scholarly journals Molecular cloning and characterization of two genes encoding sigma factors that direct transcription from a Bacillus thuringiensis crystal protein gene promoter.

1991 ◽  
Vol 173 (12) ◽  
pp. 3846-3854 ◽  
Author(s):  
L F Adams ◽  
K L Brown ◽  
H R Whiteley
Keyword(s):  
Bacillus Thuringiensis ◽  
Molecular Cloning ◽  
Protein Gene ◽  
Gene Promoter ◽  
Sigma Factors ◽  
Crystal Protein ◽  
Direct Transcription ◽  
Genes Encoding

Molecular cloning and characterization of a novel SNAP25-type protein gene OsSNAP32 in rice (Oryza sativa L.)

2007 ◽  
Vol 35 (2) ◽  
pp. 145-152 ◽  
Author(s):  
Yong-Mei Bao ◽  
Jian-Fei Wang ◽  
Ji Huang ◽  
Hong-Sheng Zhang
Keyword(s):  
Oryza Sativa ◽  
Molecular Cloning ◽  
Protein Gene ◽  
Oryza Sativa L ◽  
Type Protein

scholarly journals New Strategy for Identification of Novel cry-Type Genes from Bacillus thuringiensis Strains

2005 ◽  
Vol 71 (2) ◽  
pp. 761-765 ◽  
Author(s):  
Corina M. Berón ◽  
Leonardo Curatti ◽  
Graciela L. Salerno
Keyword(s):  
Bacillus Thuringiensis ◽  
Phylogenetic Distance ◽  
Cloning And Sequencing ◽  
Degenerate Primers ◽  
Dna Templates ◽  
Cry Proteins ◽  
New Strategy ◽  
Genes Encoding ◽  
Encoding Protein

ABSTRACT We designed five degenerate primers for detection of novel cry genes from Bacillus thuringiensis strains. An efficient strategy was developed based on a two-step PCR approach with these primers in five pair combinations. In the first step, only one of the primer pairs is used in the PCR, which allows amplification of DNA fragments encoding protein regions that include consensus domains of representative proteins belonging to different Cry groups. A second PCR is performed by using the first-step amplification products as DNA templates and the set of five primer combinations. Cloning and sequencing of the last-step amplicons allow both the identification of known cry genes encoding Cry proteins covering a wide phylogenetic distance and the detection and characterization of cry-related sequences from novel B. thuringiensis isolates.

scholarly journals Molecular cloning and characterization of the genes encoding a microsomal oleate Δ12 desaturase (CsFAD2) and linoleate Δ15 desaturase (CsFAD3) from Camelina sativa

2016 ◽  
Vol 89 ◽  
pp. 405-415 ◽  
Author(s):  
Manuel Fernando Rodríguez-Rodríguez ◽  
Joaquín J. Salas ◽  
Mónica Venegas-Calerón ◽  
Rafael Garcés ◽  
Enrique Martínez-Force
Keyword(s):  
Molecular Cloning ◽  
Camelina Sativa ◽  
Genes Encoding ◽  
Δ12 Desaturase
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