Induction of delayed-type hypersensitivity to Leishmania major and the concomitant acceleration of disease development in progressive murine cutaneous leishmaniasis.

Formalin-killed promastigotes (FKP) of Leishmania major, in combination with Montanide ISA 720 (MISA), BCG or alum were used in vaccination of an inbred murine model against cutaneous leishmaniasis (CL). Significant and specific increases in anti-FKP IgG responses were detected for both alum-FKP and BCG-FKP compared to MISA-FKP (p < 0.001). Significant increases in splenic lymphocyte recall proliferation was obtained in the MISA-FKP vaccinated mice compared to alum-FKP or BCG-FKP vaccinated groups (p < 0.01). The highest interferon-γ responses were observed in the BCG-FKP group followed by the MISA-FKP while the alum-FKP gave the least responses. Significantly reduced lesion sizes were obtained in the MISA-FKP group compared to the BCG/alum adjuvants-FKP vaccinated groups. Although the BCG-FKP group showed the highest IFN-γ responses, it failed to control cutaneous lesions. Significant reductions in parasite numbers were observed in the MISA-FKP and BCG-FKP vaccinated groups (p < 0.001). There was a good correlation between parasite burden and IFN-γ level indicating IFN-γ response as a sensitive parameter of the immune status. In conclusion, MISA-FKP is the most efficacious vaccine formulation against murine cutaneous leishmaniasis.

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IFN-gamma and Delayed-Type Hypersensitivity are Associated with Cutaneous Leishmaniasis in Vervet Monkeys following Secondary Rechallenge with Leishmania major

Scandinavian Journal of Immunology ◽

10.1111/j.1365-3083.1992.tb01618.x ◽

1992 ◽

Vol 36 (s1) ◽

pp. 48-52 ◽

Cited By ~ 15

Author(s):

J. O. OLOBO ◽

G. D. F. REID ◽

J. I. GITHURE ◽

C. O. ANJILI

Keyword(s):

Cutaneous Leishmaniasis ◽

Leishmania Major ◽

Delayed Type Hypersensitivity ◽

Vervet Monkeys ◽

Ifn Gamma

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An α-Gal-containing neoglycoprotein-based vaccine partially protects against murine cutaneous leishmaniasis caused by Leishmania major

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0006039 ◽

2017 ◽

Vol 11 (10) ◽

pp. e0006039 ◽

Cited By ~ 27

Author(s):

Eva Iniguez ◽

Nathaniel S. Schocker ◽

Krishanthi Subramaniam ◽

Susana Portillo ◽

Alba L. Montoya ◽

...

Keyword(s):

Cutaneous Leishmaniasis ◽

Leishmania Major ◽

Murine Cutaneous Leishmaniasis

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Effects of qinghaosu (artemisinin) and its derivatives on experimental cutaneous leishmaniasis

Parasitology ◽

10.1017/s0031182000074758 ◽

1993 ◽

Vol 106 (1) ◽

pp. 7-11 ◽

Cited By ~ 64

Author(s):

D. M. Yang ◽

F. Y. Liew

Keyword(s):

Effective Dose ◽

Cutaneous Leishmaniasis ◽

Intravenous Injection ◽

Leishmania Major ◽

Disease Development ◽

Spleen Cells ◽

Murine Macrophages ◽

Normal Spleen

SUMMARYWe have investigated the effect of qinghaosu (QHS, artemisinin) and its derivatives on Leishmania major replication in vitro and on the disease development in mice infected with L. major. Artemisinin is effective against promastigotes in vitro, with an ED 50 (50% effective dose) at 7.5 × 10−7 m. Both artemisinin and artemether are leishmanicidal for amastigotes in infected murine macrophages in vitro, with ED50 at 3 × 10−5 m and 3 × 10−6 m respectively. These compounds have no effect on the viability of macrophages or on the phytohaemaglutinin-induced proliferation of normal spleen cells, even at 10−4 m. BALB/c mice infected in the footpad with L. major developed significantly smaller lesions and parasite loads when treated with the compounds. Intra-lesion injection of the compounds was the most effective route. The intramuscular and oral routes were also effective; however, intravenous injection with artesunate was not effective.

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