scholarly journals Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Detection, Typing, and Subtyping of Vesicular Stomatitis Virus

1991 ◽  
Vol 3 (4) ◽  
pp. 287-292 ◽  
Author(s):  
Albino Alonso ◽  
Mauricio A. Martins ◽  
Maria da Penha D. Gomes ◽  
Rossana Allende ◽  
Magnus S. Söndahl
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vesicular Stomatitis

scholarly journals Comparison of the Serum Neutralization Test and a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Vesicular Stomatitis Virus New Jersey and Vesicular Stomatitis Virus Indiana

2002 ◽  
Vol 14 (3) ◽  
pp. 240-242 ◽  
Author(s):  
Juan Francisco Alvarado ◽  
Gaby Dolz ◽  
Marco V. Herrero ◽  
Brian McCluskey ◽  
Mo Salman
Keyword(s):  
New Jersey ◽  
Confidence Interval ◽  
Vesicular Stomatitis Virus ◽  
Immunosorbent Assay ◽  
Neutralization Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Serum Neutralization ◽  
Serum Neutralization Test ◽  
Vesicular Stomatitis

A competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of antibodies against vesicular stomatitis virus New Jersey (VSV-NJ) and vesicular stomatitis virus Indiana (VSV-IN) was compared with the serum neutralization test (SNT) using 1,106 serum samples obtained from dairy cattle on sentinel study farms in the Poás region of Costa Rica. Kappa coefficients between the C-ELISA and the SNT were 0.8871 (95% confidence interval [CI]: 0.8587–0.9155) and 0.6912 (95% CI: 0.6246–0.7577) for the VSV-NJ and VSV-IN tests, respectively. These results indicate good to excellent agreement between the 2 tests under these conditions.

scholarly journals Development of an Immunoglobulin M (IgM) Capture Enzyme-Linked Immunosorbent Assay for Detection of Equine and Swine IgM Antibodies to Vesicular Stomatitis Virus

2001 ◽  
Vol 8 (3) ◽  
pp. 475-481 ◽  
Author(s):  
En-min Zhou ◽  
Jose Riva ◽  
Alfonso Clavijo
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Immunosorbent Assay ◽  
Polyclonal Antibodies ◽  
Competitive Elisa ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Igm Antibodies ◽  
Vesicular Stomatitis ◽  
Elisa Plate

ABSTRACT An immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (MC-ELISA) was developed for the detection of primary infection of vesicular stomatitis virus (VSV) in equine and swine sera. The test was based on the use of biotinylated sheep antibodies against equine or swine IgM molecules bound to a streptavidin-coated ELISA plate. The captured IgM antibodies were detected by application of antigens prepared from the New Jersey and the Indiana VSV serotypes (VSV-NJ and VSV-IN, respectively) and mouse polyclonal antibodies against VSV-NJ and VSV-IN. The MC-ELISA was compared to a competitive ELISA (C-ELISA) and the standard microtiter serum neutralization (MTSN) assay by testing serum samples from horses and pigs experimentally infected with VSV-NJ or VSV-IN. The MC-ELISA detected specific homologous IgM antibodies from equine and swine sera as early as 5 and 4 days postinfection (DPI), respectively, and as late as 35 DPI. The MTSN test also detected antibodies as early as 5 DPI and as late as 160 DPI. In a similar fashion, the C-ELISA detected antibodies from 6 to 7 DPI and as late as 160 DPI. These results demonstrated that the MC-ELISA is a useful test for serodiagnosis of primary VSV infection in horses and pigs.

An enzyme-linked immunosorbent assay for the detection of vesicular stomatitis virus antibodies

1992 ◽  
Vol 14 (3-4) ◽  
pp. 293-301 ◽  
Author(s):  
R. Allende ◽  
L. Sepúlveda ◽  
A. Mendes da Silva ◽  
M. Martins ◽  
M.S. Söndahl ◽  
...  
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vesicular Stomatitis

scholarly journals Enzyme-Linked Immunosorbent Assay Using Glycoprotein and Monoclonal Antibody for Detecting Antibodies to Vesicular Stomatitis Virus Serotype New Jersey

2009 ◽  
Vol 16 (5) ◽  
pp. 667-671 ◽  
Author(s):  
Hyang-Sim Lee ◽  
Eun-Jeong Heo ◽  
Hye-Young Jeoung ◽  
Hyo-Rim Ko ◽  
Chang-Hee Kweon ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
New Jersey ◽  
Disease Virus ◽  
Vesicular Stomatitis Virus ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Swine Vesicular Disease Virus ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Vesicular Stomatitis

ABSTRACT In this study, an enzyme-linked immunosorbent assay (ELISA) using glycoprotein and a monoclonal antibody (MAb) was developed for the detection of antibodies to vesicular stomatitis virus (VSV) serotype New Jersey (NJ). The glycoprotein to be used as a diagnostic antigen was extracted from partially purified VSV-NJ, and a neutralizing MAb specific to VSV-NJ was incorporated to compete with antibodies in a blocking ELISA using glycoprotein (GP ELISA). The cutoff of the GP ELISA was set at 40% inhibition, which corresponded to a virus neutralization test (VNT) titer of 32. With this threshold, the GP ELISA exhibited 99.6% specificity for naïve sera (n = 3,005) from cattle (n = 1,040), pigs (n = 1,120), and horses (n = 845) from domestic farms. The GP ELISA did not cross-react with sera positive for foot-and-mouth disease virus, swine vesicular disease virus, or VSV serotype Indiana. The GP ELISA was more compatible with the VNT than was the nucleocapsid-based ELISA for VSV-NJ-positive sera (n = 19). Taken together, this GP ELISA could be a useful tool as an alternative to the VNT for detecting antibodies specific to VSV-NJ.

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