Enzyme-linked immunosorbent assay for detection of Salmonella typhi Vi antigen in urine from typhoid patients.

This study aims to determine the relationships between macrophage activities and interleukin-6 (IL-6 levels), and titer antibody to Salmonella typhi (S. typhi). A total of 32 mice were infected intraperitoneally with 105 cells/ml of S. typhi. The activities of macrophages wereobserved a day after the infection, while the observations of the levels of IL-6 and titer antibody employing enzyme-linked immunosorbent assay(ELISA) method were conducted after one and two weeks of infection. The results showed that the average activities of macrophages afterinfected by S. typhi was 77.84±11.23 sel, and the levels of IL-6 and antibody titers were higher (159.55±90.11 pg and 56.87±23.32 IUrespectively). The macrophage activities were significantly increased (P<0.01) with increasing the level of IL-6 to Y= -0.317x494.09 with the relatedness (r)= 0.89 and the antibody titers to Y= -0.020x2 + 0.00031x + 29.083 with the relatedness (r)= 0.95.Key words: macrophage activity, IL-6, antibody titers, Salmonella typhi2 + 0.0033x2 +

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Serodiagnosis of typhoid fever by enzyme-linked immunosorbent assay determination of anti-Salmonella typhi lipopolysaccharide antibodies.

Journal of Clinical Microbiology ◽

10.1128/jcm.20.4.718-721.1984 ◽

1984 ◽

Vol 20 (4) ◽

pp. 718-721 ◽

Cited By ~ 25

Author(s):

S Nardiello ◽

T Pizzella ◽

M Russo ◽

B Galanti

Keyword(s):

Typhoid Fever ◽

Immunosorbent Assay ◽

Salmonella Typhi ◽

Enzyme Linked Immunosorbent Assay

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Antibody Response to Somatic Antigen of Salmonella typhi in Areas Endemic and Non-Endemic for Typhoid Fever

Infection Control ◽

10.1017/s0195941700062779 ◽

1985 ◽

Vol 6 (3) ◽

pp. 110-114 ◽

Cited By ~ 3

Author(s):

Alexander Hirschl ◽

Gerold Stanek ◽

Manfred L. Rotter ◽

Pak Y. Chau ◽

Adolf H. Niemetz

Keyword(s):

Hong Kong ◽

Antibody Response ◽

Typhoid Fever ◽

Immunosorbent Assay ◽

Salmonella Typhi ◽

Serological Diagnosis ◽

Enzyme Linked Immunosorbent Assay ◽

Somatic Antigen ◽

Microagglutination Test ◽

Specific Igg

AbstractIn sera obtained between the 6th and the 30th day from 16 Austrian and 26 Hong Kong patients with culturally verified typhoid fever, agglutinating antibodies (microagglutination test) at significant titers were detected in 93% of the Austrian (median titer: 640) but in only 50% of the Hong Kong patients (median titer: 240). Similar results (93% and 54% positive sera respectively) were obtained for specific IgM as assessed by the enzyme-linked immunosorbent assay (ELISA) using lipopolysaccharide of S. typhi as antigen (median relative titer: 0.32 and 0.21 respectively). In contrast, specific IgG at significant concentrations were found in only 69% of the Austrian (median relative titer: 0.16) but 88% of the Hong Kong sera (median relative titer: 0.71). The (IgM-detecting) microagglutination test may be sufficiently diagnostic for typhoid fever in nonendemic areas such as Austria. In endemic regions like Hong Kong, however, tests indicative for early specific IgG are indispensable for serological diagnosis of the disease. The ELISA proved useful and is an example for such tests.

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