scholarly journals Assessment of Protein G in Serodiagnosis of Zoo Animals and Development of an Enzyme-Linked Immunosorbent Assay for Asian Elephant

2008 ◽  
Vol 61 (1) ◽  
pp. 75-78
Author(s):  
Kentaro SHIMOSAWA ◽  
Naoaki MISAWA
Keyword(s):  
Immunosorbent Assay ◽  
Asian Elephant ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein G ◽  
Zoo Animals

Protein G-based enzyme-linked immunosorbent assay for anti-MPB70 antibodies in bovine tuberculosis.

1990 ◽  
Vol 28 (5) ◽  
pp. 913-921 ◽  
Author(s):  
M Harboe ◽  
H G Wiker ◽  
J R Duncan ◽  
M M Garcia ◽  
T W Dukes ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Bovine Tuberculosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein G

scholarly journals Detection of False-Positive Sera in Contagious Agalactia with a Multiantigen ELISA and their Elimination with a Protein G Conjugate

1998 ◽  
Vol 10 (4) ◽  
pp. 326-330 ◽  
Author(s):  
Maurice Lambert ◽  
Michel Calamel ◽  
Philippe Dufour ◽  
Evelyne Cabasse ◽  
Christian Vitu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein G ◽  
Mycoplasma Agalactiae ◽  
Cross Reactions ◽  
Serologic Diagnosis ◽  
Contagious Agalactia ◽  
The Past

In serology, lack of specificity can generally be attributed to cross-reactions between different pathogens with antigens bearing similar epitopes. During seroepidemiologic surveys of contagious agalactia of sheep caused by Mycoplasma agalactiae infection, numerous sera were analyzed by enzyme-linked immunosorbent assay (ELISA). A few sera reacted with various antigens coated on plates, including the well with no antigen. This reactivity was not due to cross-reactions as initially suspected, and these multipositive sera were designated false-positive sera. Elimination of this false positivity was not possible by using covalent ELISA plates or different rabbit anti-sheep IgG conjugates. Only conjugates using monoclonal antibodies or protein G were efficient in elimination of false positivities without reducing the true specific positive titers. No false-positive sera have been observed since the implementation of protein G conjugates in the serologic diagnosis of contagious agalactia by ELISA for the past 2 years.

Comparison of polyclonal, monoclonal and protein G peroxidase conjugates in an enzyme-linked immunosorbent assay for the diagnosis of Brucella ovis in sheep

1998 ◽  
Vol 143 (14) ◽  
pp. 390-394 ◽  
Author(s):  
C. M. Marin ◽  
B. Alonso-Urmeneta ◽  
I. Moriyon ◽  
S. Perez-Gomez ◽  
J. M. Blasco
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein G ◽  
Brucella Ovis

Development of an immunological method for studying the incorporation of ripening enzymes in cheese curd

1996 ◽  
Vol 63 (1) ◽  
pp. 141-149 ◽  
Author(s):  
Edith Laloy ◽  
Jean-Christophe Vuillemard ◽  
Mouhsine El Abboudi ◽  
Ismael Fliss ◽  
Eric De Grace ◽  
...  
Keyword(s):  
Detection Limit ◽  
Immunosorbent Assay ◽  
Total Protein ◽  
Lactobacillus Casei ◽  
Polyclonal Antibodies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein G ◽  
Immunological Method ◽  
Liposome Suspension ◽  
Cheese Curd

SummaryPolyclonal antibodies raised against partly purified aminopeptidase were specific to cell-free extracts ofLactobacillus caseisubsp.pseudoplantarumUL 137, and did not cross react with any other proteins in Cheddar cheese, at least during the first week of maturation. A sandwich enzyme-linked immunosorbent assay (ELISA) was developed in order to quantify cell-free extracts in the cheese curd, and was also used to determine the efficiency of encapsulation of cell-free extracts in liposomes. This method was very sensitive and exhibited a detection limit of ∼10 μg total protein/g cheese and ∼1 μg total protein/ml liposome suspension. The efficiency of encapsulation of cell-free extracts into liposomes was ∼55–60%. The retention of liposome-encapsulated cell-free extracts was ∼14 times that of non-encapsulated extracts.

Sign in / Sign up

Export Citation Format

Share Document