Identification and characterization of insertion sequence ISRm1 in Rhizobium meliloti JJ1c10

1987 ◽  
Vol 33 (4) ◽  
pp. 314-321 ◽  
Author(s):  
Roger Wheatcroft ◽  
Robert J. Watson
Keyword(s):  
High Frequency ◽  
Insertion Sequence ◽  
Symbiotic Nitrogen Fixation ◽  
Rhizobium Meliloti ◽  
Base Pairs ◽  
Target Region ◽  
Meliloti Strain ◽  
Nodc Gene ◽  
Identification And Characterization

ISRm1, an insertion sequence present in Rhizobium meliloti strain 1021, has been identified as the cause of the Nod− phenotypes in two mutants of another strain, JJ1c10. The insertions were found to be at different sites, though only about 100 base pairs apart within the nodC gene. ISRm1 causes no mutations in the nifHDK gene region of strain JJ1c10, as it does at high frequency in strain 1021. In JJ1c10 ISRm1 inserted at high frequency into a region of the genome adjacent to copies of other reiterated DNA segments. The target region was not required for symbiotic nitrogen fixation.

scholarly journals Identification and characterization of Fep15, a new selenocysteine-containing member of the Sep15 protein family

2006 ◽  
Vol 394 (3) ◽  
pp. 575-579 ◽  
Author(s):  
Sergey V. Novoselov ◽  
Deame Hua ◽  
Alexey V. Lobanov ◽  
Vadim N. Gladyshev
Keyword(s):  
Mammalian Cells ◽  
Insertion Sequence ◽  
Phylogenetic Analyses ◽  
Dependent Manner ◽  
Putative Active Site ◽  
A Genome ◽  
Sequence Elements ◽  
Identification And Characterization ◽  
Insertion Sequence Elements

Sec (selenocysteine) is a rare amino acid in proteins. It is co-translationally inserted into proteins at UGA codons with the help of SECIS (Sec insertion sequence) elements. A full set of selenoproteins within a genome, known as the selenoproteome, is highly variable in different organisms. However, most of the known eukaryotic selenoproteins are represented in the mammalian selenoproteome. In addition, many of these selenoproteins have cysteine orthologues. Here, we describe a new selenoprotein, designated Fep15, which is distantly related to members of the 15 kDa selenoprotein (Sep15) family. Fep15 is absent in mammals, can be detected only in fish and is present in these organisms only in the selenoprotein form. In contrast with other members of the Sep15 family, which contain a putative active site composed of Sec and cysteine, Fep15 has only Sec. When transiently expressed in mammalian cells, Fep15 incorporated Sec in an SECIS- and SBP2 (SECIS-binding protein 2)-dependent manner and was targeted to the endoplasmic reticulum by its N-terminal signal peptide. Phylogenetic analyses of Sep15 family members suggest that Fep15 evolved by gene duplication.

Identification and characterization of a bipotent (erythroid and megakaryocytic) cell precursor from the spleen of phenylhydrazine-treated mice

Blood ◽  
2000 ◽  
Vol 95 (8) ◽  
pp. 2559-2568 ◽  
Author(s):  
Alessandro Maria Vannucchi ◽  
Francesco Paoletti ◽  
Silvia Linari ◽  
Cristina Cellai ◽  
Roberto Caporale ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Hemolytic Anemia ◽  
Cell Population ◽  
High Frequency ◽  
Cell Precursor ◽  
A Cell ◽  
Identification And Characterization ◽  
Megakaryocytic Cell

Abstract We have identified a cell population expressing erythroid (TER-119) and megakaryocyte (4A5) markers in the bone marrow of normal mice. This population is present at high frequency in the marrows and in the spleens involved in the erythroid expansion that occurs in mice recovering from phenylhydrazine (PHZ)-induced hemolytic anemia. TER-119+/4A5+ cells were isolated from the spleen of PHZ-treated animals and were found to be blast-like benzidine-negative cells that generate erythroid and megakaryocytic cells within 24-48 hours of culture in the presence of erythropoietin (EPO) or thrombopoietin (TPO). TER-119+/4A5+ cells represent a late bipotent erythroid and megakaryocytic cell precursors that may exert an important role in the recovery from PHZ-induced anemia.

scholarly journals Identification and characterization of functional genes encoding the mouse major urinary proteins.

1987 ◽  
Vol 7 (10) ◽  
pp. 3705-3712 ◽  
Author(s):  
W A Held ◽  
J F Gallagher ◽  
C M Hohman ◽  
N J Kuhn ◽  
B M Sampsell ◽  
...  
Keyword(s):  
Tissue Specificity ◽  
Urinary Proteins ◽  
Base Pairs ◽  
Rich Region ◽  
Male And Female ◽  
Major Urinary Proteins ◽  
Genes Encoding ◽  
Flanking Region ◽  
Identification And Characterization

Mouse Ltk- cells were stably transfected with cloned genes encoding the mouse major urinary proteins (MUPs). C57BL/6J MUP genomic clones encoding MUP 2 (BL6-25 and BL6-51), MUP 3 (BL6-11 and BL6-3), and MUP 4 (BL6-42) have been identified. In C57BL/6J mice, MUP 2 and MUP 4 are known to be synthesized in male, but not female, liver, and MUP 3 is known to be synthesized in both male and female liver and mammary gland. A BALB/c genomic clone (BJ-31) was shown to encode a MUP that is slightly more basic than MUP 2 and was previously shown to be synthesized in both male and female liver of BALB/c but not C57BL/6 mice. Comigration on two-dimensional polyacrylamide gels of the MUPs encoded by the transfecting gene provides a basis for tentative identification of the tissue specificity and mode of regulation of each gene. DNA sequence analysis of the 5' flanking region indicates that the different MUP genes are highly homologous (0.20 to 2.40% divergence) within the 879 base pairs analyzed. The most prominent differences in sequence occur within an A-rich region just 5' of the TATA box. This region (from -47 to -93) contains primarily A or C(A)N nucleotides and varies from 15 to 46 nucleotides in length in the different clones.

Identification and characterization of IS1476, an insertion sequence-like element that disrupts VanY function in a vancomycin-resistant Enterococcus faecium strain.

1997 ◽  
Vol 41 (8) ◽  
pp. 1805-1807 ◽  
Author(s):  
M G MacKinnon ◽  
M A Drebot ◽  
G J Tyrrell
Keyword(s):  
Resistance Gene ◽  
Enterococcus Faecium ◽  
Insertion Sequence ◽  
Vancomycin Resistant Enterococcus ◽  
Detection Strategy ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Identification And Characterization ◽  
Faecium Strain

The vanY gene of vancomycin-resistant enterococci encodes a D,D-carboxypeptidase. By using a PCR detection strategy, a VanA Enterococcus faecium clinical isolate was found to have an insertion sequence (IS)-like element designated IS1476 in vanY. The activity of the VanY D,D-carboxypeptidase in this isolate was decreased in a fluorometric fluoraldehyde o-phthalaldehyde assay with diacetyl-L-Lys-D-Ala-D-Ala as the substrate. This, to our knowledge, is the first report of an IS-like element in a vancomycin resistance gene.

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