scholarly journals Analysis by fluorescence microscopy of the development of compartment-specific gene expression during sporulation of Bacillus subtilis.

1994 ◽  
Vol 176 (10) ◽  
pp. 2898-2905 ◽  
Author(s):  
J E Bylund ◽  
L Zhang ◽  
M A Haines ◽  
M L Higgins ◽  
P J Piggot
Keyword(s):  
Gene Expression ◽  
Bacillus Subtilis ◽  
Fluorescence Microscopy ◽  
Specific Gene ◽  
Specific Gene Expression

scholarly journals Dual-Specificity Anti-sigma Factor Reinforces Control of Cell-Type Specific Gene Expression in Bacillus subtilis

PLoS Genetics ◽  
2015 ◽  
Vol 11 (4) ◽  
pp. e1005104 ◽  
Author(s):  
Mónica Serrano ◽  
JinXin Gao ◽  
João Bota ◽  
Ashley R. Bate ◽  
Jeffrey Meisner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bacillus Subtilis ◽  
Sigma Factor ◽  
Specific Gene ◽  
Cell Type ◽  
Specific Gene Expression ◽  
Dual Specificity ◽  
Cell Type Specific

scholarly journals Establishment of Prespore-Specific Gene Expression inBacillus subtilis: Localization of SpoIIE Phosphatase and Initiation of Compartment-Specific Proteolysis

1998 ◽  
Vol 180 (13) ◽  
pp. 3276-3284 ◽  
Author(s):  
Peter J. Lewis ◽  
Ling Juan Wu ◽  
Jeffery Errington
Keyword(s):  
Gene Expression ◽  
Bacillus Subtilis ◽  
Sigma Factor ◽  
Mother Cell ◽  
Immunofluorescence Microscopy ◽  
The Other ◽  
Specific Gene ◽  
Specific Gene Expression

ABSTRACT Immunofluorescence microscopy was used to study the establishment of compartment-specific transcription during sporulation inBacillus subtilis. Analysis of the distribution of the anti-anti-sigma factor, SpoIIAA, in a variety of mutant backgrounds supports a model in which the SpoIIE phosphatase, which activates SpoIIAA by dephosphorylation, is sequestered onto the prespore face of the asymmetric septum. Thus, prespore-specific gene expression apparently arises as a result of the compartmentalization of SpoIIE protein. The results also suggest the existence of at least two compartment-specific programs of proteolysis, one dependent on the mother cell-specific sigma factor ςE and the other dependent on the prespore-specific sigma factor ςF.

Sign in / Sign up

Export Citation Format

Share Document