scholarly journals Large-Scale Purification of Ribonucleic Acid Tumor Viruses by Use of Continuous-Flow Density Gradient Centrifugation

1973 ◽  
Vol 26 (3) ◽  
pp. 452-454
Author(s):  
D. P. Grandgenett ◽  
K. Brackmann ◽  
M. Green
Keyword(s):  
Density Gradient ◽  
Ribonucleic Acid ◽  
Continuous Flow ◽  
Large Scale ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Flow Density ◽  
Tumor Viruses

The large-scale preparation of purified sporozoites of Eimeria spp. by metrizamide density-gradient centrifugation

Parasitology ◽  
1984 ◽  
Vol 88 (3) ◽  
pp. 515-519 ◽  
Author(s):  
M. H. Wisher ◽  
M. E. Rose
Keyword(s):  
Density Gradient ◽  
Large Scale ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Density Gradients ◽  
Large Scale Preparation ◽  
Eimeria Spp ◽  
Scale Preparation ◽  
The Mean

SUMMARYA method is described for the reproducible purification of greater than 1·0 × 108 sporozoites of Eimeria spp. by centrifugation on metrizamide density-gradients. The mean contamination of the purified sporozoite fraction by sporocysts and sporocyst debris was less than 6% and the recovered sporozoites were fully viable, infective and ultrastructurally intact.

scholarly journals Nucleic acid enzymology of extremely halophilic bacteria. Gel-filtration and density-gradient-centrifugation studies of the molecular weights of Halobacterium cutirubrum polynucleotide phosphorylase and deoxyribonucleic acid- and ribonucleic acid-dependent ribonucleic acid polymerases

1971 ◽  
Vol 121 (4) ◽  
pp. 635-641 ◽  
Author(s):  
B. Gregory Louis ◽  
Pearl I. Peterkin ◽  
P. S. Fitt
Keyword(s):  
Rna Polymerase ◽  
Density Gradient ◽  
Ribonucleic Acid ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Halophilic Bacteria ◽  
Gel Filtration ◽  
Polynucleotide Phosphorylase ◽  
Sucrose Density Gradient Centrifugation ◽  
Molecular Weights

1. Conditions have been established for the estimation of molecular weights of proteins by analytical gel filtration and sucrose-density-gradient centrifugation in 2.5m-potassium chloride–1m-sodium chloride; Halobacterium cutirubrum polynucleotide phosphorylase, DNA-dependent RNA polymerase and RNA-dependent RNA polymerase have been studied by these methods. 2. The RNA-dependent polymerase has also been studied by density-gradient centrifugation in the absence of salt. 3. All three proteins are of unusually low molecular weight compared with similar enzymes from non-halophilic bacteria.

Sign in / Sign up

Export Citation Format

Share Document