scholarly journals Diagnosis by AMPLICOR PCR of Chlamydia trachomatis infection in urine samples from women and men attending sexually transmitted disease clinics.

1996 ◽  
Vol 34 (6) ◽  
pp. 1401-1406 ◽  
Author(s):  
T C Quinn ◽  
L Welsh ◽  
A Lentz ◽  
K Crotchfelt ◽  
J Zenilman ◽  
...  
2020 ◽  
Author(s):  
Xinlong Chen ◽  
Yanling Gan ◽  
Qiu Chen ◽  
Zhuo Ren ◽  
Furong Li ◽  
...  

Abstract Background Genital chlamydia trachomatis infection is one of the most prevalent sexually transmitted disease globally. The diagnosis and reporting of genital chlamydia trachomatis infection mainly rely on effective laboratory testing available. The prevalence of genital chlamydia trachomatis infection in China may be largely underestimated, based on the reported data. Methods A survey was conducted among hospitals provided clinical services for STD in Shandong Province in 2018. Basic information on the hospitals, type of laboratory tests provided and cases reported of GCTI were collected and analysed in SPSS 22. Results Among 456 hospitals surveyed, antigen testing, PCR testing, and either antigen or PCR testing were available among 200 (43.9%), 88 (19.3%) and 268 (58.8%) hospitals, respectively. PCR testing was more available among tertiary and secondary hospitals than primary hospitals (X 2 =28.560, P=0.000). Significant differences were observed in the availability of antigen testing, PCR testing, and either antigen or PCR testing among different type of hospitals (X 2 =15.708, P=0.003; X 2 =22.494, P=0.000; X 2 =21.729, P=0.000). In 2018, a total of 1532 cases of GCTI were reported in 99 hospitals. More cases were reported by tertiary and secondary hospitals than primary hospitals (X 2 =24.082, P=0.000). The proportion of different kind of hospitals that reported case of GCTI cases was consistent with that of hospitals provided laboratory testing for GCTI. Conclusion The availability of laboratory testing for GCTI in Shandong province was poor, which may lead to underestimation of prevalence of GCTI. More effort needs to improve the availability of GCTI laboratory testing in order to effectively control of GCTI in Shandong province.


1998 ◽  
Vol 36 (6) ◽  
pp. 1489-1493 ◽  
Author(s):  
Mirja Puolakkainen ◽  
Eija Hiltunen-Back ◽  
Timo Reunala ◽  
Satu Suhonen ◽  
Pekka Lähteenmäki ◽  
...  

The diagnostic performance of a PCR test (Roche Cobas Amplicor CT/NG Test) and that of a ligase chain reaction (LCR) test (Abbott LCxChlamydia trachomatis assay) were compared by using endocervical and urethral swab specimen culture as a reference test. First-void urine (FVU) and endocervical and urethral swab specimens were collected from 1,015 unselected patients attending a sexually transmitted disease clinic and a clinic for adolescents in Helsinki, Finland. Chlamydia trachomatis was cultured from samples from the endocervix or urethra. PCR was performed with fresh and frozen urine and the culture transport medium. LCR was performed with fresh and frozen urine and LCx swab transport medium. Diagnostic consistency and diagnostic accuracy were statistically tested. The test results were identical for 984 patients (97%). Discrepant results were observed for 31 patients. Overall, LCR and PCR showed excellent kappa coefficients of consistency for both swab and FVU specimens (0.93 and 0.95, respectively). Sixty-one patients (6%) were culture positive. Testing of FVU by LCR or PCR increased the overall positivity rates to 7.0 and 7.7%, respectively. While PCR of FVU detected the greatest number of C. trachomatis infections (sensitivity, 96.1%), for some PCR-positive FVU specimens the results could not be confirmed (specificity, 99.6%). PCR and LCR were more sensitive than culture (sensitivities, 92 and 93% versus 79% for culture) in the diagnosis of genital C. trachomatis infection. In conclusion, both tests can be recommended for use in the clinical laboratory and for the screening of asymptomatic C. trachomatis infections.


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