Enzyme Linked Immunosorbent Assay Using Alkaline Phosphatase Conjugated with Streptococcal Protein G

1988 ◽  
Vol 9 (2) ◽  
pp. 207-225 ◽  
Author(s):  
Bo Nilson ◽  
Lars Björck ◽  
Bo Åkerström
Keyword(s):  
Alkaline Phosphatase ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein G ◽  
Streptococcal Protein G

A solid-phase enzyme-linked immunosorbent assay for the quantitation of human plasma alpha 1-acid glycoprotein.

1979 ◽  
Vol 25 (4) ◽  
pp. 546-549 ◽  
Author(s):  
H P Wang ◽  
C Y Chu
Keyword(s):  
Alkaline Phosphatase ◽  
Human Plasma ◽  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Solid Phase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Normal Range ◽  
Oral Contraceptive Pills ◽  
Acid Glycoprotein ◽  
Contraceptive Pills

Abstract We describe a solid-phase enzyme-linked immunosorbent assay for alpha 1-acid glycoprotein in human plasma. Plasma samples are incubated with alkaline phosphatase-linked, purified alpha 1-acid glycoprotein in alpha 1-acid glycoprotein-specific antibody-coated polystyrene tubes. The alkaline phosphatase that becomes attached to the tube via an immunological reaction between the alpha 1-acid glycoprotein and the specific antibody is measured spectrophotometrically. This assay is accurate reproducible, simple, and economical. As little as 4 microgram of alpha 1-acid glycoprotein per liter can be detected. The normal range for alpha 1-acid glycoprotein in the plasma of healthy adults, as measured by this method, is 0.48-1.27 g/L; the range is significantly different, 0.29-0.73 g/L, for women who are taking oral contraceptive pills.

Protein G-based enzyme-linked immunosorbent assay for anti-MPB70 antibodies in bovine tuberculosis.

1990 ◽  
Vol 28 (5) ◽  
pp. 913-921 ◽  
Author(s):  
M Harboe ◽  
H G Wiker ◽  
J R Duncan ◽  
M M Garcia ◽  
T W Dukes ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Bovine Tuberculosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein G

scholarly journals Detection of False-Positive Sera in Contagious Agalactia with a Multiantigen ELISA and their Elimination with a Protein G Conjugate

1998 ◽  
Vol 10 (4) ◽  
pp. 326-330 ◽  
Author(s):  
Maurice Lambert ◽  
Michel Calamel ◽  
Philippe Dufour ◽  
Evelyne Cabasse ◽  
Christian Vitu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein G ◽  
Mycoplasma Agalactiae ◽  
Cross Reactions ◽  
Serologic Diagnosis ◽  
Contagious Agalactia ◽  
The Past

In serology, lack of specificity can generally be attributed to cross-reactions between different pathogens with antigens bearing similar epitopes. During seroepidemiologic surveys of contagious agalactia of sheep caused by Mycoplasma agalactiae infection, numerous sera were analyzed by enzyme-linked immunosorbent assay (ELISA). A few sera reacted with various antigens coated on plates, including the well with no antigen. This reactivity was not due to cross-reactions as initially suspected, and these multipositive sera were designated false-positive sera. Elimination of this false positivity was not possible by using covalent ELISA plates or different rabbit anti-sheep IgG conjugates. Only conjugates using monoclonal antibodies or protein G were efficient in elimination of false positivities without reducing the true specific positive titers. No false-positive sera have been observed since the implementation of protein G conjugates in the serologic diagnosis of contagious agalactia by ELISA for the past 2 years.

Sign in / Sign up

Export Citation Format

Share Document