scholarly journals Efficacy of Pulsed-Field Gel Electrophoresis and Repetitive Element Sequence-Based PCR in Typing ofSalmonellaIsolates from Assam, India

2018 ◽  
Vol 56 (5) ◽  
Author(s):  
Purnima Gogoi ◽  
Probodh Borah ◽  
Iftikar Hussain ◽  
Leena Das ◽  
Girin Hazarika ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Repetitive Element ◽  
Pulsed Field Gel Electrophoresis ◽  
Fecal Samples ◽  
Content Type ◽  
Pulsed Field ◽  
Typing Methods ◽  
Element Sequence

ABSTRACTA total of 12Salmonellaisolates belonging to different serovars,viz.,Salmonella entericaserovar Enteritidis (n= 4),Salmonella entericaserovar Weltevreden (n= 4),Salmonella entericaserovar Newport (n= 1),Salmonella entericaserovar Litchifield (n= 1), and untypeable strains (n= 2) were isolated from 332 diarrheic fecal samples collected from animals, birds, and humans. Of the two molecular typing methods applied,viz., repetitive element sequence-based PCR (REP-PCR) and pulsed-field gel electrophoresis (PFGE), PFGE could clearly differentiate the strains belonging to different serovars as well as differentiate between strains of the same serovar with respect to their source of isolation, whereas REP-PCR could not differentiate between strains of the same serovar. Thus, it can be suggested that PFGE is more useful and appropriate for molecular typing ofSalmonellaisolates during epidemiological investigations than REP-PCR.

scholarly journals Evaluating Gulls as Potential Vehicles of Salmonella enterica Serotype Newport (JJPX01.0061) Contamination of Tomatoes Grown on the Eastern Shore of Virginia

2013 ◽  
Vol 80 (1) ◽  
pp. 235-238 ◽  
Author(s):  
Karen Gruszynski ◽  
Steven Pao ◽  
Chyer Kim ◽  
Denise M. Toney ◽  
Kim Wright ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Geographical Location ◽  
Pulsed Field Gel Electrophoresis ◽  
Dna Fingerprint ◽  
Eastern Shore ◽  
Fecal Samples ◽  
Content Type ◽  
Pulsed Field ◽  
Additional Information

ABSTRACTSalmonella entericaserovar Newport pattern JJPX01.0061 has been identified as causing several multistate outbreaks in the last 10 years, primarily due to contamination of tomatoes grown in Virginia. The goal of this study was to evaluate gulls as a potential vehicle ofS. Newport pattern 61 contamination for tomatoes grown on the Eastern Shore of Virginia. Gull fecal samples were collected at four sites in eastern Virginia for 3 months (May to July) in 2012, resulting in 360 samples, among whichSalmonellawas isolated from 62 samples. Twenty-two serotypes and 26 pulsed-field gel electrophoresis DNA fingerprint patterns, includingS. Newport pattern 61, were identified. All of the patterns that were isolated multiple times, with the exception ofS. Newport patterns JJPX01.0030 and JJPX01.0061, were clustered in time and geographical location. These results strongly suggest that both patterns ofS. Newport are endemic to sites on the Eastern Shore where gulls were sampled. This study provides additional information regarding the epidemiology ofS. Newport pattern 61 in Virginia and how tomatoes sold interstate may become contaminated in the field.

scholarly journals Discrimination between endemic and feedborne Salmonella Infantis infection in cattle by molecular typing

1999 ◽  
Vol 122 (3) ◽  
pp. 497-504 ◽  
Author(s):  
N. LINDQVIST ◽  
S. HEINIKAINEN ◽  
A.-M. TOIVONEN ◽  
S. PELKONEN
Keyword(s):  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Pulsed Field Gel Electrophoresis ◽  
S1 Nuclease ◽  
Cattle Feed ◽  
Pulsed Field ◽  
Secondary Infections ◽  
Cattle Farms

Salmonella enterica serovar Infantis is endemic in Finnish cattle. Feed contaminated with S. Infantis was distributed to cattle farms in May 1995. Following increased sampling, S. Infantis was detected on 242 farms in 1995. Molecular typing was used to differentiate the farms that were infected by the feed-related Infantis from those infected by other endemic strains. Twenty-three isolates from feed in 1995 and 413 from cattle (72 from 1992–4, 324 from 1995, 17 from 1996–7) were analysed. The feed-related Infantis was clonally related to the endemic infection by the ribotype, IS200-type and XbaI-profile. The feed isolates had a distinctive plasmid that appeared in pulsed-field gel electrophoresis as a 60 kb band when cleaved with XbaI or linearized by S1-nuclease. This plasmid appeared in cattle only since the outbreak and seemed stable on the follow-up farms. In addition to contact farms, the feedborne strain was found on 19% of the farms infected with S. Infantis in 1995 but not having bought suspected feedstuffs, possibly as secondary infections.

scholarly journals Pulsed-Field Gel Electrophoresis Supports the Presence of Host-Adapted Salmonella enterica subsp. enterica Serovar Typhimurium Strains in the British Garden Bird Population

2011 ◽  
Vol 77 (22) ◽  
pp. 8139-8144 ◽  
Author(s):  
Becki Lawson ◽  
Laura A. Hughes ◽  
Tansy Peters ◽  
Elizabeth de Pinna ◽  
Shinto K. John ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Phage Type ◽  
Pulsed Field Gel Electrophoresis ◽  
Phage Typing ◽  
Molecular Technique ◽  
Content Type ◽  
Pulsed Field ◽  
Phage Types ◽  
Serovar Typhimurium

ABSTRACTSalmonellosis is a frequently diagnosed infectious disease of passerine birds in garden habitats within Great Britain with potential implications for human and domestic animal health. Postmortem examinations were performed on 1,477 garden bird carcasses of circa 50 species from England and Wales, 1999 to 2007 inclusive. Salmonellosis was confirmed in 263 adult birds of 10 passerine species in this 11-year longitudinal study. A subset of 124 fully biotypedSalmonella entericasubsp.entericaserovar Typhimurium isolates was examined using pulsed-field gel electrophoresis to investigate the hypothesis that these strains are host adapted and to determine whether this molecular technique offers greater resolution in understanding the epidemiology ofSalmonellaTyphimurium infection than phage typing alone. For the two most common phage types, definitive type (DT) 40 and DT56v, which together accounted for 97% (120/124) of isolates, pulsed-field gel electrophoresis groupings closely correlated with phage type with remarkably few exceptions. A high degree of genetic similarity (>90%) was observed within and between the two most common pulsed-field gel electrophoresis groups. No clustering or variation was found in the pulsed-field gel electrophoresis groupings by bird species, year, or geographical region beyond that revealed by phage typing. These findings support the hypothesis that there are currently two host-adaptedSalmonellaphage types,S. Typhimurium DT40 and DT56v, circulating widely in British garden birds and that the reservoir of infection is maintained within wild bird populations. Large-scale multilocus sequence typing studies are required to further investigate the epidemiology of this infection.

scholarly journals A Whole-Genome Single Nucleotide Polymorphism-Based Approach To Trace and Identify Outbreaks Linked to a Common Salmonella enterica subsp. enterica Serovar Montevideo Pulsed-Field Gel Electrophoresis Type

2011 ◽  
Vol 77 (24) ◽  
pp. 8648-8655 ◽  
Author(s):  
Henk C. den Bakker ◽  
Andrea I. Moreno Switt ◽  
Craig A. Cummings ◽  
Karin Hoelzer ◽  
Lovorka Degoricija ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Pfge Pattern ◽  
Pulsed Field Gel Electrophoresis ◽  
Whole Genome ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Content Type ◽  
Pulsed Field

ABSTRACTIn this study, we report a whole-genome single nucleotide polymorphism (SNP)-based evolutionary approach to study the epidemiology of a multistate outbreak ofSalmonella entericasubsp.entericaserovar Montevideo. This outbreak included 272 cases that occurred in 44 states between July 2009 and April 2010. A case-control study linked the consumption of salami made with contaminated black and red pepper to the outbreak. We sequenced, on the SOLiD System, 47 isolates with XbaI PFGE pattern JIXX01.0011, a common pulsed-field gel electrophoresis (PFGE) pattern associated with isolates from the outbreak. These isolates represented 20 isolates collected from human sources during the period of the outbreak and 27 control isolates collected from human, food, animal, and environmental sources before the outbreak. Based on 253 high-confidence SNPs, we were able to reconstruct a tip-dated molecular clock phylogeny of the isolates and to assign four human isolates to the actual outbreak. We developed an SNP typing assay to rapidly discriminate between outbreak-related cases and non-outbreak-related cases and tested this assay on an extended panel of 112 isolates. These results suggest that only a very small percentage of the human isolates with the outbreak PFGE pattern and obtained during the outbreak period could be attributed to the actual pepper-related outbreak (20%), while the majority (80%) of the putative cases represented background cases. This study demonstrates that next-generation-based SNP typing provides the resolution and accuracy needed for outbreak investigations of food-borne pathogens that cannot be distinguished by currently used subtyping methods.

scholarly journals Evaluation of Genetic Markers and Molecular Typing Methods for Prediction of Sources of Campylobacter jejuni and C. coli Infections

2007 ◽  
Vol 73 (5) ◽  
pp. 1683-1685 ◽  
Author(s):  
Rauni Kärenlampi ◽  
Hilpi Rautelin ◽  
Marja-Liisa Hänninen
Keyword(s):  
Genetic Markers ◽  
Campylobacter Jejuni ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Marker Genes ◽  
Pulsed Field ◽  
Typing Methods

ABSTRACT Campylobacter jejuni and C. coli isolates from poultry, cattle, and humans were studied using pulsed-field gel electrophoresis (PFGE) and PCR of candidate livestock-associated marker genes. Human isolates showed 5.7 and 61% overlap with cattle and poultry isolates, respectively, by use of PFGE. No unambiguous association was found between marker genes (the Cj1321 and Cj1324 genes) and livestock-associated isolates.

Sign in / Sign up

Export Citation Format

Share Document