scholarly journals Evaluation of a commercially available enzyme-linked immunosorbent assay for Giardia lamblia antigen in stool.

1991 ◽  
Vol 29 (6) ◽  
pp. 1137-1142 ◽  
Author(s):  
D G Addiss ◽  
H M Mathews ◽  
J M Stewart ◽  
S P Wahlquist ◽  
R M Williams ◽  
...  
PEDIATRICS ◽  
1994 ◽  
Vol 93 (1) ◽  
pp. 28-31
Author(s):  
Juan N. Walterspiel ◽  
Ardythe L. Morrow ◽  
Larry K. Pickering ◽  
Guillermo M. Ruiz-Palacios ◽  
M. Lourdes Guerrero

Objective. To determine whether anti-Giardia lamblia secretory IgA (sIgA) antibodies in human milk protect infants from acquisition of or symptoms associated with Giardia infection. Methods. One hundred ninety-seven Mexican mother/infant pairs were followed weekly from birth for diarrheal disease and feeding status. Infant stool specimens were collected weekly and were cultured for bacterial pathogens and tested for Giardia and rotavirus by enzyme-linked immunosorbent assay. Maternal milk samples were collected weekly for 1 month postpartum and monthly thereafter. To determine the protective effect of anti-Giardia sIgA in milk against infection and against diarrhea due to Giardia, milk samples from mothers of infected infants and appropriately matched controls were assayed for anti-Giardia sIgA by enzyme-linked immunosorbent assay. Results. Asymptomatic, infected infants ingested significantly (P = .046) higher amounts of milk anti-Giardia sIgA compared with symptomatic, infected infants. However, milk anti-Giardia sIgA concentrations did not differ between Giardia-infected and noninfected infants. Conclusion. The amount of anti-Giardia sIgA in human milk was associated with prevention of symptoms of diarrhea due to Giardia, but not with acquisition of the organism.


PubVet ◽  
2021 ◽  
Vol 15 (6) ◽  
pp. 1-8
Author(s):  
Felipe de Paula Sá ◽  
Lucas Cavalcante de Moura ◽  
Pablo Luiz Marins Mota ◽  
Daniela Mello Vianna Ferrer

A giardíase é classificada como uma doença de caráter social, sua epidemiologia está relacionada diretamente com o perfil socioeconômico da população. O protozoário nomeado como Giardia lamblia é o responsável pelos quadros de diarreia, desidratação, distensão, dores abdominais e perda de peso. Isso se deve pela fixação da forma trofozoíta no enterócito, além da sua dispersão por toda região do duodeno. Através do contato entre seres humanos e animais o ciclo da giárdia pode promover uma infecção zoonótica, por conta das inúmeras fontes de infecção, destacando-se a contaminação oro-fecal ou a ingestão de água contaminada. Diferentes métodos de diagnóstico são utilizados para averiguar se o animal está positivo para giárdia, sendo o método imunoenzimático ELISA (Enzyme Linked Immunosorbent Assay) considerado mais fidedigno para este caso. Entre os inúmeros protocolos de tratamento descritos pela literatura, fica evidente a primazia pelo uso dos fármacos: metronidazol, tinidazol e furazolidona. A profilaxia consiste na manutenção de bons hábitos higiênicos pessoais, ressaltando principalmente, a lavagem correta das mãos como o mais eficiente. O presente trabalho tem por objetivo realizar uma revisão de literatura sobre a giardíase, comentando sua etiologia, aspectos epidemiológicos, patogenia, sinais clínicos, formas de diagnóstico e tratamento. Dessa forma, visando enriquecer as informações sobre a doença para profissionais de saúde e buscando atualizar o debate sobre a mesma, ressaltando sua importância.


2013 ◽  
Vol 9 (2) ◽  
pp. 177-182 ◽  
Author(s):  
MSH Suman ◽  
MM Alam ◽  
SB Pun ◽  
A Khair ◽  
S Ahmed ◽  
...  

Giardia lamblia is highly infectious protozoan parasite capable of causing gastrointestinal illness in both humans and animals. The objective of this study was to determine the prevalence of Giardia lamblia infection in children < 5 years old and calves. Enzyme Linked Immunosorbent Assay (ELISA) has been used for the detection of Giardia lamblia. A total of 266 children and 15 calves diarrheic fecal samples were tested for Giardia lamblia during January 2011 to May 2012. The prevalence of Giardia lamblia infection among children was 3.8% while 13.3% in calves. Giardia lamblia was highest in children between 24 and 60 months of age (8.7%). Giardia lamblia infection was higher in male (4.7%) than in female (2.0%). Male calves (14.3%) have slightly higher prevalence than female calves (12.5%). The highest prevalence (33.3%) of Giardia lamblia infection in calves was between the ages 6 and 9 months. This is the first study to determine the prevalence of Giardia lamblia infection in calves using ELISA method in Bangladesh. A larger scale study is needed for accurate estimates of prevalence of Giardia lamblia to undertake an appropriate control strategy in future.DOI: http://dx.doi.org/10.3329/bjvm.v9i2.13474


Author(s):  
Ana Cristina Berne ◽  
Juliana Nunes Vieira ◽  
Luciana Farias da Costa De Avila ◽  
Marcos Marreiro Villela ◽  
Maria Elisabeth Aires Berne ◽  
...  

1984 ◽  
Vol 149 (1) ◽  
pp. 90-97 ◽  
Author(s):  
B. L. P. Ungar ◽  
R. H. Yolken ◽  
T. E. Nash ◽  
T. C. Quinn

1981 ◽  
Vol 80 (6) ◽  
pp. 1476-1480 ◽  
Author(s):  
Phillip D. Smith ◽  
Frances D. Gillin ◽  
William R. Brown ◽  
Theodore E. Nash

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