scholarly journals Improved Performance of Bacterium and Yeast Identification by a Commercial Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System in the Clinical Microbiology Laboratory

2011 ◽  
Vol 49 (9) ◽  
pp. 3441-3441 ◽  
Author(s):  
John Haigh ◽  
Amarjeet Degun ◽  
Melvyn Eydmann ◽  
Michael Millar ◽  
Mark Wilks
Keyword(s):  
Clinical Microbiology ◽  
Laser Desorption ◽  
Clinical Microbiology Laboratory ◽  
Laser Desorption Ionization ◽  
Microbiology Laboratory ◽  
Yeast Identification ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Mass Spectrometry System ◽  
Improved Performance

scholarly journals Phenotypic classification of multidrug-resistant Klebsiella through MALDI-TOF mass spectrometry

2015 ◽  
Vol 30 (1) ◽  
Author(s):  
Anna Di Taranto ◽  
Rosella De Nittis ◽  
Marco Fagioni ◽  
Tiziana Rollo ◽  
Paola Cella ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Multidrug Resistant ◽  
Clinical Microbiology Laboratory ◽  
Laser Desorption Ionization ◽  
Microbiology Laboratory ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Microbial Isolates

<em>Background</em>. Resistance to carbapenems in <em>Klebsiella</em> <em>pneumoniae</em> infections could compromise therapy efficiency in hospitals. In Italy, the percentage of <em>K</em>. <em>pmeumoniae</em> resistant to carbapenems blood-isolated is 35%. <br /><em>Materials</em> <em>and</em> <em>Methods</em>. Seventy-three multidrug-resistant <em>K</em>. <em>pneumoniae</em> clinical isolates coming from Foggia (Italy) have been phenotypically classified through matrix assisted laser desorption/ionization-time of flight mass spectrometry between February and May 2014 in order to locate an eventual infectious outbreak. <br /><em>Conclusions</em>. Classification of microbial isolates is essential to this purpose and starts from phenotypic methodologies to the most complex genotypic methods. Among these, mass spectrometry utilization appears to be a simple, useful and quick instrument in a clinical microbiology laboratory.

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