scholarly journals Correlation of Neo-Sensitabs Tablet Diffusion Assay Results on Three Different Agar Media with CLSI Broth Microdilution M27-A2 and Disk Diffusion M44-A Results for Testing Susceptibilities of Candida spp. and Cryptococcus neoformans to Amphotericin B, Caspofungin, Fluconazole, Itraconazole, and Voriconazole

2007 ◽  
Vol 45 (3) ◽  
pp. 858-864 ◽  
Author(s):  
A. Espinel-Ingroff ◽  
E. Canton ◽  
D. Gibbs ◽  
A. Wang
Keyword(s):  
Cryptococcus Neoformans ◽  
Amphotericin B ◽  
Disk Diffusion ◽  
Broth Microdilution ◽  
Candida Spp ◽  
Agar Media ◽  
Diffusion Assay

scholarly journals Wild-Type MIC Distributions and Epidemiological Cutoff Values for Amphotericin B, Flucytosine, and Itraconazole and Candida spp. as Determined by CLSI Broth Microdilution

2012 ◽  
Vol 50 (6) ◽  
pp. 2040-2046 ◽  
Author(s):  
M. A. Pfaller ◽  
A. Espinel-Ingroff ◽  
E. Canton ◽  
M. Castanheira ◽  
M. Cuenca-Estrella ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Broth Microdilution ◽  
Wild Type ◽  
Candida Spp ◽  
Cutoff Values

scholarly journals Categorizing susceptibility of clinical isolates of Candida auris to amphotericin B, caspofungin, and fluconazole utilizing the CLSI M44-A2 disk diffusion method

2021 ◽  
Author(s):  
Natalie S. Nunnally ◽  
Tajah Damm ◽  
Shawn R. Lockhart ◽  
Elizabeth L. Berkow
Keyword(s):  
Amphotericin B ◽  
Accurate Method ◽  
Clinical Isolates ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Broth Microdilution ◽  
Candida Auris ◽  
Disk Diffusion Method ◽  
Microdilution Method ◽  
Broth Microdilution Method

We evaluated the CLSI M44ed3E disk diffusion method in comparison with the CLSI M27ed4 broth microdilution method for caspofungin and fluconazole and the Etest method for amphotericin B to categorize susceptibility of 347 clinical isolates of Candida auris. Utilizing the zone diameter cutoffs established here we observed the overall categorial agreement between the two methods. For caspofungin, concordant results were observed for 98% of isolates with <1% very major and 1% major errors. For fluconazole, concordant results were observed for 91% of isolates with 1% very major and 8% major errors. For amphotericin B, concordant results were observed for 74% of isolates with <1% very major errors and 25% major errors. The disk diffusion approach provides an accurate method for determining the susceptibility of C. auris for caspofungin and fluconazole, and for identification of at least 75% of amphotericin B-susceptible isolates.

scholarly journals Comparison of MIC Test Strip and Sensititre YeastOne with the CLSI and EUCAST Broth Microdilution Reference Methods for In Vitro Antifungal Susceptibility Testing of Cryptococcus neoformans

2020 ◽  
Vol 64 (4) ◽  
Author(s):  
Fatima Zohra Delma ◽  
Abdullah M. S. Al-Hatmi ◽  
Jochem B. Buil ◽  
Hein van der Lee ◽  
Marlou Tehupeiory-Kooreman ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Amphotericin B ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Test Strip ◽  
Error Rates ◽  
Broth Microdilution ◽  
Content Type ◽  
In Vitro Antifungal Susceptibility

ABSTRACT We compared MIC test strip (MTS) and Sensititre YeastOne (SYO) methods with EUCAST and CLSI methods for amphotericin B, 5-fluocytosine, fluconazole, voriconazole, and isavuconazole against 106 Cryptococcus neoformans isolates. The overall essential agreement between the EUCAST and CLSI methods was >72% and >94% at ±1 and ±2 dilutions, respectively. The essential agreements between SYO and EUCAST/CLSI for amphotericin B, 5-flucytosine, fluconazole, and voriconazole were >89/>93% and between MTS and EUCAST/CLSI were >57/>75%. Very major error rates were low for amphotericin B and fluconazole (<3%) and a bit higher for the other drugs (<8%).

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