scholarly journals Comparison of the Raji cell line fluorescent antibody to membrane antigen test and the enzyme-linked immunosorbent assay for determination of immunity to varicella-zoster virus.

1982 ◽  
Vol 16 (5) ◽  
pp. 878-884 ◽  
Author(s):  
J P Iltis ◽  
G A Castellano ◽  
P Gerber ◽  
C Le ◽  
L K Vujcic ◽  
...  
Keyword(s):  
Varicella Zoster Virus ◽  
Cell Line ◽  
Immunosorbent Assay ◽  
Fluorescent Antibody ◽  
Raji Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigen Test ◽  
Varicella Zoster ◽  
Raji Cell Line

Infection and persistence of varicella-zoster virus in lymphoblastoid Raji cell line

1979 ◽  
Vol 167 (4) ◽  
pp. 275-283 ◽  
Author(s):  
S. Leventon-Kriss ◽  
T. Gotlieb-Stematsky ◽  
A. Vonsover ◽  
Z. Smetana
Keyword(s):  
Varicella Zoster Virus ◽  
Cell Line ◽  
Raji Cell ◽  
Varicella Zoster ◽  
Raji Cell Line

Brief Report Susceptibility of Filipino Nurses to the Varicella-Zoster Virus

1986 ◽  
Vol 7 (2) ◽  
pp. 71-72 ◽  
Author(s):  
Nabil T. Nassar ◽  
Helen C. Tourna
Keyword(s):  
Varicella Zoster Virus ◽  
Immunosorbent Assay ◽  
Medical Center ◽  
Young Female ◽  
Enzyme Linked Immunosorbent Assay ◽  
American University ◽  
Varicella Zoster ◽  
American University Of Beirut ◽  
Filipino Nurses ◽  
Risk Areas

AbstractFollowing an outbreak of varicella, 18% of a group of 174 young female Filipino nurses ranging in age from 20 to 25 years and working at the American University of Beirut Medical Center (AUMC) were found susceptible to the varicella-zoster virus; as compared to 3% of a matched group of 133 of their Lebanese colleagues. The level of antibody was determined by the Enzyme Linked Immunosorbent Assay (ELISA). Those susceptible were assigned duties in low-risk areas to varicella-zoster in the hospital.

scholarly journals Detection of Antibodies to Varicella-Zoster Virus in Recipients of the Varicella Vaccine by Using a Luciferase Immunoprecipitation System Assay

2014 ◽  
Vol 21 (9) ◽  
pp. 1288-1291 ◽  
Author(s):  
Jeffrey I. Cohen ◽  
Mir A. Ali ◽  
Ahmad Bayat ◽  
Sharon P. Steinberg ◽  
Hosun Park ◽  
...  
Keyword(s):  
Varicella Zoster Virus ◽  
High Throughput ◽  
Fluorescent Antibody ◽  
Varicella Vaccine ◽  
The United States ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Viral Capsid Antigen ◽  
Glycoprotein E ◽  
Varicella Zoster

ABSTRACTA high-throughput test to detect varicella-zoster virus (VZV) antibodies in varicella vaccine recipients is not currently available. One of the most sensitive tests for detecting VZV antibodies after vaccination is the fluorescent antibody to membrane antigen (FAMA) test. Unfortunately, this test is labor-intensive, somewhat subjective to read, and not commercially available. Therefore, we developed a highly quantitative and high-throughput luciferase immunoprecipitation system (LIPS) assay to detect antibody to VZV glycoprotein E (gE). Tests of children who received the varicella vaccine showed that the gE LIPS assay had 90% sensitivity and 70% specificity, a viral capsid antigen enzyme-linked immunosorbent assay (ELISA) had 67% and 87% specificity, and a glycoprotein ELISA (not commercially available in the United States) had 94% sensitivity and 74% specificity compared with the FAMA test. The rates of antibody detection by the gE LIPS and glycoprotein ELISA were not statistically different. Therefore, the gE LIPS assay may be useful for detecting VZV antibodies in varicella vaccine recipients. (This study has been registered at ClinicalTrials.gov under registration no. NCT00921999.)

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