Protein G-based enzyme-linked immunosorbent assay for anti-MPB70 antibodies in bovine tuberculosis.

1990 ◽  
Vol 28 (5) ◽  
pp. 913-921 ◽  
Author(s):  
M Harboe ◽  
H G Wiker ◽  
J R Duncan ◽  
M M Garcia ◽  
T W Dukes ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Bovine Tuberculosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein G

A novel fusion protein-based indirect enzyme-linked immunosorbent assay for the detection of bovine tuberculosis

Tuberculosis ◽  
2007 ◽  
Vol 87 (3) ◽  
pp. 212-217 ◽  
Author(s):  
Siguo Liu ◽  
Sheping Guo ◽  
Chunlai Wang ◽  
Meili Shao ◽  
Xiuhua Zhang ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Immunosorbent Assay ◽  
Bovine Tuberculosis ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Detection of False-Positive Sera in Contagious Agalactia with a Multiantigen ELISA and their Elimination with a Protein G Conjugate

1998 ◽  
Vol 10 (4) ◽  
pp. 326-330 ◽  
Author(s):  
Maurice Lambert ◽  
Michel Calamel ◽  
Philippe Dufour ◽  
Evelyne Cabasse ◽  
Christian Vitu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein G ◽  
Mycoplasma Agalactiae ◽  
Cross Reactions ◽  
Serologic Diagnosis ◽  
Contagious Agalactia ◽  
The Past

In serology, lack of specificity can generally be attributed to cross-reactions between different pathogens with antigens bearing similar epitopes. During seroepidemiologic surveys of contagious agalactia of sheep caused by Mycoplasma agalactiae infection, numerous sera were analyzed by enzyme-linked immunosorbent assay (ELISA). A few sera reacted with various antigens coated on plates, including the well with no antigen. This reactivity was not due to cross-reactions as initially suspected, and these multipositive sera were designated false-positive sera. Elimination of this false positivity was not possible by using covalent ELISA plates or different rabbit anti-sheep IgG conjugates. Only conjugates using monoclonal antibodies or protein G were efficient in elimination of false positivities without reducing the true specific positive titers. No false-positive sera have been observed since the implementation of protein G conjugates in the serologic diagnosis of contagious agalactia by ELISA for the past 2 years.

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