scholarly journals Natural Communities of Novel Archaea and Bacteria with a String-of-Pearls-Like Morphology: Molecular Analysis of the Bacterial Partners

2002 ◽  
Vol 68 (2) ◽  
pp. 933-937 ◽  
Author(s):  
Christine Moissl ◽  
Christian Rudolph ◽  
Robert Huber
Keyword(s):  
Phylogenetic Analysis ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Major Constituent ◽  
Filamentous Bacteria ◽  
In Series ◽  
Taxonomic Affiliation ◽  
String Of Pearls ◽  
Natural Communities

ABSTRACT A recently discovered bacterial/archaeal association, growing in a string-of-pearls-like structure, thrives in the cold (∼10�C) sulfidic marsh water of the Sippenauer Moor near Regensburg, Bavaria, Germany. It forms characteristic, macroscopically visible globules, the pearls, containing microcolonies of novel euryarchaeota, which are surrounded by mainly filamentous bacteria (C. Rudolph, G. Wanner, and R. Huber, Appl. Environ. Microbiol. 67:2336-2344, 2001). Single pearls in series are connected by white threads. Here we report the first detailed molecular investigations of the taxonomic affiliation of the bacteria contributing to the strings of pearls. Phylogenetic analysis showed the dominance of a single phylotype (clone sipK4) within single pearls most closely related to Thiothrix unzii. The presence of Thiothrix sipK4 as a major constituent of single pearls and of the pearl-connecting white threads was verified by fluorescence in situ hybridization.

scholarly journals Phylogenetic analysis of multiprobe fluorescence in situ hybridization data from tumor cell populations

2013 ◽  
Vol 29 (13) ◽  
pp. i189-i198 ◽  
Author(s):  
Salim Akhter Chowdhury ◽  
Stanley E. Shackney ◽  
Kerstin Heselmeyer-Haddad ◽  
Thomas Ried ◽  
Alejandro A. Schäffer ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
In Situ Hybridization ◽  
Tumor Cell ◽  
Fluorescence In Situ Hybridization ◽  
Cell Populations ◽  
Hybridization Data

scholarly journals Use of Combined Microautoradiography and Fluorescence In Situ Hybridization To Determine Carbon Metabolism in Mixed Natural Communities of Uncultured Bacteria from the GenusAchromatium

2000 ◽  
Vol 66 (10) ◽  
pp. 4518-4522 ◽  
Author(s):  
N. D. Gray ◽  
R. Howarth ◽  
R. W. Pickup ◽  
J. Gwyn Jones ◽  
I. M. Head
Keyword(s):  
In Situ Hybridization ◽  
Organic Carbon ◽  
Fluorescence In Situ Hybridization ◽  
Carbon Metabolism ◽  
Carbon Sources ◽  
Uncultured Bacteria ◽  
Sulfur Bacteria ◽  
Organic Carbon Sources ◽  
Natural Communities

ABSTRACT Combined microautoradiography and fluorescence in situ hybridization (FISH) was used to investigate carbon metabolism in uncultured bacteria from the genus Achromatium. All of theAchromatium species identified in a freshwater sediment from Rydal Water, Cumbria, United Kingdom, which were distinguishable only by FISH, assimilated both [14C]bicarbonate and [14C]acetate. This extends previous findings thatAchromatium spp. present at another location could only utilize organic carbon sources. Achromatium spp., therefore, probably exhibit a range of physiologies, i.e., facultative chemolithoautotrophy, mixotrophy, and chemoorganoheterotrophy, similar to other large sulfur bacteria (e.g., Beggiatoa spp.).

scholarly journals α-Proteobacterial Symbionts of Marine Bryozoans in the Genus Watersipora

2006 ◽  
Vol 73 (1) ◽  
pp. 303-311 ◽  
Author(s):  
Christine M. Anderson ◽  
Margo G. Haygood
Keyword(s):  
Phylogenetic Analysis ◽  
In Situ Hybridization ◽  
16S Rrna ◽  
Fluorescence In Situ Hybridization ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Bacterial Symbionts ◽  
California Coast ◽  
Specific Fluorescence

ABSTRACT Bacterial symbionts that resembled mollicutes were discovered in the marine bryozoan Watersipora arcuata in the 1980s. In this study, we used PCR and sequencing of 16S rRNA genes, specific fluorescence in situ hybridization, and phylogenetic analysis to determine that the bacterial symbionts of “W. subtorquata” and “W. arcuata” from several locations along the California coast are actually closely related α-Proteobacteria, not mollicutes. We propose the names “Candidatus Endowatersipora palomitas” and “Candidatus Endowatersipora rubus” for the symbionts of “W. subtorquata” and “W. arcuata,” respectively.

890: Multiple Fluorescence in Situ Hybridization (FISH) Probes Increase Sensitivity for Detection of Bladder Cancer

2006 ◽  
Vol 175 (4S) ◽  
pp. 287-288 ◽  
Author(s):  
Juliann M. Dziubinski ◽  
Michael F. Sarosdy ◽  
Paul R. Kahn ◽  
Mark D. Ziffer ◽  
William R. Love ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization

588: Detection of Chromosome 8 Alterations in Paired Needle Biopsy and Prostatectomy Specimens Using Fluorescence In-Situ Hybridization

2004 ◽  
Vol 171 (4S) ◽  
pp. 156-156
Author(s):  
Chandler D. Dora ◽  
Yasushi Kondo ◽  
Fusheng X. Lan ◽  
Jeffrey M. Slezak ◽  
Erik J. Bergstralh ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Needle Biopsy ◽  
Chromosome 8
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