scholarly journals The 102-Kilobase pgm Locus of Yersinia pestis: Sequence Analysis and Comparison of Selected Regions among Different Yersinia pestis and Yersinia pseudotuberculosis Strains

1999 ◽  
Vol 67 (9) ◽  
pp. 4851-4861 ◽  
Author(s):  
Carmen Buchrieser ◽  
Christophe Rusniok ◽  
Lionel Frangeul ◽  
Elisabeth Couve ◽  
Alain Billault ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Yersinia Pestis ◽  
Gene Cluster ◽  
Yersinia Pseudotuberculosis ◽  
Iron Acquisition ◽  
Regulatory System ◽  
Insertion Element ◽  
En Bloc ◽  
Sequence Comparisons ◽  
Flanking Regions

ABSTRACT We report the complete 119,443-bp sequence of the pgmlocus from Yersinia pestis and its flanking regions. Sequence analysis confirms that the 102-kb unstable pgmlocus is composed of two distinct parts: the pigmentation segment and a high-pathogenicity island (HPI) which carries virulence genes involved in iron acquisition (yersiniabactin biosynthetic gene cluster). Within the HPI, three genes coding for proteins related to phage proteins were uncovered. They are located at both extremities indicating that the entire HPI was acquired en bloc by phage-mediated horizontal transfer. We identified, within the pigmentation segment, two novel loci that may be involved in virulence: a fimbriae gene cluster and a locus probably encoding a two component regulatory system similar to the BvgAS regulatory system of Bordetella pertussis. Three genes containing frameshift mutations and two genes interrupted by insertion element insertion were found within this region. To investigate diversity among different Y. pestis and Yersinia pseudotuberculosis strains, the sequence of selected regions of the pgm locus and flanking regions were compared from 20 different Y. pestis and 10 Y. pseudotuberculosis strains. The results showed that the genes interrupted in Y. pestis are intact in Y. pseudotuberculosis. However, one of these mutations, in thebvgS homologue, is only present in Y. pestisstrains of biovar Orientalis and not in those of the biovars Antiqua and Medievalis. The results obtained by analysis of variable positions in the sequence are in accordance with historical records, confirming that biovar Orientalis is the most recent lineage. Furthermore, sequence comparisons among 29 Yersinia strains suggest thatY. pestis is a recently emerged pathogen that is probably entering the initial phase of reductive evolution.

Sequence analysis of the yadA, inv, and ail genes and their expression in the main and nonmain Yersinia pestis subspecies and Yersinia pseudotuberculosis

2010 ◽  
Vol 46 (6) ◽  
pp. 645-651 ◽  
Author(s):  
G. A. Eroshenko ◽  
G. N. Odinokov ◽  
L. M. Kukleva ◽  
Ya. M. Krasnov ◽  
V. V. Kutyrev
Keyword(s):  
Sequence Analysis ◽  
Yersinia Pestis ◽  
Yersinia Pseudotuberculosis

scholarly journals cDNA cloning and sequence analysis of human pancreatic procarboxypeptidase A1

1992 ◽  
Vol 287 (1) ◽  
pp. 299-303 ◽  
Author(s):  
L Catasús ◽  
V Villegas ◽  
R Pascual ◽  
F X Avilés ◽  
C Wicker-Planquart ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Amino Acids ◽  
Amino Acid ◽  
Sequence Analysis ◽  
Central Region ◽  
Polyclonal Antibodies ◽  
Amino Acid Sequences ◽  
Sequence Comparisons ◽  
Activation Segment ◽  
Flanking Regions

Using polyclonal antibodies raised against human pancreatic procarboxypeptidases, a full-length cDNA coding for an A-type proenzyme was isolated from a lambda gt11 human pancreatic library. This cDNA contains standard 3′ and 5′ flanking regions, a poly(A)+ tail and a central region of 1260 nucleotides coding for a protein of 419 amino acids. On the basis of sequence comparisons, the human protein was classified as a procarboxypeptidase A1 which is very similar to the previously described A1 forms from rat and bovine pancreatic glands. The presence of the amino acid sequences assumed to be of importance for the zymogen inhibition by its activation segment, primarily on the basis of the recently reported crystal structure of the B form, further supports the proposed classification.

scholarly journals The importance of the magnesium transporter MgtB for virulence of Yersinia pseudotuberculosis and Yersinia pestis

Microbiology ◽  
2014 ◽  
Vol 160 (12) ◽  
pp. 2710-2717 ◽  
Author(s):  
Donna C. Ford ◽  
George W. P. Joshua ◽  
Brendan W. Wren ◽  
Petra C. F. Oyston
Keyword(s):  
Yersinia Pestis ◽  
Yersinia Pseudotuberculosis ◽  
Regulatory System ◽  
Transport Systems ◽  
Magnesium Ion ◽  
Gram Negative Bacteria ◽  
Two Component ◽  
Respective Parent ◽  
Magnesium Transporter ◽  
Suitable Target

Mg2+ has been shown to be an important signal controlling gene regulation via the PhoPQ two-component regulatory system for a range of Gram-negative bacteria, including Yersinia pestis and Yersinia pseudotuberculosis. The magnesium ion transporter MgtB is part of the complex PhoPQ regulon, being upregulated in response to low Mg2+. Despite the presence of other Mg2+ transport systems in Yersinia, inactivation of mgtB had a significant effect on the ability of the bacteria to scavenge this crucial ion. Whereas inactivation of PhoPQ is reported to adversely affect intracellular survival, we show that Y. pestis and Y. pseudotuberculosis ΔmgtB mutants survived equally as well as the respective parent strain within macrophages, although they were more sensitive to killing in the Galleria model of infection. Surprisingly, despite MgtB being only one member of the Mg2+ stimulon and PhoPQ controlling the expression levels of a range of genes including mgtB, the Yersinia ΔmgtB mutants were more highly attenuated than the equivalent Yersinia ΔphoP mutants in mouse models of infection. MgtB may be a suitable target for development of novel antimicrobials, and investigation of its role may help elucidate the contribution of this component of the PhoPQ regulon to pathogenesis.

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