scholarly journals A Photostable Green Fluorescent Protein Variant for Analysis of Protein Localization in Candida albicans

2009 ◽  
Vol 9 (1) ◽  
pp. 224-226 ◽  
Author(s):  
Chengda Zhang ◽  
James B. Konopka
Keyword(s):  
Candida Albicans ◽  
Green Fluorescent Protein ◽  
Codon Usage ◽  
Signal Intensity ◽  
Fluorescent Protein ◽  
Protein Localization ◽  
Protein Variant ◽  
Green Fluorescent ◽  
Green Fluorescent Protein Variant

ABSTRACT Fusions to the green fluorescent protein (GFP) are an effective way to monitor protein localization. However, altered codon usage in Candida species has delayed implementation of new variants. Examination of three new GFP variants in Candida albicans showed that one has higher signal intensity and increased resistance to photobleaching.

scholarly journals Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D. 1. Mutagenesis and Structural Studies†,‡

Biochemistry ◽  
2007 ◽  
Vol 46 (43) ◽  
pp. 12005-12013 ◽  
Author(s):  
Xiaokun Shu ◽  
Karen Kallio ◽  
Xinghua Shi ◽  
Paul Abbyad ◽  
Pakorn Kanchanawong ◽  
...  
Keyword(s):  
Excited State ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Structural Studies ◽  
Protein Variant ◽  
Excited State Dynamics ◽  
Green Fluorescent ◽  
Green Fluorescent Protein Variant

Slow Exchange in the Chromophore of a Green Fluorescent Protein Variant

2002 ◽  
Vol 124 (27) ◽  
pp. 7932-7942 ◽  
Author(s):  
Markus H. J. Seifert ◽  
Dorota Ksiazek ◽  
M. Kamran Azim ◽  
Pawel Smialowski ◽  
Nediljko Budisa ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Protein Variant ◽  
Slow Exchange ◽  
Green Fluorescent ◽  
Green Fluorescent Protein Variant

Photoreversible Dark State in a Tristable Green Fluorescent Protein Variant

2003 ◽  
Vol 107 (7) ◽  
pp. 1679-1684 ◽  
Author(s):  
Riccardo Nifosì ◽  
Aldo Ferrari ◽  
Caterina Arcangeli ◽  
Valentina Tozzini ◽  
Vittorio Pellegrini ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Protein Variant ◽  
Dark State ◽  
Green Fluorescent ◽  
Green Fluorescent Protein Variant

scholarly journals An alternative excited-state proton transfer pathway in green fluorescent protein variant S205V

2007 ◽  
Vol 16 (12) ◽  
pp. 2703-2710 ◽  
Author(s):  
Xiaokun Shu ◽  
Pavel Leiderman ◽  
Rinat Gepshtein ◽  
Nicholas R. Smith ◽  
Karen Kallio ◽  
...  
Keyword(s):  
Excited State ◽  
Green Fluorescent Protein ◽  
Proton Transfer ◽  
Fluorescent Protein ◽  
Protein Variant ◽  
Excited State Proton Transfer ◽  
Green Fluorescent ◽  
Green Fluorescent Protein Variant

scholarly journals Green Fluorescent Protein as a Marker inRickettsia typhi Transformation

1999 ◽  
Vol 67 (7) ◽  
pp. 3308-3311 ◽  
Author(s):  
Jill Michelle Troyer ◽  
Suzana Radulovic ◽  
Abdu F. Azad
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Fragment Length Polymorphism ◽  
Vero Cells ◽  
Intracellular Pathogen ◽  
Protein Variant ◽  
Cell Sorter ◽  
Fusion Construct ◽  
Green Fluorescent ◽  
Green Fluorescent Protein Variant

ABSTRACT Transformation of rickettsiae is a recent accomplishment, but utility of this technique is limited due to the paucity of selectable markers suitable for use in this intracellular pathogen. We chose a green fluorescent protein variant optimized for fluorescence under UV lights (GFPUV) as a fluorometric marker and transformed Rickettsia typhi with anrpoB-GFPUV fusion construct. The rickettsiae were subsequently grown in Vero cells, and cultures were screened by PCR and restriction fragment length polymorphism (RFLP) to confirm incorporation of the rpoB-GFPUV construct. Cultures were then analyzed by flow cytometry for detection of GFPUV expression, and transformed R. typhi were isolated in a fluorescence-activated cell sorter. This is the first report of transformation of rickettsiae with a nonrickettsial (GFPUV) gene.

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