scholarly journals Studies on the Pathogenesis of Swine Dysentery

1979 ◽  
Vol 16 (5) ◽  
pp. 567-573 ◽  
Author(s):  
B. P. Wilcock ◽  
H. J. Olander
Keyword(s):  
Light Microscopy ◽  
Cell Cultures ◽  
Live Cells ◽  
Morphologic Change ◽  
Swine Dysentery ◽  
Visible Effect ◽  
Treponema Hyodysenteriae ◽  
Colonic Content

Broth cultures of Treponema hyodysenteriae and colonic content from pigs with swine dysentery were tested for cytotoxicity in cell cultures, erythrocyte suspensions and in ligated segments of pig colon. Live cells of T. hyodysenteriae attached to the surface of cells in all cultures tested but did not penetrate them nor cause morphologic change detectable by light microscopy. Only live T. hyodysenteriae caused erythrolysis. Broth cultures or colonic content sterilized by filtration or by disruption with ultrasound had no visible effect on the cell cultures, erythrocyte suspensions or the mucosa of ligated colonic segments.

scholarly journals Studies on the Pathogenesis of Swine Dysentery

1979 ◽  
Vol 16 (4) ◽  
pp. 450-465 ◽  
Author(s):  
B. P. Wilcock ◽  
H. J. Olander
Keyword(s):  
Volatile Fatty Acids ◽  
Cell Hyperplasia ◽  
Swine Dysentery ◽  
India Ink ◽  
Perfusion Studies ◽  
Colony Forming Units ◽  
Pure Cultures ◽  
Treponema Hyodysenteriae ◽  
Colonic Secretion ◽  
Mucosal Necrosis

Swine dysentery was induced in pigs and in ligated colonic segments by inoculation of pure cultures of, or colonic contents containing, Treponema hyodysenteriae. The mildest changes, best seen in ligated segments 48 or 72 hours after inoculation, were congestion and leucocytic margination in mucosal capillaries and depletion of mucigen from goblet cells lining the base of the crypts of Lieberkühn. Superficial mucosal necrosis and crypt cell hyperplasia were later changes. Perfusion studies with India ink did not demonstrate occlusive mucosal ischemia in acute swine dysentery. Mucosa with lesions of swine dysentery contained at least 105 colony forming units of T. hyodysenteriae per gram. Mucosa without lesions had 105 or fewer T. hyodysenteriae per gram. Segments with acute swine dysentery were distended with clear mucoid fluid with electrolyte composition indicative of net colonic secretion. No increase in the concentration of volatile fatty acids was detected in content from intact colons or colonic segments with lesions of acute swine dysentery.

scholarly journals AI-powered transmitted light microscopy for functional analysis of live cells

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Dongyoung Kim ◽  
Yoohong Min ◽  
Jung Min Oh ◽  
Yoon-Kyoung Cho
Keyword(s):  
Functional Analysis ◽  
Light Microscopy ◽  
Cell Tracking ◽  
Transmitted Light ◽  
Live Cells ◽  
Structure Identification ◽  
Maturation Stage ◽  
Native Form ◽  
Subcellular Organelles ◽  
Transmitted Light Microscopy

AbstractTransmitted light microscopy can readily visualize the morphology of living cells. Here, we introduce artificial-intelligence-powered transmitted light microscopy (AIM) for subcellular structure identification and labeling-free functional analysis of live cells. AIM provides accurate images of subcellular organelles; allows identification of cellular and functional characteristics (cell type, viability, and maturation stage); and facilitates live cell tracking and multimodality analysis of immune cells in their native form without labeling.

scholarly journals Improved spatial resolution by induced live cell and organelle swelling in hypotonic solutions

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Astha Jaiswal ◽  
Christian H. Hoerth ◽  
Ana M. Zúñiga Pereira ◽  
Holger Lorenz
Keyword(s):  
Endoplasmic Reticulum ◽  
Cost Effectiveness ◽  
High Resolution ◽  
Light Microscopy ◽  
Matrix Protein ◽  
Super Resolution ◽  
Ease Of Use ◽  
Live Cells ◽  
Cell Organelles ◽  
Morphology Changes

Abstract Induced morphology changes of cells and organelles are by far the easiest way to determine precise protein sub-locations and organelle quantities in light microscopy. By using hypotonic solutions to swell mammalian cell organelles we demonstrate that precise membrane, lumen or matrix protein locations within the endoplasmic reticulum, Golgi and mitochondria can reliably be established. We also show the benefit of this approach for organelle quantifications, especially for clumped or intertwined organelles like peroxisomes and mitochondria. Since cell and organelle swelling is reversible, it can be applied to live cells for successive high-resolution analyses. Our approach outperforms many existing imaging modalities with respect to resolution, ease-of-use and cost-effectiveness without excluding any co-utilization with existing optical (super)resolution techniques.

Studies on the surfaces of cercariae and schistosomula of Schistosoma mansoni

Parasitology ◽  
1970 ◽  
Vol 61 (1) ◽  
pp. 127-134 ◽  
Author(s):  
J. R. Kusel
Keyword(s):  
Acetic Acid ◽  
Light Microscopy ◽  
Schistosoma Mansoni ◽  
Calcium Chloride ◽  
Physical Nature ◽  
Urea Solution ◽  
Chemical Changes ◽  
Visible Effect ◽  
The Stability

The physical nature of the cercarial surface has been observed by several workers to change during the penetration of the cercaria through the skin and its metamorphosis into the schistosomulum. Very little is known about the chemistry of this change. This paper reports the effects of the treatment of the surfaces of cercariae and schistosomula with various reagents as assessed by light microscopy. It was found that there were great differences in the stability of the surfaces of these parasitic forms. The schistosomular surface was stable to 0·2 m acetic acid, 5% calcium chloride and 8 m urea, whereas the cercarial surface was affected by these reagents. 8 m urea completely dissolved the cercarial surface, while it had little visible effect on that of the schistosomulum. This dissolution of the cercarial surface could be prevented by including small quantities of sodium or calcium chloride in the 8 m urea solution. A method has been developed to isolate pure preparations of cercarial and schistosomular surfaces. Hypotheses about the chemical changes in the surface during metamorphosis should be amenable to investigation using this method.

Sign in / Sign up

Export Citation Format

Share Document