scholarly journals Adsorption of Lysozyme from Human Whole Saliva by Streptococcus sanguis 903 and Other Oral Microorganisms

1982 ◽  
Vol 36 (1) ◽  
pp. 148-159 ◽  
Author(s):  
Nancy J. Laible ◽  
Greg R. Germaine
Keyword(s):  
Streptococcus Sanguis ◽  
Whole Saliva ◽  
Oral Microorganisms

Changes of Serological Activity by α-L-Fucosidase Isolated from Streptococcus sanguis ATCC 10557

1979 ◽  
Vol 58 (2) ◽  
pp. 656-659 ◽  
Author(s):  
S. Shizukuishi ◽  
T. Taniguchi ◽  
S. Shibata ◽  
R. Nakamura ◽  
A. Tsunemitsu ◽  
...  
Keyword(s):  
Blood Group ◽  
Ovarian Cyst ◽  
Cyst Fluid ◽  
Concomitant Increase ◽  
Streptococcus Sanguis ◽  
Whole Saliva ◽  
Blood Group Substances ◽  
Serological Activity ◽  
Ovarian Cyst Fluid

a-L-Fucosidase isolated from the growth culture of Streptococcus sanguis ATCC 10557 acted on H- and Leb-blood group substances in porcine gastric lining, human gastric lining, human ovarian cyst fluid and human whole saliva, with consequent loss of H- and Leb -activities and a concomitant increase of Lea activity.

scholarly journals Lymphoproliferative responses to oral bacteria in humans with varying severities of periodontal disease

1980 ◽  
Vol 28 (3) ◽  
pp. 777-784
Author(s):  
M R Patters ◽  
P Chen ◽  
J McKenna ◽  
R J Genco
Keyword(s):  
Periodontal Disease ◽  
Peripheral Blood Lymphocytes ◽  
Oral Bacteria ◽  
Streptococcus Sanguis ◽  
Disease States ◽  
Lymphocyte Blastogenesis ◽  
Actinomyces Species ◽  
Subgingival Microflora ◽  
Oral Microorganisms

We assessed in vitro the lymphocyte blastogenic resp onsess of peripheral blood lymphocytes to antigen extracts of a large battery of oral microorganisms in a population of humans with varying severities of periodontal disease. When the magnitudes and frequencies of statistically positive blastogenic responses to various oral microorganisms were compared, three general patterns emerged. The Actinomyces species proved to be potent stimulators of lymphocyte blastogenesis in most subjects tested, whereas Streptococcus sanguis, Campylobacter, and Eikenella corrodens stimulated few individuals. The response to these organisms correlated poorly with the severity of periodontal disease in the tested patients. However, several gram-negative anaerobic organisms, including Bacteroides asaccharolyticus and Treponema denticola, elicited statistically more frequent positive response in subjects with destructive periodontitis compared with patients with gingivitis. These results, taken together with recent microbiological findings, suggest that the specificity of the lymphocyte blastogenic response to antigens of oral bacteria correlates with the presence of these organisms in the subgingival microflora during various periodontal disease states.

scholarly journals Blood contamination in salivary diagnostics: current methods and their limitations

2019 ◽  
Vol 57 (8) ◽  
pp. 1115-1124 ◽  
Author(s):  
Jeong-Hyun Kang ◽  
Hong-Seop Kho
Keyword(s):  
Plasma Proteins ◽  
Visual Inspection ◽  
Web Of Science ◽  
Salivary Flow ◽  
Diagnostic Value ◽  
Gonadal Hormones ◽  
Blood Contamination ◽  
Whole Saliva ◽  
Salivary Diagnostics ◽  
Oral Microorganisms

Abstract The use of saliva samples in clinical studies has increased. However, the diagnostic value of whole saliva is compromised in the presence of blood contamination, owing to the higher levels of analytes in blood compared with those in saliva. The aim of this study was to review the existing methods and their limitations for measuring the levels of blood contamination in saliva. A literature search was performed using Web of Science, SCOPUS, and PubMed databases and 49 articles dealing with salivary diagnostics and measurements of blood contamination were included. Five methods for measuring the degree of blood components in saliva were discussed, including “visual inspection”, use of “strip for urinalysis”, and detection of plasma proteins such as “hemoglobin”, “albumin”, and “transferrin”. Each method has its limitations, and transferrin has been regarded as the most reliable and valid marker for blood contamination in saliva. However, transferrin in whole saliva may not be solely a product of blood, and its level in whole saliva can be influenced by several factors such as age, gonadal hormones, salivary flow rate, chewing performance, and oral microorganisms. In conclusion, when quantitatively analyzing whole saliva samples, the influence of blood contamination should be considered.

Release of cariostatic agents from a new buffering fluoride- and xylitol-containing lozenge to human whole saliva in vivo

1997 ◽  
Vol 24 (5) ◽  
pp. 325-331 ◽  
Author(s):  
J. TENOVUO ◽  
T. HURME ◽  
A. AHOLA ◽  
C. SVEDBERG ◽  
I. OSTELA ◽  
...  
Keyword(s):  
Whole Saliva

Responses of Platelets to Strains of Streptococcus sanguis: Findings in Healthy Subjects, Bernard-Soulier, Glanzmann’s, and Collagen-Unresponsive Patients

1987 ◽  
Vol 57 (02) ◽  
pp. 222-225 ◽  
Author(s):  
A H Soberay ◽  
M C Herzberg ◽  
J D Rudney ◽  
H K Nieuwenhuis ◽  
J J Sixma ◽  
...  
Keyword(s):  
Platelet Aggregation ◽  
Healthy Subjects ◽  
Normal Subjects ◽  
Membrane Glycoprotein ◽  
Response Mechanism ◽  
Platelet Response ◽  
Induce Platelet Aggregation ◽  
Streptococcus Sanguis ◽  
Lag Times ◽  
Bernard Soulier Syndrome

SummaryThe ability of endocarditis and dental strains of Streptococcus sanguis to induce platelet aggregation in plasma (PRP) from normal subjects were examined and compared to responses of PRP with known platelet membrane glycoprotein (GP) and response defects. S. sanguis strains differed in their ability to induce normal PRPs to aggregate. Strains that induced PRP aggregation in more than 60% of donors were significantly faster agonists (mean lag times to onset of aggregation less than 6 min) than those strains inducing response in PRPs of fewer than 60% of donors.Platelets from patients with Bernard-Soulier syndrome aggregated in response to strains of S. sanguis. In contrast, platelets from patients with Glanzmann’s thrombasthenia and from a patient with a specific defect in response to collagen were unresponsive to S. sanguis. These observations show that GPIb and V are not essential, but GPIIb-IIIa and GPIa are important in the platelet response mechanism to S. sanguis. Indeed, the data suggests that the platelet interaction mechanisms of S. sanguis and collagen may be similar.

An In Vitro Analysis of Sodium Hypochlorite Decontamination for the Reuse of Implant Healing Abutments

2020 ◽  
Author(s):  
Ahmad Almehmadi
Keyword(s):  
Sodium Hypochlorite ◽  
Bacterial Culture ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Vitro Study ◽  
Sem Images ◽  
Streptococcus Sanguis ◽  
Implant Dentistry ◽  
Vitro Analysis

Abstract The re-use of healing abutments (HAs) has become common practice in implant dentistry for economic concerns and the aim of this in-vitro study was to assess the effect of sodium hypochlorite (NaOCl) in decontamination of HAs. 122 HAs (Used and sterilized n=107; New n=15) were procured from 3 centers, of which 3 samples were discarded due to perforation in sterilization pouch.  For sterility assessment, the used HAs (n=80) were cultured in Brain Heart Infusion Broth (BHI) and Potato Dextrose Agar (PDA), bacterial isolates were identified in 7 samples. Also, 24 used HAs were stained with Phloxine B, photographed and compared to new HAs (n=5). Scanning electron microscope (SEM) assessed the differences between the two sets of HAs, following which the 7 contaminated HAs along with 24 used HAs from staining experiment (Total=31) were subsequently treated with sodium hypochlorite (NaOCl) and SEM images were observed. About 8.75% of HAs tested positive in bacterial culture; Streptococcus sanguis, Dermabacter hominis, Staphylococcus haemolyticus, and Aspergillus species were isolated. Phloxine B staining was positive for used and sterilized HAs when compared to controls. The SEM images revealed deposits in the used HAs and although treatment with NaOCl eliminated the contamination of cultured HAs, the SEM showed visible debris in the HA thread region. This in-vitro study concluded that SEM images showed debris in used HAs at screw-hole and thread regions even though they tested negative in bacterial culture. The treatment with NaOCl of used HAs showed no bacterial contamination but the debris was observed in SEM images. Future studies on the chemical composition, biological implications, and clinical influence is warranted before considering the reuse of HAs.

Sign in / Sign up

Export Citation Format

Share Document