scholarly journals Variation in the Effectors of the Type III Secretion System among Photorhabdus Species as Revealed by Genomic Analysis

2004 ◽  
Vol 186 (13) ◽  
pp. 4376-4381 ◽  
Author(s):  
Karine Brugirard-Ricaud ◽  
Alain Givaudan ◽  
Julian Parkhill ◽  
Noel Boemare ◽  
Frank Kunst ◽  
...  
Keyword(s):  
Related Species ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Genomic Analysis ◽  
Secretion System ◽  
Photorhabdus Luminescens ◽  
Type Iii ◽  
The Core ◽  
Entomopathogenic Bacteria ◽  
Core Components

ABSTRACT Entomopathogenic bacteria of the genus Photorhabdus harbor a type III secretion system. This system was probably acquired prior to the separation of the species within this genus. Furthermore, the core components of the secretion machinery are highly conserved but the predicted effectors differ between Photorhabdus luminescens and P. asymbiotica, two highly related species with different hosts.

scholarly journals Structure of the core of the type III secretion system export apparatus

2018 ◽  
Vol 25 (7) ◽  
pp. 583-590 ◽  
Author(s):  
Lucas Kuhlen ◽  
Patrizia Abrusci ◽  
Steven Johnson ◽  
Joseph Gault ◽  
Justin Deme ◽  
...  
Keyword(s):  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Type Iii ◽  
The Core

Site-specific antiphagocytic function of the Photorhabdus luminescens type III secretion system during insect colonization

2005 ◽  
Vol 7 (3) ◽  
pp. 363-371 ◽  
Author(s):  
Karine Brugirard-Ricaud ◽  
Eric Duchaud ◽  
Alain Givaudan ◽  
Pierre Alain Girard ◽  
Frank Kunst ◽  
...  
Keyword(s):  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Photorhabdus Luminescens ◽  
Site Specific ◽  
Type Iii

scholarly journals Author Correction: Structure of the core of the type III secretion system export apparatus

2018 ◽  
Vol 25 (8) ◽  
pp. 743-743 ◽  
Author(s):  
Lucas Kuhlen ◽  
Patrizia Abrusci ◽  
Steven Johnson ◽  
Joseph Gault ◽  
Justin Deme ◽  
...  
Keyword(s):  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Type Iii ◽  
The Core

scholarly journals Characterization of the SPI-1 Type III Secretion System in Pseudomonas fluorescens 2P24

2021 ◽  
Vol 12 ◽  
Author(s):  
Jing Wang ◽  
Yuan Luo ◽  
Yilin Gu ◽  
Hai-Lei Wei
Keyword(s):  
Pseudomonas Fluorescens ◽  
Gene Cluster ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Genomic Analysis ◽  
Gene Clusters ◽  
Secretion System ◽  
Type Iii ◽  
Plant Growth Promoting

Pseudomonas fluorescens 2P24 is a plant growth-promoting rhizobacterium (PGPR) isolated from wheat take-all decline soil. Genomic analysis of strain 2P24 revealed the presence of a complete SPI-1 type III secretion system (T3SS) gene cluster on the chromosome with an organization and orientation similar to the SPI-1 T3SS gene clusters of Salmonella enterica and P. kilonensis F113. Phylogenetic analysis revealed that the SPI-1 T3SS gene cluster of strain 2P24 might be obtained from Salmonella and Shigella by horizontal gene transfer. Two transcriptional regulator homologs of HilA and InvF were found from the SPI-1 T3SS gene cluster of strain 2P24. HilA regulated the expression of the structural genes positively, such as invG, sipB, sipD, prgI, and prgK. Prediction of transcriptional binding sites and RNA-seq analysis revealed 14 genes were up-regulated by InvF in strain 2P24. Exploring potential roles of SPI-1 T3SS revealed that it was not associated with motility. However, 2P24ΔinvF reduced resistance against Fusarium graminearum significantly. 2P24ΔhilA enhanced formation of biofilm significantly at 48 h. All three mutants 2P24ΔhilA, 2P24ΔinvF, and 2P24ΔinvE-C reduced the chemotactic responses to glucose significantly. Finally, the determination of SPI-1 mutants to trigger innate immunity in Nicotiana benthamiana showed that 2P24ΔinvE-C reduced the ability to induce the production of reactive oxygen species compared with the wild type strain 2P24.

Sign in / Sign up

Export Citation Format

Share Document