Retrospective examination of lung tissue specimens for the presence of Legionella organisms: comparison of an indirect fluorescent-antibody system with direct fluorescent-antibody testing.

Rabbits were immunized with antigens extracted from mature spores or prespore stages of Myxosoma cerebralis, and the resulting antisera and their globulins were used in direct and indirect fluorescent antibody techniques. Both kinds of antisera reacted with homologous spores and with stages of the organism that precede spores. When tested for specificity against spores of 12 other myxosporidans the direct fluorescent antibody technique showed cross-reactivity with only one other and that was a species of Myxosoma. The indirect fluorescent antibody technique showed some reactions across generic lines. The antisera have application in studies of the parasite's life cycle and in diagnostics. Key words: spores, parasites, direct fluorescent antibody techniques, indirect fluorescent antibody techniques, diagnosis, myxosporidans, whirling disease

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Multiplex Viral Polymerase Chain Reaction Testing Using the FilmArray Device Compared With Direct Fluorescent Antibody Testing

Laboratory Medicine ◽

10.1309/lmomixq6n4japdx1 ◽

2014 ◽

Vol 45 (1) ◽

pp. 62-64 ◽

Cited By ~ 2

Author(s):

Jason Wang ◽

Debra B. Simons ◽

James L. Adams ◽

Robert C. Jerris ◽

Beverly Barton Rogers

Keyword(s):

Polymerase Chain Reaction ◽

Fluorescent Antibody ◽

Antibody Testing ◽

Chain Reaction ◽

Viral Polymerase ◽

Polymerase Chain Reaction Testing ◽

Direct Fluorescent Antibody ◽

Polymerase Chain

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A 34-year retrospective study of equine viral abortion in Poland

Polish Journal of Veterinary Sciences ◽

10.2478/pjvs-2014-0091 ◽

2014 ◽

Vol 17 (4) ◽

pp. 607-612 ◽

Cited By ~ 8

Author(s):

B.A. Bażanów ◽

A.B. Frącka ◽

N.A. Jackulak ◽

Z.M. Staroniewicz ◽

S.M. Ploch

Keyword(s):

Virus Isolation ◽

Indirect Fluorescent Antibody Test ◽

Fluorescent Antibody ◽

Antibody Test ◽

The Other ◽

Indirect Fluorescent Antibody ◽

Direct Fluorescent Antibody ◽

Tissue Homogenates ◽

Vero Cell Lines ◽

Isolation Test

Abstract The purpose of the present review was a comparison of the abortions caused by EAV and EHV-1 viruses over the 34 years. A total of 452 tissues samples from aborted fetuses (347) or foals (105) stillborn or newborn that died within 72 hours were investigated. The material for the examinations came from different farms located throughout Poland. The tissue homogenates were examined by using virus isolation test in RK-13 and Vero cell lines and the cytopathic agent was confirmed as EHV-1 by the direct fluorescent antibody test or as EAV by the indirect fluorescent antibody test. The study indicated that EAV was isolated (104 cases, 23%) almost as equally often as EHV-1 (116 cases, 25.6%). Both, equid herpesvirus-associated abortion and the abortion induced by EAV were characterized by cyclicity. The percentage of EAV and EHV-1 isolation alternately reduced and increased, but the increase of isolation of one virus was accompanied by the decrease of the other. The domination of one virus over the other occurred in cycles of a few years.

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