scholarly journals Identification and characterization of the Rhizobium meliloti ntrC gene: R. meliloti has separate regulatory pathways for activation of nitrogen fixation genes in free-living and symbiotic cells.

1987 ◽  
Vol 169 (4) ◽  
pp. 1423-1432 ◽  
Author(s):  
W W Szeto ◽  
B T Nixon ◽  
C W Ronson ◽  
F M Ausubel
Genome ◽  
1989 ◽  
Vol 31 (1) ◽  
pp. 354-360 ◽  
Author(s):  
San Chiun Shen ◽  
Shui Ping Wang ◽  
Guan Qiao Yu ◽  
Jia Bi Zhu

Genes that specify nodulation (nod genes) are only active in the free-living rhizobia or in the nodule initiation state of rhizobia. As soon as the repression of nod genes occurs in the bacteroids of the nodule, nifA is induced, while ntrC is inactivated and thus the nifA-mediated nif/fix genes are turned on. Limitation of available oxygen brings about the induction of nifA, which reflects the actual status of nif/fix gene activities in symbiotic state of rhizobia. Oxygen thus appears to be a major symbiotic signal to the expression of bacteroid nif/fix genes. Mutation of nifA or shortage of nifA product in wild-type rhizobia caused by the inhibition of multicopy nifH/fixA promoters leads to an abnormal development of nodules and premature degradation of bacteroids in nodules.Key words: nitrogen fixation, nodulation, nif/fix regulation, nifA mutant.


2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Frances Patricia Jones ◽  
Ian M. Clark ◽  
Robert King ◽  
Liz J. Shaw ◽  
Martin J. Woodward ◽  
...  

1987 ◽  
Vol 33 (4) ◽  
pp. 314-321 ◽  
Author(s):  
Roger Wheatcroft ◽  
Robert J. Watson

ISRm1, an insertion sequence present in Rhizobium meliloti strain 1021, has been identified as the cause of the Nod− phenotypes in two mutants of another strain, JJ1c10. The insertions were found to be at different sites, though only about 100 base pairs apart within the nodC gene. ISRm1 causes no mutations in the nifHDK gene region of strain JJ1c10, as it does at high frequency in strain 1021. In JJ1c10 ISRm1 inserted at high frequency into a region of the genome adjacent to copies of other reiterated DNA segments. The target region was not required for symbiotic nitrogen fixation.


1982 ◽  
Vol 188 (3) ◽  
pp. 433-439 ◽  
Author(s):  
A. Kondorosi ◽  
E. Kondorosi ◽  
C. E. Pankhurst ◽  
W. J. Broughton ◽  
Z. Banfalvi

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