scholarly journals Comparison of Neo-Sensitabs Tablet Diffusion Assay with CLSI Broth Microdilution M38-A and Disk Diffusion Methods for Testing Susceptibility of Filamentous Fungi with Amphotericin B, Caspofungin, Itraconazole, Posaconazole, and Voriconazole

2008 ◽  
Vol 46 (5) ◽  
pp. 1793-1803 ◽  
Author(s):  
A. Espinel-Ingroff ◽  
E. Canton
Keyword(s):  
Amphotericin B ◽  
Filamentous Fungi ◽  
Disk Diffusion ◽  
Broth Microdilution ◽  
Testing Susceptibility ◽  
Diffusion Assay

scholarly journals Categorizing susceptibility of clinical isolates of Candida auris to amphotericin B, caspofungin, and fluconazole utilizing the CLSI M44-A2 disk diffusion method

2021 ◽  
Author(s):  
Natalie S. Nunnally ◽  
Tajah Damm ◽  
Shawn R. Lockhart ◽  
Elizabeth L. Berkow
Keyword(s):  
Amphotericin B ◽  
Accurate Method ◽  
Clinical Isolates ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Broth Microdilution ◽  
Candida Auris ◽  
Disk Diffusion Method ◽  
Microdilution Method ◽  
Broth Microdilution Method

We evaluated the CLSI M44ed3E disk diffusion method in comparison with the CLSI M27ed4 broth microdilution method for caspofungin and fluconazole and the Etest method for amphotericin B to categorize susceptibility of 347 clinical isolates of Candida auris. Utilizing the zone diameter cutoffs established here we observed the overall categorial agreement between the two methods. For caspofungin, concordant results were observed for 98% of isolates with <1% very major and 1% major errors. For fluconazole, concordant results were observed for 91% of isolates with 1% very major and 8% major errors. For amphotericin B, concordant results were observed for 74% of isolates with <1% very major errors and 25% major errors. The disk diffusion approach provides an accurate method for determining the susceptibility of C. auris for caspofungin and fluconazole, and for identification of at least 75% of amphotericin B-susceptible isolates.

scholarly journals Evaluation of Disk Diffusion Method for Determining Posaconazole Susceptibility of Filamentous Fungi: Comparison with CLSI Broth Microdilution Method

2006 ◽  
Vol 50 (3) ◽  
pp. 1108-1111 ◽  
Author(s):  
E. López-Oviedo ◽  
A. I. Aller ◽  
C. Martín ◽  
C. Castro ◽  
M. Ramirez ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Clinical Laboratory ◽  
Culture Media ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Broth Microdilution ◽  
Disk Diffusion Method ◽  
Microdilution Method ◽  
Broth Microdilution Method

ABSTRACT The disk diffusion method was evaluated for determining posaconazole susceptibility against 78 strains of molds using two culture media in comparison with the CLSI (Clinical Laboratory Standards Institute) broth microdilution method (M38-A). A significant correlation between disk diffusion and microdilution methods was observed with both culture media.

Comparison of in vitro antifungal susceptibilities of conidia and hyphae of filamentous fungi.

1997 ◽  
Vol 41 (12) ◽  
pp. 2760-2762 ◽  
Author(s):  
J Guarro ◽  
C Llop ◽  
C Aguilar ◽  
I Pujol
Keyword(s):  
Amphotericin B ◽  
Filamentous Fungi ◽  
Broth Microdilution ◽  
Opportunistic Fungi ◽  
Microdilution Method ◽  
Broth Microdilution Method

The MICs and minimum fungicidal concentrations (MFCs) of amphotericin B, fluconazole, ketoconazole, flucytosine, miconazole, and itraconazole for 12 isolates of filamentous opportunistic fungi (Scopulariopsis sp., Paecilomyces sp., Cladosporium spp., and Cladophialophora sp.) were determined by a broth microdilution method with hyphal and conidial inocula. With hyphal inocula MICs and MFCs were practically always substantially higher. Only 25% of the 60 MIC comparisons showed discrepancies of twofold or less, while the remaining comparisons showed much larger differences.

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