scholarly journals Localization of Spirochetes with the Structural Characteristics of Treponema hyodysenteriae in the Lesions of Swine Dysentery

1974 ◽  
Vol 9 (1) ◽  
pp. 167-178 ◽  
Author(s):  
R. D. Glock ◽  
D. L. Harris ◽  
J. P. Kluge
Keyword(s):  
Structural Characteristics ◽  
Swine Dysentery ◽  
Treponema Hyodysenteriae

scholarly journals Studies on the Pathogenesis of Swine Dysentery

1979 ◽  
Vol 16 (4) ◽  
pp. 450-465 ◽  
Author(s):  
B. P. Wilcock ◽  
H. J. Olander
Keyword(s):  
Volatile Fatty Acids ◽  
Cell Hyperplasia ◽  
Swine Dysentery ◽  
India Ink ◽  
Perfusion Studies ◽  
Colony Forming Units ◽  
Pure Cultures ◽  
Treponema Hyodysenteriae ◽  
Colonic Secretion ◽  
Mucosal Necrosis

Swine dysentery was induced in pigs and in ligated colonic segments by inoculation of pure cultures of, or colonic contents containing, Treponema hyodysenteriae. The mildest changes, best seen in ligated segments 48 or 72 hours after inoculation, were congestion and leucocytic margination in mucosal capillaries and depletion of mucigen from goblet cells lining the base of the crypts of Lieberkühn. Superficial mucosal necrosis and crypt cell hyperplasia were later changes. Perfusion studies with India ink did not demonstrate occlusive mucosal ischemia in acute swine dysentery. Mucosa with lesions of swine dysentery contained at least 105 colony forming units of T. hyodysenteriae per gram. Mucosa without lesions had 105 or fewer T. hyodysenteriae per gram. Segments with acute swine dysentery were distended with clear mucoid fluid with electrolyte composition indicative of net colonic secretion. No increase in the concentration of volatile fatty acids was detected in content from intact colons or colonic segments with lesions of acute swine dysentery.

scholarly journals Studies on the Pathogenesis of Swine Dysentery

1979 ◽  
Vol 16 (5) ◽  
pp. 567-573 ◽  
Author(s):  
B. P. Wilcock ◽  
H. J. Olander
Keyword(s):  
Light Microscopy ◽  
Cell Cultures ◽  
Live Cells ◽  
Morphologic Change ◽  
Swine Dysentery ◽  
Visible Effect ◽  
Treponema Hyodysenteriae ◽  
Colonic Content

Broth cultures of Treponema hyodysenteriae and colonic content from pigs with swine dysentery were tested for cytotoxicity in cell cultures, erythrocyte suspensions and in ligated segments of pig colon. Live cells of T. hyodysenteriae attached to the surface of cells in all cultures tested but did not penetrate them nor cause morphologic change detectable by light microscopy. Only live T. hyodysenteriae caused erythrolysis. Broth cultures or colonic content sterilized by filtration or by disruption with ultrasound had no visible effect on the cell cultures, erythrocyte suspensions or the mucosa of ligated colonic segments.

Diagnosis of swine dysentery: spirochaetes which may be confused with Treponema hyodysenteriae

1976 ◽  
Vol 99 (25-26) ◽  
pp. 498-500 ◽  
Author(s):  
M. Hudson ◽  
T. Alexander ◽  
R. Lysons
Keyword(s):  
Swine Dysentery ◽  
Treponema Hyodysenteriae

scholarly journals A comparative study of spirochaetes from the porcine alimentary tract

1981 ◽  
Vol 86 (2) ◽  
pp. 173-182 ◽  
Author(s):  
Ruth M. Lemcke ◽  
M. R. Burrows
Keyword(s):  
Alimentary Tract ◽  
Fluorescent Antibody ◽  
Electrophoretic Pattern ◽  
Antibody Test ◽  
Single Species ◽  
The Other ◽  
Serological Tests ◽  
Swine Dysentery ◽  
Single Strain ◽  
Treponema Hyodysenteriae

SUMMARYStrains ofTreponema hyodysenteriaecapable of inducing swine dysentery in specific pathogen-free pigs were compared with other spirochaetes from the porcine alimentary tract by biochemical and serological tests and by electrophoresis of their proteins. Carbohydrate fermentation and esculin hydrolysis were similar in all the spirochaetes. Indole was produced byT. hyodysenteriaeand by some of the other spirochaetes. Analysis of the fatty acids produced from glucose showed a difference betweenT. hyodysenteriaeand other spirochaetes only in the amount ofn-butyric acid produced. The indirect fluorescent antibody test showed extensive cross-reactions between all the spirochaetes unless antisera were first absorbed. A microtitre agglutination test and a growth-inhibition test were both more specific; strains ofT. hyodysenteriaecould be distinguished from the other spirochaetes using unabsorbed sera. Both tests revealed some antigenic heterogeneity among strains ofT. hyodysenteriae. The cell proteins of a single strain ofT. hyodysenteriaegave an electrophoretic pattern distinct from those of the other spirochaetes.Two of the six spirochaetes not associated with swine dysentery, PWS/B and PWS/C, were indistinguishable serologically and electrophoretically. The other four strains were serologically distinct from one another and from PWS/B and PWS/C. Only two of these spirochaetes were examined electrophoretically, but each gave a different pattern from PWS/B and PWS/C. The diversity observed among spirochaetes not associated with swine dysentery indicates that their suggested inclusion in a single species,T. innocens, may prove to be unjustified.

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