scholarly journals Isolation of the second Bacillus thuringiensis RNA polymerase that transcribes from a crystal protein gene promoter.

1990 ◽  
Vol 172 (12) ◽  
pp. 6682-6688 ◽  
Author(s):  
K L Brown ◽  
H R Whiteley
Keyword(s):  
Bacillus Thuringiensis ◽  
Rna Polymerase ◽  
Protein Gene ◽  
Gene Promoter ◽  
Crystal Protein

Expression of a Bacillus thuringiensis crystal protein gene in Pseudomonas cepacia 526

1990 ◽  
Vol 36 (12) ◽  
pp. 879-884 ◽  
Author(s):  
Carolyn A. Stock ◽  
Thomas J. McLoughlin ◽  
Janet A. Klein ◽  
Michael J. Adang
Keyword(s):  
Bacillus Thuringiensis ◽  
Manduca Sexta ◽  
Protein Gene ◽  
Crystal Protein ◽  
Pseudomonas Cepacia ◽  
Broad Host Range ◽  
Cry Protein ◽  
Insecticidal Crystal Protein ◽  
Tobacco Plants ◽  
Range Vector

An insecticidal crystal protein gene, cryIA(C), from Bacillus thuringiensis HD-1 was cloned into a broad host range vector, pSUP204, and the resulting plasmid, pSUP89A, was conjugated into a plant-colonizing bacterium, Pseudomonas cepacia 526. Southern blot analysis detected the presence of the cry gene in Pseudomonas cepacia 526 transconjugants. Production of a truncated insecticidal crystal protein in Pseudomonas cepacia was detected by Western blotting and ELISA techniques and was found to have insecticidal activity against the tobacco hornworm, Manduca sexta, in an artificial diet assay. We also followed the establishment of the Pseudomonas cepacia 526/Bt transconjugant on axenically grown tobacco plants and found that approximately 1% of the Pseudomonas cells retained pSUP89A 3 days after application. In spite of this instability, treated tobacco plants were protected from Manduca sexta infestation. Key words: Bacillus thuringiensis, Pseudomonas cepacia, insecticidal crystal protein, Cry protein.

scholarly journals Cloning and Expression of an Insecticidal k-73 Type Crystal Protein Gene from Bacillus thuringiensis var. kurstaki into Escherichia coli

1985 ◽  
Vol 50 (3) ◽  
pp. 623-628 ◽  
Author(s):  
James H. McLinden ◽  
Josanne R. Sabourin ◽  
Burton D. Clark ◽  
Diana R. Gensler ◽  
Wesley E. Workman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacillus Thuringiensis ◽  
Protein Gene ◽  
Crystal Protein ◽  
Cloning And Expression ◽  
Type Crystal
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