Recent vesicular stomatitis virus infection detected by immunoglobulin M antibody capture enzyme-linked immunosorbent assay.

ABSTRACT An immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (MC-ELISA) was developed for the detection of primary infection of vesicular stomatitis virus (VSV) in equine and swine sera. The test was based on the use of biotinylated sheep antibodies against equine or swine IgM molecules bound to a streptavidin-coated ELISA plate. The captured IgM antibodies were detected by application of antigens prepared from the New Jersey and the Indiana VSV serotypes (VSV-NJ and VSV-IN, respectively) and mouse polyclonal antibodies against VSV-NJ and VSV-IN. The MC-ELISA was compared to a competitive ELISA (C-ELISA) and the standard microtiter serum neutralization (MTSN) assay by testing serum samples from horses and pigs experimentally infected with VSV-NJ or VSV-IN. The MC-ELISA detected specific homologous IgM antibodies from equine and swine sera as early as 5 and 4 days postinfection (DPI), respectively, and as late as 35 DPI. The MTSN test also detected antibodies as early as 5 DPI and as late as 160 DPI. In a similar fashion, the C-ELISA detected antibodies from 6 to 7 DPI and as late as 160 DPI. These results demonstrated that the MC-ELISA is a useful test for serodiagnosis of primary VSV infection in horses and pigs.

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Comparison of the Serum Neutralization Test and a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Vesicular Stomatitis Virus New Jersey and Vesicular Stomatitis Virus Indiana

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063870201400309 ◽

2002 ◽

Vol 14 (3) ◽

pp. 240-242 ◽

Cited By ~ 8

Author(s):

Juan Francisco Alvarado ◽

Gaby Dolz ◽

Marco V. Herrero ◽

Brian McCluskey ◽

Mo Salman

Keyword(s):

New Jersey ◽

Confidence Interval ◽

Vesicular Stomatitis Virus ◽

Immunosorbent Assay ◽

Neutralization Test ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

Serum Neutralization ◽

Serum Neutralization Test ◽

Vesicular Stomatitis

A competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of antibodies against vesicular stomatitis virus New Jersey (VSV-NJ) and vesicular stomatitis virus Indiana (VSV-IN) was compared with the serum neutralization test (SNT) using 1,106 serum samples obtained from dairy cattle on sentinel study farms in the Poás region of Costa Rica. Kappa coefficients between the C-ELISA and the SNT were 0.8871 (95% confidence interval [CI]: 0.8587–0.9155) and 0.6912 (95% CI: 0.6246–0.7577) for the VSV-NJ and VSV-IN tests, respectively. These results indicate good to excellent agreement between the 2 tests under these conditions.

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Interlaboratory evaluation of indirect enzyme-linked immunosorbent assay, antibody capture enzyme-linked immunosorbent assay, and immunoblotting for detection of immunoglobulin M antibodies to Toxoplasma gondii.

Journal of Clinical Microbiology ◽

10.1128/jcm.25.1.100-105.1987 ◽

1987 ◽

Vol 25 (1) ◽

pp. 100-105 ◽

Cited By ~ 23

Author(s):

P Herbrink ◽

A M van Loon ◽

J P Rotmans ◽

F van Knapen ◽

W C van Dijk

Keyword(s):

Toxoplasma Gondii ◽

Immunosorbent Assay ◽

Immunoglobulin M ◽

Enzyme Linked Immunosorbent Assay ◽

Antibody Capture

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Characterization of pseudorabies virus antibody responses in young swine after infection and vaccination by using an immunoglobulin M antibody capture enzyme-linked immunosorbent assay.

Journal of Clinical Microbiology ◽

10.1128/jcm.30.2.346-350.1992 ◽

1992 ◽

Vol 30 (2) ◽

pp. 346-350 ◽

Cited By ~ 5

Author(s):

M B McCaw ◽

T W Molitor ◽

H S Joo

Keyword(s):

Immunosorbent Assay ◽

Pseudorabies Virus ◽

Immunoglobulin M ◽

Antibody Responses ◽

Enzyme Linked Immunosorbent Assay ◽

Virus Antibody ◽

Antibody Capture

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Development of a Human-Murine Chimeric Immunoglobulin M for Use in the Serological Detection of Human Alphavirus Antibodies

Clinical and Vaccine Immunology ◽

10.1128/cvi.05269-11 ◽

2011 ◽

Vol 18 (12) ◽

pp. 2181-2182 ◽

Cited By ~ 5

Author(s):

Brett A. Thibodeaux ◽

Nathan M. Liss ◽

Amanda N. Panella ◽

John T. Roehrig

Keyword(s):

Monoclonal Antibody ◽

Immunosorbent Assay ◽

Immunoglobulin M ◽

Murine Monoclonal Antibody ◽

Enzyme Linked Immunosorbent Assay ◽

Serological Detection ◽

Antibody Capture

ABSTRACTDiagnosis of human alphaviral infections relies on serological techniques, such as the immunoglobulin M antibody capture–enzyme-linked immunosorbent assay (MAC-ELISA). We have humanized the alphavirus broadly cross-reactive murine monoclonal antibody 1A4B-6 to create a reagent capable of replacing human positive sera in the MAC-ELISA for diagnosis of human alphaviral infections.

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