False-Positive Direct Fluorescent Antibody Testing for Legionella

JAMA ◽  
1985 ◽  
Vol 253 (1) ◽  
pp. 40
Author(s):  
Kenneth F. Wagner
Keyword(s):  
False Positive ◽  
Fluorescent Antibody ◽  
Antibody Testing ◽  
Direct Fluorescent Antibody ◽  
Positive Direct

COMPARISON OF SALMONELLA POLYVALENT H ANTISERA, DIRECT FLUORESCENT ANTIBODY, AND CULTURE PROCEDURES IN DETECTING SALMONELLAE FROM EXPERIMENTALLY CONTAMINATED GROUND BEEF UNDER FROZEN STORAGE

1974 ◽  
Vol 37 (7) ◽  
pp. 369-371
Author(s):  
W. C. Ladiges ◽  
J. F. Foster ◽  
W. M. Ganz
Keyword(s):  
False Positive ◽  
Fluorescent Antibody ◽  
False Negative ◽  
Frozen Storage ◽  
Ground Beef ◽  
Gram Negative ◽  
Selective Enrichment ◽  
Direct Fluorescent Antibody ◽  
False Position ◽  
Efficient Screening

A direct fluorescent antibody (FA) test and a polyvalent H agglutination (poly H) test with separate gram-negative (GN) and tetrathionate (TT) broth enrichments were compared with culture methodology in detecting salmonellae from experimentally contaminated ground beef. The FA test had the most false-position reactions (28%, but concurrent culture results reduced these to 7%) and the lowest number of false-negative reactions (4%). The poly H test was more efficient when TT broth was used for selective enrichment. On the basis of fewer false-negative reactions and the greater number of false-positive reactions that were culturally confirmed to be salmonellae, the FA test would appear to be a more efficient screening test in detecting salmonellae from artificially contaminated ground beef held at frozen temperatures.

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