scholarly journals Homogeneity of Envelope Proteins of Escherichia coli Separated by Gel Electrophoresis in Sodium Dodecyl Sulfate

1973 ◽  
Vol 113 (1) ◽  
pp. 304-312 ◽  
Author(s):  
Masayori Inouye ◽  
Mei-Lan Yee
Keyword(s):  
Escherichia Coli ◽  
Sodium Dodecyl Sulfate ◽  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Envelope Proteins ◽  
Dodecyl Sulfate

scholarly journals mraY Is an Essential Gene for Cell Growth in Escherichia coli

1998 ◽  
Vol 180 (23) ◽  
pp. 6429-6432 ◽  
Author(s):  
David S. Boyle ◽  
William D. Donachie
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Sodium Dodecyl Sulfate ◽  
Gel Electrophoresis ◽  
Essential Gene ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Dodecyl Sulfate

ABSTRACT The synthesis of the murein precursor lipid I is performed by MraY. We have shown that mraY is an essential gene for cell growth. Cells depleted of MraY first swell and then lyse. The expression of mraY DNA in vitro produces a 40-kDa polypeptide detectable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

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