Primary isolation and serial passage of hepatitis A virus strains in primate cell cultures.

1984 ◽  
Vol 20 (1) ◽  
pp. 28-33 ◽  
Author(s):  
L N Binn ◽  
S M Lemon ◽  
R H Marchwicki ◽  
R R Redfield ◽  
N L Gates ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Serial Passage ◽  
Primary Isolation ◽  
Primate Cell ◽  
Virus Strains

Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 238-239
Author(s):  
C.D. Humphrey ◽  
T.L. Cromeans ◽  
E.H. Cook ◽  
D.W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Liquid Phase ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immune Electron Microscopy ◽  
Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

Detection of Hepatitis a Virus and other Enteroviruses in Wastewater and Surface Water Samples by Gene Probe Assay

1991 ◽  
Vol 24 (2) ◽  
pp. 267-272 ◽  
Author(s):  
S. Dubrou ◽  
H. Kopecka ◽  
J. M. Lopez Pila ◽  
J. Maréchal ◽  
J. Prévot
Keyword(s):  
Surface Water ◽  
Cell Cultures ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Limit Of Detection ◽  
Blot Hybridization ◽  
Noncoding Region ◽  
Gene Probe ◽  
Dot Blot ◽  
Lower Efficiency

Enteroviruses were specifically detected by dot blot hybridization when using poliovirus type 1 (PV1) derived subgenomic radiolabeled cRNA probes (riboprobes) in environmental water specimens and in the cell cultures in which the viruses were amplificated. The riboprobe corresponding to the 5' noncoding sequence detected the majority of enteroviruses. Hepatitis A virus (HAV) was specifically detected by an HAV cRNA probe corresponding to the 5' noncoding region of its genome. By this test, the limit of detection of coxsackievirus B5 and echovirus 7 seeded in mineral water was 103 to 104 PFU/spot. In cell cultures, positive signals were observed in the lysates of cells infected by one PFU. Higher positive signals were obtained with a short PV1 probe (nt 221-670) corresponding to the 5' noncoding region, which is a well preserved sequence among the enteroviruses, than with PV1 genomic probe. Hybridization allowed a good detection of enteroviral RNAs in wastewater specimens, but with a lower efficiency in surface water. In this case, amplification of viruses in the cell cultures gave significant hybridization results.

scholarly journals Genetic Analysis of Hepatitis A Virus Strains That Induced Epidemics in Korea during 2007-2009

2012 ◽  
Vol 50 (4) ◽  
pp. 1252-1257 ◽  
Author(s):  
H. Lee ◽  
H. Jeong ◽  
H. Yun ◽  
K. Kim ◽  
J.-H. Kim ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Virus Strains

Hepatitis a virus: Growth characteristics of in vivo and in vitro propagated wild and attenuated virus strains

1984 ◽  
Vol 14 (4) ◽  
pp. 373-386 ◽  
Author(s):  
Daniel W. Bradley ◽  
Charles A. Schable ◽  
Karen A. McCaustland ◽  
E. H. Cook ◽  
Bert L. Murphy ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Growth Characteristics ◽  
Virus Growth ◽  
Attenuated Virus ◽  
Virus Strains

The complete genomes of subgenotype IA hepatitis A virus strains from four different islands in Indonesia form a phylogenetic cluster

2013 ◽  
Vol 159 (5) ◽  
pp. 935-945 ◽  
Author(s):  
Mulyanto ◽  
I Dewa Nyoman Wibawa ◽  
Joseph Benedictus Suparyatmo ◽  
Rifai Amirudin ◽  
Hiroshi Ohnishi ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Phylogenetic Cluster ◽  
Complete Genomes ◽  
Virus Strains
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