Identification of ProbioticLactobacillus rhamnosus with Randomly Amplified Polymorphic DNA-Derived PCR Primers

1998 ◽  
Vol 64 (12) ◽  
pp. 4816-4819 ◽  
Author(s):  
Anu Tilsala-Timisjärvi ◽  
Tapani Alatossava
Keyword(s):  
Rapd Marker ◽  
Lactobacillus Rhamnosus ◽  
Pcr Primers ◽  
Randomly Amplified Polymorphic Dna ◽  
Significant Similarity ◽  
Specific Pcr ◽  
Protein Encoding ◽  
Sequence Elements ◽  
Bacterium Species ◽  
Insertion Sequence Elements

ABSTRACT In the present work, strain-specific PCR primers forLactobacillus rhamnosus Lc 1/3 are described. The randomly amplified polymorphic DNA (RAPD) technique was used to produce potential strain-specific markers. They were screened for specificity by hybridization with DNA from 11 L. rhamnosus strains. A 613-bp RAPD marker found to be strain-specific was sequenced, and a primer pair specific to L. rhamnosus Lc 1/3 was constructed based on the sequence. The primer pair was tested with 11Lactobacillus species and 11 L. rhamnosusstrains and was found to be strain specific. The nucleotide sequence of the specific RAPD marker was found to contain part of a protein encoding region which showed significant similarity to several transposases for insertion sequence elements of various bacteria, including other lactic acid bacterium species.

scholarly journals Synthesis of 1-(β-coumarinyl)-1-(β-indolyl)trifluoroethanols through regioselective Friedel–Crafts alkylation of indoles with β-(trifluoroacetyl)coumarins catalyzed by Sc(OTf)3

RSC Advances ◽  
2020 ◽  
Vol 10 (24) ◽  
pp. 13929-13935 ◽  
Author(s):  
Lijun Shi ◽  
Ying Liu ◽  
Caixia Wang ◽  
Xinxin Yuan ◽  
Xiaobiao Liu ◽  
...  
Keyword(s):  
High Yield ◽  
Short Time

A Friedel–Crafts alkylation of indoles with β-(trifluoroacetyl)coumarins catalyzed by Sc(OTf)3 to afford 1-(β-coumarinyl)-1-(β-indolyl)trifluoroethanols in a short time and high yield was developed.

scholarly journals Post-drying of energy sorrel in a grate stock

2012 ◽  
Vol 50 (No. 1) ◽  
pp. 15-22
Author(s):  
P. Hutla ◽  
J. Mazancová
Keyword(s):  
Water Content ◽  
Electric Energy ◽  
Energy Crop ◽  
Yield Potential ◽  
High Yield ◽  
Large Capacity ◽  
The Czech Republic ◽  
Time Switching ◽  
Operational Conditions ◽  
Short Time

Energy sorrel is a crop with high-yield potential and belongs among the most promissing energy crop for the Czech Republic. The suitable processing technology is harvest by the harvesting cutter with subsequent short-time storage and post-drying of chopped material in the large-capacity hayloft. For chopped sorrel were found-out hydraulic air losses during its passing through the stored layer and they were compared with values for stored forage. Two methods of drying ventilators controlling in the large-capacity heyloft were compared with the regime of time switching within chopped sorrel drying. Electric energy comsumption for ventilators drive in different regimes depends on water content in the material. Method of ventilators or time switching controlling has no effect on drying process result, thus even on water content reduction in the dried material. Under operational conditions the possibility of the chopped energy sorrel in large-capacity heyloft was verified.

scholarly journals Valorisation of Chinese hickory shell as novel sources for the efficient production of xylooligosaccharides

2021 ◽  
Author(s):  
Zhi-Kun Wang ◽  
Caoxing Huang ◽  
Jun-Lei Zhong ◽  
Lv Tang ◽  
Bing Li ◽  
...  
Keyword(s):  
Food Waste ◽  
Liquid Fraction ◽  
Hydrothermal Process ◽  
Maximum Yield ◽  
High Yield ◽  
Efficient Production ◽  
Pretreatment Condition ◽  
Sustainable Technologies ◽  
Chemical Structures ◽  
Short Time

Abstract Chinese hickory shell, a by-product of the food industry, is still not utilized and urgent to develop sustainable technologies for its valorization. This research focuses on the systematical evaluation of degraded products and xylooligosaccharide production with high yield from the shell via hydrothermal process. The results indicated that the pretreatment condition strongly affected the chemical structures and compositions of the liquid fraction. The maximum yield of XOS (55.3 wt%) with limitation of by-products formation was achieved at 160 oC for 2 h. High temperature (220 oC) and short time (0.5 h) contributed to hydrolysis of xylooligosaccharide with high DP to yield 37.5 wt% xylooligosaccharide with DP from 2 to 6. xylooligosaccharide obtained mainly consisted of xylan with branches according to the HSQC NMR analysis. Overall, the production of XOS with a high yield from food waste will facilitate the valorization of food waste in the biorefinery industry.

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